Secretory Patterns of Atrial Natriuretic Factor (ANF) By Culture Cardiocytes of Right and Left Atrium From Newborn and Adult RS

Cantin, M.; Dagenais, Nigel J.; Salmi, L; Gutkowska, Jolanta; Ballak, M.; Thibault, Gaétan; García, Raúl; Genest, Jacques

doi:10.3109/10641968509077221

Cited by 13 publications

(2 citation statements)

References 26 publications

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“…Iwai and Inagami (7) and Kakar et al (13) have proposed that AT lb-R mediates endocrine function such as the secretion of aldosterone from the adrenal gland, and adrenocorticotropic hormone and prolactin from the pituitary gland, because of its localized distribution in the rat tissue. The cardiomyocytes secrete atrial natriuretic factor (ANF), whereas ANF granules are not present in cardiac fibroblasts (43,44). It has been reported that AngII directly stimulates ANF secretion from neonatal rat cardiomyocytes, but not from cardiac fibroblasts (43).…”

Section: Discussionmentioning

confidence: 99%

Differential gene expression and regulation of angiotensin II receptor subtypes in rat cardiac fibroblasts and cardiomyocytes in culture.

Matsubara¹,

Kanasaki²,

Murasawa³

et al. 1994

J. Clin. Invest.

142

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Although both rat cardiac nonmyocytes (mostly fibroblasts) and cardiomyocytes have a functional angiotensin II (AngII) receptor, the regulation mechanism of its subtype expression in the rat heart remains unknown. In this study, by using a binding assay and a competitive reverse-transcriptase polymerase chain reaction, we examined the regulation of AngII types la and lb (ATla-R and AT1b-R) and type 2 receptor (AT2-R) expression in embryonal day 19 (E19) and neonatal (1-d) rat cardiac fibroblasts and cardiomyocytes. The number of AT2-R in E19 fibroblasts was dramatically decreased (from 305 to 41 fmol/mg protein) in 1-d fibroblasts, whereas that of AT1-R and the mRNA levels remained unchanged. The ratio of ATla-R to AT1b-R mRNA in both E19 and 1.4 fibroblasts was 9:1. The number of AT2-R in E19 cardiomyocytes was also significantly decreased (from 178 to 87 fmol/mg protein) in 1-d cardiomyocytes, whereas the magnitude was less prominent compared with that in fibroblasts. AT1-R expression remained unaltered in E19 and 1-d cardiomyocytes. In E19 and l-d cardiomyocytes, the ATlb-R mRNA level was 1.5-fold higher than that of AT1a-R mRNA. Dexamethasone induced significant increases in AT1a-R mRNA (2.1-fold) and numbers (1.8-fold) without changing the affinity, whereas neither AT1b-R mRNA nor the number of AT2-R was affected by dexamethasone. The ATla-R gene transcription rate, determined by means of a nuclear run-off assay, was increased (2-fold) by dexamethasone. The half-life of ATla-R mRNA (18 h) was unchanged by dexamethasone. These data indicate that AngI1 receptor subtype expression in the rat heart is regulated in a cell-and subtype-specific manner. (J. Clin. Invest. 1994.

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Section: Discussionmentioning

confidence: 99%

Differential gene expression and regulation of angiotensin II receptor subtypes in rat cardiac fibroblasts and cardiomyocytes in culture.

Matsubara¹,

Kanasaki²,

Murasawa³

et al. 1994

J. Clin. Invest.

142

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“…Furthermore, the use of cRNA probes has been shown to give hybridization of higher specificity and sensitivity (35,36) than that obtained with other probe types. Rat atrial cultures have previously been used to investigate the biosynthesis and secretion of prepro-ANP (13,37,38). The present findings indicate the potential value of using cRNA probes and cardiac cultures to examine the regulation of ANP gene expression in atrial and nonatrial tissues.…”

Section: Discussionmentioning

confidence: 57%

Localization of atrial natriuretic peptide mRNA and immunoreactivity in the rat heart and human atrial appendage.

Hamid

Wharton

Terenghi

et al. 1987

Proc. Natl. Acad. Sci. U.S.A.

