Sediment-associated bacterial community and predictive functionalities are influenced by choice of 16S ribosomal RNA hypervariable region(s): An amplicon-based diversity study

Rajeev, Meora; Sushmitha, T.J.; Toleti, Subba Rao; Pandian, Shunmugiah Karutha

doi:10.1016/j.ygeno.2020.09.006

Cited by 10 publications

(1 citation statement)

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“…The primer pair selected here encompasses the V3–V4 hypervariable regions of 16S rRNA gene and has been frequently utilized in several 16S rRNA gene-based diversity studies 33 , 35 , as it offers a better analysis of bacterial diversity. The detailed protocol on amplicons library preparation has been discussed in our earlier studies 36 , 37 . The size and quality of prepared libraries were validated by running on Bioanalyzer 2100 (Agilent technologies, California, US) and taken in equal concentrations for paired-end (300 × 2 bp) sequencing on the Illumina MiSeq sequencing platform at Macrogen Inc. (Seoul, Korea).…”

Section: Methodsmentioning

confidence: 99%

Thermal discharge-induced seawater warming alters richness, community composition and interactions of bacterioplankton assemblages in a coastal ecosystem

Rajeev

Sushmitha

Aravindraja

et al. 2021

Sci Rep

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Despite accumulating evidence on the impact of global climate warming on marine microbes, how increasing seawater temperature influences the marine bacterioplankton communities is elusive. As temperature gradient created by thermal discharges provides a suitable in situ model to study the influence of warming on marine microorganisms, surface seawater were sampled consecutively for one year (September-2016 to August-2017) from the control (unimpacted) and thermal discharge-impacted areas of a coastal power plant, located in India. The bacterioplankton community differences between control (n = 16) and thermal discharge-impacted (n = 26) areas, as investigated using 16S rRNA gene tag sequencing revealed reduced richness and varied community composition at thermal discharge-impacted areas. The relative proportion of Proteobacteria was found to be higher (average ~ 15%) while, Bacteroidetes was lower (average ~ 10%) at thermal discharge-impacted areas. Intriguingly, thermal discharge-impacted areas were overrepresented by several potential pathogenic bacterial genera (e.g. Pseudomonas, Acinetobacter, Sulfitobacter, Vibrio) and other native marine genera (e.g. Marinobacter, Pseudoalteromonas, Alteromonas, Pseudidiomarina, Halomonas). Further, co-occurrence networks demonstrated that complexity and connectivity of networks were altered in warming condition. Altogether, results indicated that increasing temperature has a profound impact on marine bacterioplankton richness, community composition, and inter-species interactions. Our findings are immensely important in forecasting the consequences of future climate changes especially, ocean warming on marine microbiota.

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