Secretory-IgA binding to intestinal microbiota attenuates inflammatory reactions as the intestinal barrier of preterm infants matures

Abstract

Previous work has shown that Secretory-IgA (SIgA) binding to the intestinal microbiota is variable and may regulate host inflammatory bowel responses. Nevertheless, the impact of the SIgA functional binding to the microbiota remains largely unknown in preterm infants whose immature epithelial barriers make them particularly susceptible to inflammation. Here, we investigated SIgA binding to intestinal microbiota isolated from stools of preterm infants <33 wk gestation with various levels of intestinal permeability. We found that SIgA binding to intestinal microbiota attenuates inflammatory reactions in preterm infants. We also observed a significant correlation between SIgA affinity to the microbiota and the infant’s intestinal barrier maturation. Still, SIgA affinity was not associated with developing host defenses, such as the production of mucus and inflammatory calprotectin protein, but it depended on the microbiota shifts as the intestinal barrier matures. In conclusion, we reported an association between the SIgA functional binding to the microbiota and the maturity of the preterm infant’s intestinal barrier, indicating that the pattern of SIgA coating is altered as the intestinal barrier matures.

Early host immune responses in a human organoid-derived gallbladder monolayer to Salmonella Typhi strains from patients with acute and chronic infections: a comparative analysis

“…To partially address this shortcoming, we used a HODGM model developed by our group ( Figure 1A ). Gallbladder stem cell (SC) isolation, culture, and establishment of the HODGM model were performed according to previous protocols with few modifications ( 29 – 31 ). Briefly, gallbladder tissues were rinsed with chelation buffer (2% sorbitol, 1% D-sucrose, 1% bovine serum albumin fraction V (BSA), 10 μg/ml Gentamicin and 250 ng/ml Amphotericin in Dulbecco’s Phosphate buffered saline) (Sigma-Aldrich-Aldrich, St Louis, MO, USA)), minced, and incubated with PBS/2 mM EDTA for 30 minutes to release SC clusters.…”

“…To partially address this shortcoming, we used a HODGM model developed by our group (Figure 1A). Gallbladder stem cell (SC) isolation, culture, and establishment of the HODGM model were performed according to previous protocols with few modifications (29)(30)(31). Briefly, gallbladder tissues were rinsed with chelation buffer…”

Dietary fiber pectin: challenges and potential anti-inflammatory benefits for preterms and newborns

“…However, the presence of SIgA, a predominant immunoglobulin in the human gut, relies on the gastrointestinal tract's colonization by microbiota. SIgA is critical in attenuating inflammatory reactions in the intestine, particularly in preterm infants (37)(38)(39)(40). For example, germ-free mice, lacking microbiota, demonstrate a marked reduction of SIgA in the gut (41)(42)(43).…”

Intestinal Barrier Dysfunction and Gut Microbiota in Non-Alcoholic Fatty Liver Disease: Assessment, Mechanisms, and Therapeutic Considerations

“…IgE is essential in the interaction between antigens and intestinal mucosal absorption and is a critical factor in intestinal allergic reactions. Furthermore, IgM plays a protective function in the intestinal mucosa and contributes to the development of inflammatory responses and tissue damage in specific gastrointestinal disorders [38].…”