Secretory phospholipase A2 and neonatal respiratory distress: pilot study on broncho-alveolar lavage

Luca, Danièle De; Baroni, Silvia; Vento, Giovanni; Piastra, Marco; Pietrini, Domenico; Romitelli, Federica; Capoluongo, Ettore; Romagnoli, Costantino; Conti, Giorgio; Zecca, Enrico

doi:10.1007/s00134-008-1224-3

Cited by 39 publications

(49 citation statements)

References 26 publications

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“…First, there are significant correlations between ELF sPLA2 activity, sPLA2-IIA concentration, TNFa and OI. This has not been observed in animal studies, but is consistent with the relationship between sPLA2 activity and gasexchange derangement in hyaline membrane disease and infection-related respiratory failure [11]. Similar findings were also found in paediatric and adult acute respiratory distress syndrome (ARDS) [12,38].…”

Section: Discussionsupporting

confidence: 74%

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Role of distinct phospholipases A2 and their modulators in meconium aspiration syndrome in human neonates

et al. 2011

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Section: Discussionsupporting

confidence: 74%

“…Therefore, phospholipases A2 are crucial in many types of adult and paediatric lung injury [10][11][12]. Meconium carries considerable amounts of pancreatic sPLA2 (subtype IB), bile acids and cytokines [3,13,14].…”

Section: Introductionmentioning

confidence: 99%

Role of distinct phospholipases A2 and their modulators in meconium aspiration syndrome in human neonates

et al. 2011

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“…TTN was clinically defined as the presence of tachypnea (respiratory rate > 60/min) and dyspnea (Silverman score > 1) appearing within the first 24 h of life, needing only oxygen supplementation and/or nasal continuous airway pressure (CPAP). Exclusion criteria were: (1) major malformations or chromosomal abnormalities; (2) early-onset sepsis or pneumonia (defined by the presence of clinical, radiological, and microbiological criteria as detailed elsewhere [8]) and increased inflammatory markers as per local NICU protocols, or the diagnosis of clinical chorioamnionitis (defined elsewhere [9]); (3) lack of parental consent; (4) a diagnosis of neonatal acute respiratory distress syndrome according to the Montreux definition [10]; (5) a diagnosis of classical hyaline membrane disease, i.e., respiratory distress syndrome (RDS) (as previously described [10]). Basically, RDS was defined as the presence of typical chest X-rays (a diffuse ground-glass appearance) and the need for surfactant replacement.…”

Section: Methodsmentioning

confidence: 99%

A Multicenter Lung Ultrasound Study on Transient Tachypnea of the Neonate

Raimondi

Yousef

Fanjul³

et al. 2019

Neonatology

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Background and Aim: Discordant results that demand clarification have been published on diagnostic lung ultrasound (LUS) signs of transient tachypnea of the neonate (TTN) in previous cross-sectional, single-center studies. This work was conducted to correlate clinical and imaging data in a longitudinal and multicenter fashion. Methods: Neonates with a gestational age of 34–40 weeks and presenting with TTN underwent a first LUS scan at 60–180 min of life. LUS scans were repeated every 6–12 h if signs of respiratory distress persisted. Images were qualitatively described and a LUS aeration score was calculated. Clinical data were collected during respiratory distress. Results: We enrolled 65 TTN patients. Thirty-one (47.6%) had a sharp echogenicity increase in the lower lung fields (the “double lung point” or DLP sign). On admission, there was no significant difference between patients with and without DLP in Silverman scores (4 ± 1.5 vs. 4 ± 2.1; p = 0.9) or LUS scores (7.6 ± 2.6 vs. 5.6 ± 3.8; p = 0.12); PaO₂/FiO₂ (249 ± 93 vs. 252 ± 125; p = 0.91). All initial LUS scans (performed at the onset of distress) and 99.5% of all scans showed a regular pleural line with no consolidation, with only 1 neonate showing consolidation in the follow-up scans. The Silverman and LUS scores were significantly correlated (rho = 0.27; p = 0.02). Conclusion: A regular pleural line with no consolidation is a consistent finding in TTN. The presence of a DLP is not essential for the LUS diagnosis of TTN. A semi-quantitative LUS score correlates well with the clinical course and could be useful in monitoring changes in lung aeration during TTN.

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“…Clara cell protein has been shown to produce its antiinflammatory action through the inhibition of phospholipase A 2 enzyme activity, by sparing surfactant phospholipids catabolism and preventing chronic lung disease, such as broncho-pulmonary dysplasia [11]. Interestingly, phospholipase A 2 seems to have the same effect both in the neonatal period and beyond this period as suggested by correlating levels of this enzyme with clinical severity, lung stiffness and need for respiratory support in neonates with iRDS [32] as well as with clinical severity, higher oxygen requirement and more aggressive ventilation in pediatric post-neonatal respiratory distress [33]. The fact that lower levels of cc-10 and a distinct pattern are present in this study may be of significance to the inflammatory process in iRDS since a decreased capacity to reduce lung inflammation will probably predispose these infants to further lung injury.…”

Section: Discussionmentioning

confidence: 84%

Clara Cell Protein Expression in Human Neonates During Respiratory Distress Syndrome

Arias-Martínez

Palacios-Sánchez

Delgado-Franco

et al. 2012

Cell Physiol Biochem

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Background/Aims: Clara cell protein (cc-10) has been shown to negatively regulate inflammation, protect pulmonary surfactant from degradation in the lung, and administration of this recombinant protein improves the condition of infant respiratory distress syndrome (iRDS), a disease that occurred mainly in preterm infants. In view of the possibility that altered expression of cc-10 might regulate its protective function, we attempted to characterize this protein in infants with iRDS. Methods: Using bronchotraqueal aspirates from human infants, we analyzed cc-10 in two-dimensional gel electrophoresis (2-DE) by combining immunoprecipitation, carbonyl groups and total protein immunoblotting. Results: Seven forms of cc-10 were detected with western immunoblots in infants with iRDS while only four forms were present in neonates who needed mechanical ventilation for other reasons without any lung disease (control group). The overall levels of cc-10 in iRDS were lower and differences were seen in isoform pattern and distribution. Conclusion: Our demonstration that cc-10 is differentially expressed in infants with iRDS may point the way towards one possible mechanism that potentially involves modifications of the protein structure with its anti-inflammatory and surfactant protective function and could be detrimental for this airway disorder.

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Secretory phospholipase A2 and neonatal respiratory distress: pilot study on broncho-alveolar lavage

Abstract: These are the first data about phospholipase A2 in neonates. The enzyme plays a role in neonatal lung injury, especially in infection related respiratory failure. It is associated with lung stiffness, higher mean airway pressure and need for oxygen.

Cited by 39 publications

References 26 publications

Role of distinct phospholipases A2 and their modulators in meconium aspiration syndrome in human neonates

Role of distinct phospholipases A2 and their modulators in meconium aspiration syndrome in human neonates

A Multicenter Lung Ultrasound Study on Transient Tachypnea of the Neonate

Clara Cell Protein Expression in Human Neonates During Respiratory Distress Syndrome

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