Sedimentation‐equilibrium studies of the polysaccharide components of <i>Pseudomonas aeruginosa</i>

Horton, Derek; Riley, David; Hansen, P.M.T.

doi:10.1002/bip.1980.360191009

Cited by 4 publications

(2 citation statements)

References 37 publications

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“…One can calculate that the mole percent of smooth-type molecules in P. aeruginosa LPS varies from 0.2 to 13.7 mol% of the total LPS (calculated from data [2, 30] using molecular weights of 14,000 and 3,000, respectively, for smooth and rough LPS oligosaccharides [13]). This agrees with an independent calculation of 5 to 11 mol% by Wilkinson in a recent review (S. G. Wilkinson, Rev.…”

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confidence: 99%

Pseudomonas aeruginosa isolates from patients with cystic fibrosis: a class of serum-sensitive, nontypable strains deficient in lipopolysaccharide O side chains

Hancock¹,

Mutharia²,

Chan³

et al. 1983

Infect Immun

426

192

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Twenty-six Pseudomonas aeruginosa strains from patients with cystic fibrosis were typed by the Fisher immunotyping scheme. Only 6 strains were agglutinated by a single typing serum, whereas 15 strains were agglutinated with more than one serum and 5 were not agglutinated by any serum. Neither the polyagglutinable nor the nonagglutinable strains were typable by hemagglutination inhibition or immunodiffusion, suggesting that these polyagglutinable strains did not express multiple serotype antigens, but were instead being agglutinated by antibody to nonserotype determinants. Four typable isolates were resistant to pooled normal human serum, whereas the 12 polyagglutinable and nonagglutinable isolates studied were very sensitive to normal human serum. The outer membranes of 16 strains were isolated and characterized. The data suggested, in general, strong conservation of outer membrane protein patterns. Lipopolysaccharides (LPS) were purified by a new technique which allowed isolation of both rough and smooth LPS in high yields. Three of four typable, serum-resistant strains examined had amounts of smooth, O-antigen-containing LPS equivalent to our laboratory wild type, P. aeruginosa PAO1 strain H103. In contrast, 10 of 12 polyagglutinable or nonagglutinable, serum-sensitive strains had very little or no smooth, O-antigen-containing LPS, and the other two contained less smooth LPS than our wild-type strain H103. In agreement with this data, five independent, rough, LPS O-antigen-deficient mutants of strain H103 were nontypable and serum sensitive. We suggest that the LPS defects described here represent a significant new property of many P. aeruginosa strains associated with cystic fibrosis.

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confidence: 99%

Pseudomonas aeruginosa isolates from patients with cystic fibrosis: a class of serum-sensitive, nontypable strains deficient in lipopolysaccharide O side chains

Hancock¹,

Mutharia²,

Chan³

et al. 1983

Infect Immun

426

192

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“…Interference patterns were recorded after equilibrium was reached (24 to 28 h). Weight average molecular weights were calculated with a value of 0.58 for the partial specific volume of the polysaccharide, an average value for polysaccharides computed from data given in Horton et al (14).…”

Section: Molecular Weight Determinadon a Spinco Model Ementioning

confidence: 99%

Two distinct classes of polyuronide from the cell walls of a dimorphic fungus, Mucor rouxii

Dow¹,

Darnall²,

Villa³

1983

J Bacteriol

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Polyuronides were extracted from purified yeast and mycelial walls of Mucor rouxii by sequential treatments with lithium chloride and potassium hydroxide and were fractionated by ion-exchange chromatography on DEAE-Sephadex. Two polymers (I and II) of different acidity were found in both wall types. Polymer I contained D-glucuronic acid, L-fucose, D-mannose, and much smaller amounts of D-galactose. Yeast and mycelial polymer I had similar uronic acid contents but differed in their neutral sugar compositions and molecular weights. Polymer II from both cell types contained largely D-glucuronic acid and had similar molecular weights. On partial acid hydrolysis, both polymers I and II gave rise to insoluble glucuronans which appeared to be homopolymeric. One-third of the total uronosyl residues of polymer I, and almost all of the uronosyl residues of polymer II, were present in homopolymeric segments. However, homopolymers derived from polymers I and II may not be identical.

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31P nuclear magnetic resonance spectroscopy of lipopolysaccharides from pseudomonas aeruginosa

Horton

Riley

1981

Biochimica et Biophysica Acta (BBA) - Biomembranes

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Sedimentation‐equilibrium studies of the polysaccharide components of Pseudomonas aeruginosa

Cited by 4 publications

References 37 publications

Pseudomonas aeruginosa isolates from patients with cystic fibrosis: a class of serum-sensitive, nontypable strains deficient in lipopolysaccharide O side chains

Pseudomonas aeruginosa isolates from patients with cystic fibrosis: a class of serum-sensitive, nontypable strains deficient in lipopolysaccharide O side chains

Two distinct classes of polyuronide from the cell walls of a dimorphic fungus, Mucor rouxii

31P nuclear magnetic resonance spectroscopy of lipopolysaccharides from pseudomonas aeruginosa

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