Sedimenticola selenatireducens, gen. nov., sp. nov., an anaerobic selenate-respiring bacterium isolated from estuarine sediment

Narasingarao, Priya; Häggblom, Max M.

doi:10.1016/j.syapm.2005.12.011

Cited by 68 publications

(49 citation statements)

References 22 publications

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“…With the exception for the clone IAFJAsip25 affiliated with Sedimenticola selenatireducens, which is known to reduce nitrate and nitrite under denitrifying conditions (Narasingarao and Haggblom, 2006), none of clones were affiliated with bacteria known to denitrify or to reduce nitrate into nitrite. However, one sequence was related to Bacteriovorax sp., which suggests that bacteria devourers were present in the biofilm that probably fed on the denitrifying methylotrophic bacteria.…”

Section: Discussionmentioning

confidence: 97%

Dissimilatory reduction of nitrate in seawater by a Methylophaga strain containing two highly divergent narG sequences

et al. 2010

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Methylophaga spp. are methylotrophs commonly associated with marine environments and have been defined as strict aerobic methylotrophs. They have been shown previously to represent 50-70% of the bacterial population in the biofilm of the methanol-fed denitrification reactor treating a large seawater aquarium at the Montreal Biodome. It was therefore surprising to find such a high concentration of Methylophaga spp. in anoxic conditions. In this study, we showed by cultivationindependent and -dependent approaches that one Methylophaga strain present in the anoxic biofilm is involved in the denitrification process. DNA stable-isotope probing (SIP) experiments in which the biofilm was cultured under denitrifying conditions with 13 C-methanol have revealed the enrichment of one particular taxon. By screening a 16S ribosomal RNA gene library derived from a 13 C-DNA fraction of the SIP gradients, 62% of the library was composed of one sequence affiliated with the genus Methylophaga. One strain, named JAM1, representing this Methylophaga species was isolated. It grows aerobically but also under denitrifying conditions by reducing nitrate into nitrite. The nitrate-reducing activity was correlated with the presence and the expression of two highly divergent narG genes (narG1 and narG2). narG1 showed a high percentage of identity with the corresponding part of narG found in Thiobacillus denitrificans, which suggests a recent acquisition of narG in strain JAM1 by horizontal gene transfer. This study provides the first direct evidence of the adaptation of a Methylophaga species to an oxygen-limited environment.

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Section: Discussionmentioning

confidence: 97%

Dissimilatory reduction of nitrate in seawater by a Methylophaga strain containing two highly divergent narG sequences

et al. 2010

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“…Electron microscopy was performed at the electron microscopy facility at the Nelson Biology Laboratory, Rutgers University, as described previously (35). Scanning electron microscopy was performed by directly mounting the sample on filter discs.…”

Section: Methodsmentioning

confidence: 99%

Identification of Anaerobic Selenate-Respiring Bacteria from Aquatic Sediments

Narasingarao

Häggblom

2007

Appl Environ Microbiol

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102

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The diversity population of microorganisms with the capability to use selenate as a terminal electron acceptor, reducing it to selenite and elemental selenium by the process known as dissimilatory selenate reduction, is largely unknown. The overall objective of this study was to gain an in-depth understanding of anaerobic biotransformation of selenium in the environment, particularly anaerobic respiration, and to characterize the microorganisms catalyzing this process. Here, we demonstrate the isolation and characterization of four novel anaerobic dissimilatory selenate-respiring bacteria enriched from a variety of sources, including sediments from three different water bodies in Chennai, India, and a tidal estuary in New Jersey. Strains S5 and S7 from India, strain KM from the Meadowlands, NJ, and strain pn1, categorized as a laboratory contaminant, were all phylogenetically distinct, belonging to various phyla in the bacterial domain. The 16S rRNA gene sequence shows that strain S5 constitutes a new genus belonging to Chrysiogenetes, while strain S7 belongs to the Deferribacteres, with greater than 98% 16S rRNA gene similarity to Geovibrio ferrireducens. Strain KM is related to Malonomonas rubra, Pelobacter acidigallici, and Desulfuromusa spp., with 96 to 97% 16S rRNA gene similarity. Strain pn1 is 99% similar to Pseudomonas stutzeri. Strains S5, S7, and KM are obligately anaerobic selenate-respiring microorganisms, while strain pn1 is facultatively anaerobic. Besides respiring selenate, all these strains also respire nitrate.

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“…These bacteria were isolated from the Arthur Kill and the Kesterson Reservoir (28). Narasingarao and Häggblom enriched and isolated dissimilatory SeO 4 2Ϫ -respiring bacteria from geographically and characteristically different sediment samples from Chennai (India) and New Jersey by using SeO 4 2Ϫ as the sole terminal electron acceptor (47,48). Four bacterial strains were isolated that could grow via dissimilatory SeO 4 2Ϫ respiration and belonged to metabolically and taxonomically diverse taxa of the Gammaproteobacteria, Deltaproteobacteria, Deferribacteres, and Chrysiogenetes.…”

Section: Selenate-respiring Bacteriamentioning

confidence: 99%

Ecology and Biotechnology of Selenium-Respiring Bacteria

Nancharaiah

2015

Microbiol Mol Biol Rev

348

232

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SUMMARY In nature, selenium is actively cycled between oxic and anoxic habitats, and this cycle plays an important role in carbon and nitrogen mineralization through bacterial anaerobic respiration. Selenium-respiring bacteria (SeRB) are found in geographically diverse, pristine or contaminated environments and play a pivotal role in the selenium cycle. Unlike its structural analogues oxygen and sulfur, the chalcogen selenium and its microbial cycling have received much less attention by the scientific community. This review focuses on microorganisms that use selenate and selenite as terminal electron acceptors, in parallel to the well-studied sulfate-reducing bacteria. It overviews the significant advancements made in recent years on the role of SeRB in the biological selenium cycle and their ecological role, phylogenetic characterization, and metabolism, as well as selenium biomineralization mechanisms and environmental biotechnological applications.

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Sedimenticola selenatireducens, gen. nov., sp. nov., an anaerobic selenate-respiring bacterium isolated from estuarine sediment

Cited by 68 publications

References 22 publications

Dissimilatory reduction of nitrate in seawater by a Methylophaga strain containing two highly divergent narG sequences

Dissimilatory reduction of nitrate in seawater by a Methylophaga strain containing two highly divergent narG sequences

Identification of Anaerobic Selenate-Respiring Bacteria from Aquatic Sediments

Ecology and Biotechnology of Selenium-Respiring Bacteria

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