Sedimentation Field-Flow Fractionation of Cellular Species

Métreau, Jean Marc; Gallet, Sacha; Cardot, Philippe; Maire, Valérie Le; Dumas, Frédéric; Hernvann, Alain; Loric, Sylvain

doi:10.1006/abio.1997.2271

Cited by 31 publications

(10 citation statements)

References 19 publications

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“…[1][2][3] For these purposes, conventional flowassisted separation techniques and their microfabricated counterparts have been developed, such as field flow fractionation (FFF) and split-flow thin fractionation (SF). [4][5][6][7][8][9][10][11][12] Among the various FFF methods, sedimentation FFF (SdFFF), gravitational FFF (GrFFF), and their combination method with dielectrophoresis (DEP-FFF) were mainly used for applications of micron-sized particles, providing biocompatible and sterilized protocols. [5][6][7][8][9][10] However, particle separation in GrFFF and DEP-FFF is not conducted in a continuous manner, which gives some disadvantages of both restricted amounts of samples within separation chamber volumes and relatively long separation times.…”

Section: Introductionmentioning

confidence: 99%

“…[4][5][6][7][8][9][10][11][12] Among the various FFF methods, sedimentation FFF (SdFFF), gravitational FFF (GrFFF), and their combination method with dielectrophoresis (DEP-FFF) were mainly used for applications of micron-sized particles, providing biocompatible and sterilized protocols. [5][6][7][8][9][10] However, particle separation in GrFFF and DEP-FFF is not conducted in a continuous manner, which gives some disadvantages of both restricted amounts of samples within separation chamber volumes and relatively long separation times. [7][8][9][10] SF, one of the continuous fractionation methods, was applied to separation of human blood cells using centrifugal and magnetic fields.…”

Section: Introductionmentioning

confidence: 99%

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Continuous hydrophoretic separation and sizing of microparticles using slanted obstacles in a microchannel

2007

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We report a microfluidic separation and sizing method of microparticles with hydrophoresis--the movement of suspended particles under the influence of a microstructure-induced pressure field. By exploiting slanted obstacles in a microchannel, we can generate a lateral pressure gradient so that microparticles can be deflected and arranged along the lateral flows induced by the gradient. Using such movements of particles, we completely separated polystyrene microbeads with 9 and 12 microm diameters. Also, we discriminated polystyrene microbeads with diameter differences of approximately 7.3%. Additionally, we measured the diameter of 10.4 microm beads with high coefficient of variation and compared the result with a conventional laser diffraction method. The slanted obstacle as a microfluidic control element in a microchannel is analogous to the electric, magnetic, optical, or acoustic counterparts in that their function is to generate a field gradient. Since our method is based on intrinsic pressure fields, we could eliminate the need for external potential fields to induce the movement of particles. Therefore, our hydrophoretic method will offer a new opportunity for power-free and biocompatible particle control within integrated microfluidic devices.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Continuous hydrophoretic separation and sizing of microparticles using slanted obstacles in a microchannel

2007

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“…Parts a and b of Figure 2 show that, whatever the type of particle and ∆T, R increases steadily with increasing carrier flow rate. This kind of behavior is typical of that observed for retention of micrometer-sized particles in sedimentation FFF, either gravitational or centrifugal, 34,35,[52][53][54][55][56][57][58][59] and implicitly in symmetrical flow FFF channels. 60 This behavior has been interpreted as a result of the lift mechanism of retention in FFF.…”

Section: Resultsmentioning

confidence: 71%

Influence of Operating Parameters On the Retention of Chromatographic Particles By Thermal Field-Flow Fractionation

2004

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We have investigated the retention behavior of chromatographic particles in thermal field-flow fractionation (FFF). Retention time is found to increase with increasing temperature drop across the channel thickness, as expected for species exhibiting a thermophoretic mobility. Experiments have been performed with a vertically oriented channel rather than by using the classical horizontal configuration as this leads to much more reproducible retention data. In acetonitrile, silica-based particles are more retained than octadecyl-bonded silica particles, which confirms our previous finding, by means of a different method, that the thermophoretic mobility of the latter is smaller than that of the former. Whatever the type of particles and the nature of the carrier liquid, the relative retention time is observed to decrease with increasing carrier flow rate. This indicates that a hydrodynamic lift force acts on particles to move them away from the accumulation wall, as is usually observed in all FFF experiments with micrometer-sized particles. However, upward and downward flow directions in the vertical channel lead to similar retention data, indicating that inertial lift forces have a minor influence on retention. In addition, the relative retention time steadily decreases with increasing sample concentration, suggesting that the hydrodynamic lift force increases significantly with sample concentration. Accordingly, we speculate that a new transport phenomenon, called shear-induced hydrodynamic diffusion, not previously accounted for in the modeling of retention in FFF, is controlling the migration of the particles in the FFF channel. Implications of the influence of this phenomenon in other FFF experiments are discussed.

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“…7,8 Sedimentation field-flow fractionation is a powerful technique for the fractionation and characterization of particles and colloids in environmental and biological samples. 5,9,10 For environmental samples, colloidal particles in river water, 11 colloidal aggregates, 12 soil particles, 13 and airborne samples [5][6][7][8]14,15 were investigated.…”

Section: Introductionmentioning

confidence: 99%

Sedimentation field-flow fractionation-inductively coupled plasma optical emission spectrometry: size-based elemental speciation of air particulates

Kumtabtim

Shiowatana

Siripinyanond

2005

J. Anal. At. Spectrom.

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Sedimentation Field-Flow Fractionation of Cellular Species

Cited by 31 publications

References 19 publications

Continuous hydrophoretic separation and sizing of microparticles using slanted obstacles in a microchannel

Continuous hydrophoretic separation and sizing of microparticles using slanted obstacles in a microchannel

Influence of Operating Parameters On the Retention of Chromatographic Particles By Thermal Field-Flow Fractionation

Sedimentation field-flow fractionation-inductively coupled plasma optical emission spectrometry: size-based elemental speciation of air particulates

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