Sediment toxicity assessment using bacterial bioluminescence: effect of an unusual phytoplankton bloom

Bihari, Nevenka; Najdek, Mirjana; Floris, R.; Batel, Renato; Zahn, R. K.

doi:10.3354/meps057307

Cited by 12 publications

(9 citation statements)

References 5 publications

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“…18, 1999 189 sites in order to cover a wide range of sites known or suspected to be contaminated with polycyclic aromatic hydrocarbons (PAHs), polychlorinated biphenyls (PCBs), and pesticides. Ten samples (samples [5][6][7][8][9][10][11][12][13][14] were black in appearance, indicating a reduced depositional environment.…”

Section: Sample Collectionsupporting

confidence: 88%

“…One reason for this situation is that ecotoxicologic tests on sediment extracts can be subject to a variety of interferences [11][12][13] that may directly or indirectly affect the interpretation of test results. The potential for false positive toxicity results due to the presence of common macrocompounds (e.g., ammonia), from both natural and/or anthropogenic sources, may result in the erroneous conclusion that toxicity is due solely to the anthropogenic pollutants in the sample.…”

Section: Introductionmentioning

confidence: 99%

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Confirmation of elemental sulfur toxicity in the Microtox® assay during organic extracts assessment of freshwater sediments

Pardos

Benninghoff

Thomas

et al. 1999

Enviro Toxic and Chemistry

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Abstract-Recent literature indicates that the elemental sulfur occurring in organic extracts of sediment samples can be toxic to the bacterium Vibrio fischeri, used in standard Microtox bioassays. This observation was tested by means of the solvent extraction of 14 freshwater sediment samples from rivers tributary to Lake Geneva (Switzerland-France), measuring both Microtox toxicity and the elemental sulfur concentration of the extracts. Aliquots of these sediment extracts were further treated to remove the sulfur by adding acid-activated copper to the crude extracts; for 18 h in one case, and for 116 h in an other. The results were a significant amount of the observed acute toxicity in the Microtox assay of 81% of sample extracts (n ϭ 42, crude and after cleanup) was due to elemental sulfur, and despite a median decrease of 99.1% of elemental sulfur in the extracts subject to a 116-h cleanup, sulfur toxicity was not completely excluded for 57% (8/14) of the samples. Clearly, the Microtox methodology needs to be amended to more accurately assess the potential impact of organic pollutants in sediments when solvent extracts are used. This will help to cut down on costly and unnecessary remedial actions.

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Section: Sample Collectionsupporting

confidence: 88%

Section: Introductionmentioning

confidence: 99%

Confirmation of elemental sulfur toxicity in the Microtox® assay during organic extracts assessment of freshwater sediments

Pardos

Benninghoff

Thomas

et al. 1999

Enviro Toxic and Chemistry

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“…Therefore, the widely used Microtox bioassay, which provides a response to a single toxicant or to the combined effect of many toxicants when their concentration exceeds a threshold limit, was performed. The assay has been successfully applied for the detection of accidental toxic events or the determination of sediment toxicity (Bihari et al, 1989). In the Shuaiba coastal area the detected toxicity correlated with the combined effects of the pollutants present in sediment rather than with particular chemical parameters (Beg et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

“…The results are expressed as milliliters of seawater causing a 50% reduction in bioluminescence after 15 min (EC 50 ). Estimates of EC 50 were obtained using linear regression analysis, as described previously (Schiewe et al, 1985;Bihari et al, 1989). A reference toxicant, 4-nitroquinoline-N-oxide (2.5 g/mL), which reduces bacterial bioluminescence for 50%, was chosen as a positive control and tested each day to quantitate test variability.…”

Section: Methodsmentioning

confidence: 99%

Seawater quality along the Adriatic coast, Croatia, based on toxicity data

Bihari

Mičić

Fafanđel

2004

Environmental Toxicology

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The potential toxicity of organic extracts from 12 seawater samples from each of 24 sampling sites, collected during 1999-2001 along the Adriatic coast, Croatia, was analyzed with the Microtox toxicity bioassay. The results were consistent with the usefulness of Microtox for the detection of accidental toxic events. To determine the water quality of selected areas, cluster analysis for discrimination between groups with similar toxicity load and water quality index as a base for the ranking of sampling sites was introduced. Based on our experimental data, five classes of the quality index were defined, and so areas were ranked in five categories (excellent, good, fair, poor, and very poor) according to their potential toxic influence. The water quality of selected sites for the potential toxicity of organic extracts could be described as excellent at one sampling site, good at 15 sampling sites, and fair at eight sampling sites. Poor and very poor seawater quality was not detected.

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“…The acute toxicity of sediment extracts was investigated using a specially developed bioassay -Microtox®. (BIHARI et al, 1989 ;BIHARI et al, 2006;FERNANDEZ-PINAS et al, 2014). The toxicity of the sediment extracts was determined by the decrease in bioluminescence of Vibrio fisheri bacteria after exposure to sediment extracts.…”

Section: Acute Toxicity Of Sediment Extractsmentioning

confidence: 99%

Assessment of environmental risk related to the polycyclic aromatic hydrocarbons (PAH) in the sediments along the eastern Adriatic coast

Mandić

Veža

Kušpilić

2022

Acta Adriat. (Online)

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Polycyclic aromatic hydrocarbons (PAH) were investigated in the sediments at 24 locations in the coastal area of Adriatic Sea. Total PAH concentrations ranged from 14,98 to 26145,62 µg kg-1, with the highest levels determined in the sediments of Šibenik Bay and the lowest in the sediment near Island of Pag. Unsubstituted PAH were prevailing PAH group in the sediments from the Šibenik Bay and in the Coastal area, while methyl-substituted PAH were dominant group in the sediments of the Kaštela Bay. In order to estimate the possible toxicological significance of PAH concentrations, Sediment Quality Assessment was done according to three different sets of sediment quality guidelines. A high environmental risk was calculated for each station in Šibenik Bay; for the station in vicinity of the Industrial port of Split and for the two stations in Coastal zone. Low environmental risk was determined in less urbanised areas such as Island of Pag. Sediment extracts were further analysed for acute toxicity by measurements of decrease in bioluminescence upon exposure to sediment extracts. Obtained results show disagreement between sediment toxicity predicted upon PAH levels in sediment and experimentally obtained sediment toxicity. Sediment extracts in which low PAH levels were measured showed high acute toxicity indicating that concentrations of PAH alone are a poor indicator of sediment toxicity. Correlation analysis between concentrations of specific PAH compounds and toxicity revealed the strongest association between acute sediment toxicity and sulphur-containing PAH suggesting the S-PAH should be considered in sediment toxicity assessment.

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Sediment toxicity assessment using bacterial bioluminescence: effect of an unusual phytoplankton bloom

Cited by 12 publications

References 5 publications

Confirmation of elemental sulfur toxicity in the Microtox® assay during organic extracts assessment of freshwater sediments

Confirmation of elemental sulfur toxicity in the Microtox® assay during organic extracts assessment of freshwater sediments

Seawater quality along the Adriatic coast, Croatia, based on toxicity data

Assessment of environmental risk related to the polycyclic aromatic hydrocarbons (PAH) in the sediments along the eastern Adriatic coast

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