Sediment toxicity tests using benthic marine microalgae Cylindrotheca closterium (Ehremberg) Lewin and Reimann (Bacillariophyceae)

Moreno-Garrido, Ignácio; Hampel, Miriam; Lubián, Luís M.; Blasco, Julián

doi:10.1016/s0147-6513(02)00077-5

Cited by 38 publications

(19 citation statements)

References 25 publications

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“…However, no toxicity data have been reported regarding diuron in sediments. Marine benthic diatom (Cylindrotheca closterium) showed high sensitivity, and the EC50 value was reported to be 26 mg/kg Cu spiked in natural marine sand [6], which is lower than the EC50 values from the present study for coarsegrained sediments (142 mg Cu/kg) and fine-grained sediment (1,189 mg Cu/kg). Previous studies also reported some lethal toxicity data such as median lethal concentration values of Cu-spiked sediments to Tubifex tubifex ranging from 88 to 106 mg/kg [30], median lethal concentration of 1,310 mg Cu/kg for adult and 790 mg Cu/kg for juvenile amphipod Melita plumulosa [31], and median lethal concentration values of 426 mg Cu/kg for Chironomus riparius larvae after 10 d of exposure to Cu-spiked sediments [32].…”

Section: Species Sensitivitycontrasting

confidence: 85%

Development and application of whole‐sediment toxicity test using immobilized freshwater microalgae Pseudokirchneriella subcapitata

Zhang

Ying

Chen

et al. 2011

Enviro Toxic and Chemistry

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Abstract-A method for a whole-sediment toxicity test using alginate immobilized microalgae Pseudokirchneriella subcapitata was developed using spiked sediments and applied to contaminated field sediment samples. For method development, a growth inhibition test (72 h) with algal beads was conducted for the sediments spiked with Cu or diuron. The method was validated by determining doseresponse relationships for Cu and diuron in both fine-grained and coarse-grained sediments. The results of a spiked sediment toxicity test suggested that sediment particle size distribution (clay content) had a significant effect on the growth of P. subcapitata. The developed method using immobilized microalgae P. subcapitata beads was applied successfully in the toxicity test and toxicity identification evaluation (TIE) for the four field sediment samples. After a series of extractions with 0.01 M CaCl 2 solution, acetone, and dichloromethane, the extracted sediment, which was shown to be nontoxic to algae, was used as the control and diluent for the same sediment in the whole-sediment toxicity test. The results showed that all investigated field sediment samples were found to be toxic to the immobilized algae P. subcapitata, with their median effective concentration (EC50) values ranging from 41.4 to 79.0% after 72 h exposure. In the whole sediment TIE, growth of P. subcapitata was improved to varying degrees after adding zeolite, resin, or activated charcoal, suggesting different contributions to toxicity from ammonia, metals, and organic contaminants in the tested sediments.

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Section: Species Sensitivitycontrasting

confidence: 85%

Development and application of whole‐sediment toxicity test using immobilized freshwater microalgae Pseudokirchneriella subcapitata

Zhang

Ying

Chen

et al. 2011

Enviro Toxic and Chemistry

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“…Despite previous studies (Moreno‐Garrido et al ) reporting no effect of Cd on C. closterium at concentrations in sediment below 20 mg kg −1 , the presence of Cd does appear to restrict diatom growth. The mass of diatoms retrieved from the contaminated and uncontaminated treatments without sediment was similar at the 3‐h time point; however, at 24 and 96 h, diatom mass in the contaminated water treatment remained stable, while in the uncontaminated water treatment diatom mass increased over time.…”

Section: Discussioncontrasting

confidence: 66%

Cadmium uptake from sediment by Cylindrotheca closterium and the effect of diatom presence on partitioning of cadmium between sediment and water: A laboratory study

