Seed banking of Polish endangered plants – the FlorNatur Project

Puchalski, Jerzy; Niemczyk, Maciej; Walerowski, Piotr; Podyma, Wiesław; Kapler, Adam

doi:10.2478/biorc-2014-0005

Cited by 8 publications

(3 citation statements)

References 18 publications

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“…Also, ex situ conservation measures need to be undertaken to preserve this new identified pool of species variability. As ex situ conservation measure we suggest collection of mature seeds and preservation in seed bank, following the Polish example (Puchalski et al 2014). Another efficient conservation measure could be cultivation in botanical gardens, parks, private gardens, etc., the species being very decorative, fragrant and easy to grow (Cullen et al 2011).…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity within a newly identified population of Adenophora liliifolia (L.) A.DC. in Romania: implications for conservation

Manole¹,

Banciu²,

Indreica

2015

Ann. For. Res.

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Abstract. Adenophora liliifolia is a relict plant species, endangered at European level. Its occurrence in Romania is limited to a very few sites. The amount of genetic diversity of plant species is a valuable indicator of population, being the baseline in developing proper strategies for their conservation. Inter-simple sequence repeats (ISSRs) markers were used to analyze polymorphism in A. liliifolia genome and to evaluate the genetic diversity and accordingly, the state of a recently identified population. Five ISSR primers, specially designed for plants, and containing different simple sequence repeat motifs were tested. A total of 52 ISSR fragments were generated of which 41 were polymorphic (78.84%) and 32 (61.53%) specific to Adenophora genus. The value of Shannon's index of genotypic diversity was 0.812. Jaccard similarity coefficient was calculated for pair wise comparisons among all individuals and ranged from 0.17 to 0.83. The genetic variability between individuals was 78.84% which suggests a relative high genetic differentiation. Although the level of genetic variability is moderate to high, the population is declining and exposed to demographic stochasticity. A possible cause is species germination requirements hampered by modification in vegetation structure and abundance. The population survival and reinforcement is conditioned by urgent measures for forest management in order to reduce herbaceous and shrubby vegetation and to limit mowing and grazing. Ex situ conservation measures are also proposed.

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Section: Discussionmentioning

confidence: 99%

Genetic diversity within a newly identified population of Adenophora liliifolia (L.) A.DC. in Romania: implications for conservation

Manole¹,

Banciu²,

Indreica

2015

Ann. For. Res.

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“…Long-term seed storage methods are based on standardized protocols developed by botanical gardens worldwide in cooperation with the Royal Botanic Gardens, Kew. However, nearly all protocol steps have been re-optimized for many species with the knowledge of dormancy breaking and freezing tolerance of the seeds (Niemczyk & Puchalski, 2015;Puchalski et al, 2014). A germination test was performed for each set of seeds collected in the field and delivered to the seed bank before storage in LN and aer 15-20 years of cryopreservation for controlled seed samples.…”

Section: The Polish Academy Of Sciences Botanical Garden -Center For ...mentioning

confidence: 99%

Polish Cryobanks: Research and Conservation of Plant Genetic Resources

et al. 2022

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To date, the use of liquid nitrogen (LN) in plant gene banks is relatively limited. Globally, in 2021, approximately 10,000 accessions were cryopreserved, and their explants were derived from in vitro plants. In Europe, out of 500 banks, only 20 use cryogenic temperatures to store plant germplasms. The implementation of seven conservation projects in Poland starting in the 1990s meant that the gene banking system in this country began to gradually develop. Plant gene bank is mainly based on the collection of seeds and makes little use of plant tissues, including those from in vitro culture. From the point of view of systematics, plant material belonging to spermatophytes dominates in Polish gene banks, while spore plants are rarely represented. This review represents the first summary of gene cryobank activities and resources in Poland. It contains a brief overview of the ex situ plant protection programs aimed at the use of LN and presents the characteristics of four existing gene cryobanks in this country. Special attention is devoted to the presentation of studies on the cryopreservation of seeds, as well as of plant tissues, such as embryonic axes and plumules of trees, and fern gametophytes, that make a permanent contribution to gene bank resources.

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“…It aims not only at preserving the natural populations in situ but also at developing appropriate techniques for ex situ preservation (creation of a sterile tissue culture, appropriate storage of seeds in seed banks, experimental germination tests and cultivation in order to identify the critical factors for seedling growth). The other countries of Central Europe suggested a similar approach to protect this species, although without the official funded conservation programs (e.g., Kucharczyk, 2007;Puchalski et al, 2014;Manole et al, 2015).…”

Section: Conservation Of a Liliifoliamentioning

confidence: 99%

Adenophora liliifolia: Condition of its Populations in Central Europe

Prausová

Marečková

Kapler

et al. 2016

Acta Biologica Cracoviensia S. Botanica

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This study deals with populations of the European-South-Siberian geoelement Adenophora liliifolia (L.) A. DC. in the Czech Republic, Slovakia, Hungary, Romania, and Poland, where this species has its European periphery distribution. We studied the population size, genetic variability, site conditions, and vegetation units in which A. liliifolia grows. Recent and historical localities of A. liliifolia were ranked into six vegetation units of both forest and non-forest character. A phytosociological survey showed differences in the species composition among localities. Only a weak pattern of population structure was observed (only 22% of total genetic variation present at the interpopulation level, AMOVA analysis), with moderate values for gene diversity (H j = 0.141) and polymorphism (P = 27.6%). Neighborjoining and Bayesian clusterings suggest a similar genetic background for most of the populations from Slovakia, the Czech Republic, and Poland, contrary to the populations from Hungary, Romania, as well as two populations from Central and South Slovakia. This might be explained by a relatively recent fragmentation of the A. liliifolia populations in Central Europe. Nevertheless, it seems that several populations in Romania, South Hungary, and Slovakia were isolated for a longer period of time and their genetic differentiation is more evident.

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Seed banking of Polish endangered plants – the FlorNatur Project

Cited by 8 publications

References 18 publications

Genetic diversity within a newly identified population of Adenophora liliifolia (L.) A.DC. in Romania: implications for conservation

Genetic diversity within a newly identified population of Adenophora liliifolia (L.) A.DC. in Romania: implications for conservation

Polish Cryobanks: Research and Conservation of Plant Genetic Resources

Adenophora liliifolia: Condition of its Populations in Central Europe

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