Secretory Leukocyte Protease Inhibitor (SLPI) Increases Focal Adhesion in MC3T3 Osteoblast on Titanium Surface

Abstract

An appropriate interaction between implanted materials and the surrounding tissue is essential for successful implantation. Titanium (Ti) and some of its alloys have been used in dentistry and orthopedics as a substitutive material for hard tissue, such as teeth or natural bone. Nevertheless, metal ions released from titanium and alloy implants have adverse biological effects on biological tissues or cells. Secretory leukocyte protease inhibitor (SLPI) promotes cell migration, proliferation and wound healing. FAK and ERK1/2 signaling regulate cell adhesion and proliferation for cell survival. This study evaluated the potential of SLPI as a molecule to increase the cell adhesion on the Ti surface. Compared with the untreated cells, SLPI increased the adhesion of MC3T3-E1 cells to Ti discs, formation of actin stress fibers, paxillin expression and the phosphorylation of FAK. Moreover, SLPI enhanced the level of Grb2 and Ras expression and ERK1/2 phosphorylation in the MC3T3-E1 cells on Ti discs. These results suggest that SLPI can increase the interaction between the implanted Ti material and surrounding bone in orthodontic and dental surgery, making an effective nanomolecule for successful implantation.

Secretory leukocyte protease inhibitor promotes differentiation and mineralization of MC3T3-E1 preosteoblasts on a titanium surface

“…A comparison of the present results with other studies indicates that SLPI can promote the differentiation and mineralization of MC3T3-E1 cells on a Ti surface. In previous studies, SLPI was shown to increase MC3T3-E1 cell adhesion to a Ti-surface as well as the proliferation of KB human oral carcinoma cells (9,13). In addition, SLPI was reported to increase mineralized nodule formation and expression of BSP, DSPP, osteocalsin, osteonectin and Col I, which are associated with odontoblast differentiation and mineralization, thereby acting as a stimulant of these processes (19).…”

“…Commercially pure titanium (Cp-Ti) discs were kindly provided by Professor Han-Cheol Choe (Department of Dental Materials, School of Dentistry, Chosun University, Gwangju, Republic of Korea). Polished Cp-Ti discs were prepared using a method described previously (9).…”

“…The MC3T3-E1 cells plated on the Ti discs (6x10 5 cells/ml) were incubated for 4, 7 and 10 days in differentiation medium with or without rhSLPI. An MTT assay (Sigma-Aldrich, St. Louis, MO, USA) was performed to examine the cell viability as described previously (9).…”

“…SLPI acts as an anti-inflammatory factor in the early inflammatory response of odontoblasts (8). A recent study reported that SLPI accelerates the adhesion and migration of MC3T3-E1 cells on Ti surfaces by increasing the formation of actin stress fibers, paxillin expression and focal adhesion kinase phosphorylation (9). Conversely, there are no studies regarding the effect of SLPI on the differentiation and mineralization of osteoblasts on a Ti surface.…”

Secretory leukocyte protease inhibitor reduces inflammation and alveolar bone resorption in LPS-induced periodontitis in rats and in MC3T3-E1 preosteoblasts

“…Highly expressed SLPI in LED-radiated RAW264.7 cells inhibits the expression of IL-1β and TNF-α (Jeong et al 2015a). Furthermore, recent studies have reported that SLPI regulates the formation of the dentin matrix and mineralization in odontoblasts, and increases adhesion and viability of osteoblasts on titanium surfaces (Jeong et al 2015b(Jeong et al , 2015c. Based on previous studies, SLPI may reduce the inflamed periodontal tissue and bone resorption.…”