Sediment microbial community analysis: Establishing impacts of aquaculture on a tropical mangrove ecosystem

Castine, Sarah; Bourne, David G.; Trott, Lindsay; McKinnon, David

doi:10.1016/j.aquaculture.2009.09.013

Cited by 37 publications

(22 citation statements)

References 38 publications

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“…1A). This has been reported previously in mangroves using other molecular approaches [20], [21]. The dominant classes observed were Gammaproteobacteria and Deltaproteobacteria (Fig.…”

Section: Resultssupporting

confidence: 88%

Mangrove Bacterial Diversity and the Impact of Oil Contamination Revealed by Pyrosequencing: Bacterial Proxies for Oil Pollution

et al. 2011

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BackgroundMangroves are transitional coastal ecosystems in tropical and sub-tropical regions and represent biologically important and productive ecosystems. Despite their great ecological and economic importance, mangroves are often situated in areas of high anthropogenic influence, being exposed to pollutants, such as those released by oil spills.Methodology/Principal FindingsA microcosm experiment was conducted, which simulated an oil spill in previously pristine mangrove sediment. The effect of the oil spill on the extant microbial community was studied using direct pyrosequencing. Extensive bacterial diversity was observed in the pristine mangrove sediment, even after oil contamination. The number of different OTUs only detected in contaminated samples was significantly higher than the number of OTUs only detected in non-contaminated samples. The phylum Proteobacteria, in particular the classes Gammaproteobacteria and Deltaproteobacteria, were prevalent before and after the simulated oil spill. On the other hand, the order Chromatiales and the genus Haliea decreased upon exposure to 2 and 5% oil, these are proposed as sensitive indicators of oil contamination. Three other genera, Marinobacterium, Marinobacter and Cycloclasticus increased their prevalence when confronted with oil. These groups are possible targets for the biomonitoring of the impact of oil in mangrove settings.Conclusions/SignificanceWe suggest the use of sequences of the selected genera as proxies for oil pollution, using qPCR assessments. The quantification of these genera in distinct mangrove systems in relation to the local oil levels would permit the evaluation of the level of perturbance of mangroves, being useful in field monitoring. Considering the importance of mangroves to many other environments and the susceptibility of such areas to oil spills this manuscript will be of broad interest.

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“…1A). This has been reported previously in mangroves using other molecular approaches [20], [21]. The dominant classes observed were Gammaproteobacteria and Deltaproteobacteria (Fig.…”

Section: Resultssupporting

confidence: 88%

Mangrove Bacterial Diversity and the Impact of Oil Contamination Revealed by Pyrosequencing: Bacterial Proxies for Oil Pollution

et al. 2011

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“…1) agrees well with previous studies that have examined the response of benthic bacterial abundance to organic enrichment arising from aquaculture activities using direct counts 43–47 , fatty acid biomarkers 17 and molecular techniques 43–48 . By contrast, the relative abundances of other putative bacterial biomarker PLFAs, e.g.…”

Section: Discussionsupporting

confidence: 90%

Detecting the presence of fish farm-derived organic matter at the seafloor using stable isotope analysis of phospholipid fatty acids

Mayor

Gray

Hattich

et al. 2017

Sci Rep

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The expansion of global aquaculture activities is important for the wellbeing of future generations in terms of employment and food security. Rearing animals in open-exchange cages permits the release of organic wastes, some of which ultimately reaches the underlying sediments. The development of rapid, quantitative and objective monitoring techniques is therefore central to the environmentally sustainable growth of the aquaculture industry. Here, we demonstrate that fish farm-derived organic wastes can be readily detected at the seafloor by quantifying sediment phospholipid fatty acids (PLFAs) and their carbon stable isotope signatures. Observations across five farms reveal that farm size and/or distance away from it influence the spatial distribution of the generated organic wastes and their effect on benthic bacterial biomass. Comparison to the isotopic signatures of fish feed-derived PLFAs indicates that 16:0 and 18:1(n-9) are potential biomarkers for fish farm-derived organic wastes. Our results suggest that stable isotope analysis of sediment PLFAs has potential for monitoring the environmental performance of aquaculture activities, particularly given the increasing prevalence of terrigenous organic matter in aquaculture feed stocks because it is isotopically district to marine organic matter.

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“…Those studies showed that microbial community assembly was largely based on functions rather than phylogenetic similarity and could adapt to new environments by changing community structure and composition (Langenheder and Szekely, 2011;Liang et al, 2015;Logue and Lindstrom, 2010). In this study, our results showed that the most dominant phylum is Proteobacteria, including δ-Proteobacteria and γ-Proteobacteria, which exhibited higher abundance at CZ and generally function in sulfate-reduction, sulfuroxidization and nitrate assimilation (Allen et al, 2001;Asami et al, 2005;Castine et al, 2009;Kawahara et al, 2008). The second dominant phylum Bacteroidetes was in a higher proportion in water column of GZ, which is widely distributed in marine waters and has the potential of degrading biopolymers (Asami et al, 2005;Bissett et al, 2006).…”

Section: Certain Microbial Groups Enriched In Response To Seaweed Culmentioning

confidence: 59%

Large-scale seaweed cultivation diverges water and sediment microbial communities in the coast of Nan'ao Island, South China Sea

Xie

et al. 2017

Science of The Total Environment

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Sediment microbial community analysis: Establishing impacts of aquaculture on a tropical mangrove ecosystem

Cited by 37 publications

References 38 publications

Mangrove Bacterial Diversity and the Impact of Oil Contamination Revealed by Pyrosequencing: Bacterial Proxies for Oil Pollution

Mangrove Bacterial Diversity and the Impact of Oil Contamination Revealed by Pyrosequencing: Bacterial Proxies for Oil Pollution

Detecting the presence of fish farm-derived organic matter at the seafloor using stable isotope analysis of phospholipid fatty acids

Large-scale seaweed cultivation diverges water and sediment microbial communities in the coast of Nan'ao Island, South China Sea

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