Sedimentation characteristics of newly synthesized Epstein-Barr viral DNA in superinfected cells

Siegel, Pamela J.; Clough, Wendy; Strominger, Jack L.

doi:10.1128/jvi.38.3.880-885.1981

Cited by 8 publications

(5 citation statements)

References 21 publications

(41 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…(The experiments were done before recombinant EBV DNA clones were available.) Siegel and co-workers looked at the size of replicating viral DNA in superinfected Raji cells (14); there appeared to be several size classes, one of which could possibly be defective DNA. Why does standard HR-1 DNA not replicate upon superinfection of X50-7 cells?…”

Section: R P3r3mentioning

confidence: 99%

P3HR-1 Epstein-Barr virus with heterogeneous DNA is an independent replicon maintained by cell-to-cell spread

Miller¹,

Heston²,

Countryman³

1985

J Virol

View full text Add to dashboard Cite

We present results of biological experiments which indicate that the subpopulation of Epstein-Barr virus strain P3HR-1 with heterogeneous (het) DNA consists of self-contained replicons which multiply alongside, but independently of, Epstein-Barr virus strain HR-1 containing standard DNA. When a population of HR-1 virions containing het DNA was introduced into X50-7 cells, the input heterogeneous DNA increased in abundance, as did the DNA of the endogenous virus of X50-7 cells. The input standard HR-1 viral DNA, however, was not amplified. When parental HR-1 cells or a cellular subclone containing het DNA were grown for several weeks in the presence of human serum with neutralizing antibody, the het DNA was lost from the culture; standard HR-1 DNA, however, was not affected by antiserum. Furthermore, virions containing het DNA could be serially propagated through cellular subclones of HR-1 cells which lack het DNA. After each serial passage, cells which acquired het DNA released virions with the ability to induce early antigens in Raji cells. These experiments define a novel in vitro life cycle of an Epstein-Barr virus variant which is maintained, not vertically by partitioning to daughter cells in cell division, but horizontally by cell-to-cell spread.

show abstract

Section: R P3r3mentioning

confidence: 99%

P3HR-1 Epstein-Barr virus with heterogeneous DNA is an independent replicon maintained by cell-to-cell spread

Miller¹,

Heston²,

Countryman³

1985

J Virol

View full text Add to dashboard Cite

show abstract

“…Epstein-Barr virus is spontaneously reactivated leading to lytic antigen expression. In cases in which lytic antigen expression culminates in the production of virions, the presence of linear genomes can be demonstrated (22). In vitro studies have shown that acyclovir is effective only in reducing the number of linear replicative Epstein-Barr virus genomes (19,23).…”

Section: Discussionmentioning

confidence: 99%

The Diagnosis of Epstein‐Barr Virus‐Associated Polymorphic B Cell Lymphoma in Immunocompromised Patients: Review of Methods

Beauparlant

Alfieri

Joncas

1990

Canadian Journal of Infectious Diseases and Medical Microbiolog

View full text Add to dashboard Cite

Polymorphic B cell lymphoma and diffuse B cell lymphoproliferation associated with Epstein-Barr virus infection is increasingly reported in immunodeficient patients. Accurate diagnosis of these pathologies is essential because the appropriate treatment regimens for the patients in question differ from those for patients with other lymphoproliferative diseases. Two complementary techniques are currently used in the diagnosis and characterization of Epstein-Barr virus-associated B cell lymphomas and diffuse B cell lymphoproliferation. Immunofluorescence allows specific detection of Epstein-Barr nuclear antigens in lymphomatous tissue. Molecular hybridization with the Bam H1-W and/or Bam H1-NJ probes confirms the presence of the Epstein-Barr virus genome in tumour cells. The Bam H1-NJ probe is also useful in determining the clonality of the tumour and the replication mode, episomal or linear, of the viral genome. The polymerase chain reaction method allows detection of the Epstein-Barr virus genome within 24 h in these tumours and is more sensitive.

show abstract

“…When latency is disrupted by cryptic signals that occasionally activate viral genes (21) during the early S phase (22), a multitude of virus-induced proteins is expressed, including a DNA polymerase (11,12,17,39) which amplifies the number of copies of circular EBV DNA (50). The circular genomes synthesized by the viral enzyme (50,53) are presumably precursors of the linear DNA in virions.…”

mentioning

confidence: 99%

Maintenance of growth transformation with Epstein-Barr virus is mediated by secretion of autocrine growth factors in two serum-free B-cell lines

Shaw

Baglia

Leung

1988

J Virol

View full text Add to dashboard Cite

show abstract

Sedimentation characteristics of newly synthesized Epstein-Barr viral DNA in superinfected cells

Cited by 8 publications

References 21 publications

P3HR-1 Epstein-Barr virus with heterogeneous DNA is an independent replicon maintained by cell-to-cell spread

P3HR-1 Epstein-Barr virus with heterogeneous DNA is an independent replicon maintained by cell-to-cell spread

The Diagnosis of Epstein‐Barr Virus‐Associated Polymorphic B Cell Lymphoma in Immunocompromised Patients: Review of Methods

Maintenance of growth transformation with Epstein-Barr virus is mediated by secretion of autocrine growth factors in two serum-free B-cell lines

Contact Info

Product

Resources

About