131

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The localization of mRNA encoding preproatrial natriuretic peptide was investigated in tissue sections and cultures of rat heart and in sections of human right atrial appendage using the technique of in situ hybridization with 32P-and 35S-labeled RNA probes. Rat atrial natriuretic peptide (ANP) transcripts were demonstrated in numerous atrial myocytes and, to a lesser extent, in ventricular myocytes in both tissue sections and newborn rat heart cultures. These rmdings are consistent with those obtained by RNA blot analysis of rat heart total RNA, indicating that a single prepro-ANP transcript of ""900 nucleotides was present in the ventricles as well as the atria. Using a 35S-labeled RNA probe for human ANP mRNA, ANP transcripts were also localized to the majority of myocytes in the human right atrial appendage. Only background levels of autoradiographic labeling were obtained when RNA probes identical to the coding sequence of rat or human ANP mRNA were used. A close correlation was found between the distribution of ANP immunoreactivity and prepro-ANP mRNA in these preparations. These results provide unequivocal evidence for the expression of the ANP gene in the rat ventricles, as well as the atria, because myocytes in these tissues have been established as the sites of both ANP localization and precursor biosynthesis. The combined use of cardiac cultures and in situ hybridization may be of value in future studies investigating the regulation of ANP synthesis in cardiac myocytes.Cloning and sequence analysis of the complementary DNA (cDNA) encoding rat and human atrial natriuretic peptide (ANP) mRNA has revealed the structure of the prepro-ANP molecule, which consists of 151 and 152 amino acids in the human and rat, respectively (1-5). The nucleotide sequence of the genomic DNA encoding prepro-ANP has also been determined (6-11) and in man the gene is known to consist of 2 kilobase pairs, comprising three exons and two introns, located on the distal short arm of chromosome 1 (12). The ANP gene is expressed mainly in atrial tissues that contain both ANP transcripts (6, 13-16) and ANP immunoreactivity, the latter being localized to secretory granules in the atrial myocytes (17-19).However, in nonmammalian species ANP-like immunoreactivity occurs in the ventricles as well as the atria (18), and the results of mRNA blot analysis (6, 16) and radioimmunoassay (15, 19) together indicate at least some expression of the ANP gene in the ventricles of the rat, although normally at a lower level than in the atria. While the presence of atrial natriuretic peptides in ventricular cells might result from their synthesis at these sites, the possible uptake of circulating ANP cannot be excluded (15). Furthermore, immunocytochemical techniques alone are not very useful for examining the regulation of prepro-ANP production because it is not possible to distinguish between changes in synthesis and secretion. In situ hybridization provides a method for demonstrating gene expression at the cellular level and may be of value ...

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Ventricular and atrial myocytes of newborn rats synthesise and secrete atrial natriuretic peptide in culture: Light-and electron-microscopical localisation and chromatographic examination of stored and secreted molecular forms

et al. 1988

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We have demonstrated that atrial natriuretic peptide-like immunoreactivity is stored and secreted by ventricular and atrial myocytes in dissociated cell culture preparations from the heart of newborn rat. Culture preparations were maintained in either foetal calf serum-supplemented medium 199 or in hormone-supplemented, serum-free medium 199. The presence of atrial natriuretic peptide-like immunoreactivity in the cultured myocytes was demonstrated at both light- and electron-microscopical levels. Release of atrial natriuretic peptide-like immunoreactivity into the culture medium was measured by radioimmunoassay; molecular forms of the stored and secreted peptide were determined by gel column chromatography. The atrial natriuretic peptide-like immunoreactivity of cultured atrial and ventricular myocytes was concentrated in the perinuclear cytoplasm and was localised to electron-dense secretory granules. The number of immunoreactive ventricular myocytes and the intensity of their immunofluorescence changed with time in culture and was higher in cultures in foetal calf serum-supplemented medium than in serum-free medium. Gamma-atrial natriuretic peptide was stored and released by cultured atrial and ventricular myocytes, but was broken down to alpha-atrial natriuretic peptide in the growth medium. This process was foetal calf serum-independent, since it occurred in both the media used, indicating that cardiac myocytes in culture may release a factor that cleaves gamma-atrial natriuretic peptide to form alpha-atrial natriuretic peptide.

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Secretory Patterns of Atrial Natriuretic Factor (ANF) By Culture Cardiocytes of Right and Left Atrium From Newborn and Adult RS

Cited by 13 publications

References 26 publications

Differential gene expression and regulation of angiotensin II receptor subtypes in rat cardiac fibroblasts and cardiomyocytes in culture.

Differential gene expression and regulation of angiotensin II receptor subtypes in rat cardiac fibroblasts and cardiomyocytes in culture.

Localization of atrial natriuretic peptide mRNA and immunoreactivity in the rat heart and human atrial appendage.

Ventricular and atrial myocytes of newborn rats synthesise and secrete atrial natriuretic peptide in culture: Light-and electron-microscopical localisation and chromatographic examination of stored and secreted molecular forms

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