Becker

Copplestone

2019

Limnology & Oceanography

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Although it is well established that microalgae take up metals and other contaminants from water and it has been suggested that algae may play a significant role in mobilizing sediment bound contaminants, there has been little research on the uptake of sediment‐associated contaminants by microalgae. This may be important for microphytobenthos, which is closely associated with sediments. We report on laboratory experiments investigating the uptake of cadmium (Cd) from sediment and water by Cylindrotheca closterium over 96 h. The role of microalgae in the partitioning of Cd between sediment and water was also investigated. While concentrations do not typically represent those in the natural environment, we showed C. closterium takes up Cd from sediment, and concentration in microalgae is affected by sediment organic matter content. Cylindrotheca closterium influenced Cd movement between sediment and water: transfer from water to sediment was slowed, while transfer from sediment to overlying water (all treatments) and interstitial water (unprocessed sediment treatments) was increased. This is the first article to describe Cd uptake by diatoms from intertidal sediment in relation to sediment properties and mobilization of Cd from sediment in the presence of diatoms. Microalgae may serve as a pathway for sediment‐associated metals to enter into aquatic food webs, and their presence appears to increase metal concentrations in water potentially making any mobilized metals available for uptake by other species. Given this and their importance as the basis of the food chain, there may be implications for environmental and human health and potential impacts for the biological stability of the sediment.

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“…They were established considering previous studies (e.g. Moreira-Santos et al 2002;Moreno-Garrido et al 2003) and the requirements of the OECD guideline for toxicity testing with microalgae (OECD 2011). Following previous optimization of procedures, an aliquot of an exponentially growing culture, concentrated by gravity rather than by centrifugation, was added to each alginate solution (1.3 and 1.5 %) to obtain an alginate-cell suspension with ca.…”

Section: Cell Immobilizationmentioning

confidence: 99%

“…Although international guidelines such as OECD (2011), ISO8692 (1989) and USEPA (2002) mentioned the use of diatoms for ecotoxicological proposes, their use in ecotoxicological risk assessment as well as the development of methodologies for its use have been neglected and the focus is given to macrophytes and planktonic freshwater microalgae. Notwithstanding, much less interest has been paid to the species that compose the microphytobenthos and the development of methodologies for its use in ecotoxicological testing (Araújo et al 2010;Moreno-Garrido et al 2003;SETAC 1993). In fact, planktonic microalgae show high sensitivity to toxicants, often being more sensitive than other planktonic organisms (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…The immobilization of the diatoms in encapsulation matrices offers many advantages over the use of free cells, especially for in situ toxicity assessment as stated by Araújo et al (2010). The encapsulation of microalgae has been successfully used in toxicity tests developed for application in freshwater (Moreira-Santos et al 2002;Moreira-Santos et al 2004b), estuarine and marine ecosystems (Moreno-Garrido et al 2003, 2007.…”

Section: Introductionmentioning

confidence: 99%

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Optimization of growth conditions for laboratory and field assessments using immobilized benthic diatoms

Vidal

Marques

Abrantes

et al. 2014

Environ Sci Pollut Res

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The availability of rapid and effective methodologies for assessing lotic systems with microphytobenthos is still quite scarce. Hence, the primary goal of this study was to optimize the growth conditions of the sensitive and ubiquous benthic diatom Navicula libonensis for laboratorial and field assessments. The effect of different conditions of temperature, photoperiod, initial cell density, test duration and cell encapsulation into calcium alginate beads was evaluated in a first set of experiments. There was a slight increase in the growth of free and immobilized cells at 23 °C, at lower initial cell densities and at the shortest experimental period (6 days). Through all the conditions, the growth profiles of free versus immobilized were fairly variable. A second experimental trial involved the validation of selected conditions, applied to the ecotoxicological testing of N. libonensis to two reference chemicals-3,5-dichlorophenol and potassium dichromate. A similar response of free and immobilized cells was observed between exposures to spiked stream water and synthetic medium, and through the conditions tested. This outcome suggests that N. libonensis may potentially provide reliable responses under direct in situ exposures.

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Sediment toxicity tests using benthic marine microalgae Cylindrotheca closterium (Ehremberg) Lewin and Reimann (Bacillariophyceae)

Cited by 38 publications

References 25 publications

Development and application of whole‐sediment toxicity test using immobilized freshwater microalgae Pseudokirchneriella subcapitata

Development and application of whole‐sediment toxicity test using immobilized freshwater microalgae Pseudokirchneriella subcapitata

Cadmium uptake from sediment by Cylindrotheca closterium and the effect of diatom presence on partitioning of cadmium between sediment and water: A laboratory study

Optimization of growth conditions for laboratory and field assessments using immobilized benthic diatoms

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