Secretory dynamics of the rat submandibular gland. An ultrastructural and cytochemical study of the isoproterenol-induced secretory cycle

Bogart, Bruce Ian

doi:10.1016/s0022-5320(75)80107-9

Cited by 68 publications

(18 citation statements)

References 62 publications

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“…Many similarities between this p-nitrophenylphosphatase and Na-K-ATPase were well documented (4, 21, 29, 51, 61), and it is well established that K-NPPase is a part of Na-K-ATPase complex. The method by Ernst has so far been applied to the studies of the Na-K-ATPase pumps in several other tissues (7,12,13,37,64,76), and, undoubtedly, seems to be the most widely accepted approach for localizing Na-K-ATPase activity. However, his method has two fundamental drawbacks.…”

Section: Discussionmentioning

confidence: 99%

ULTRACYTOCHEMICAL LOCALIZATION OF OUABAIN-SENSITIVE, POTASSIUM-DEPENDENT <i>P</i>-NITROPHENYLPHOSPHATASE ACTIVITY IN THE GUINEA PIG RETINA

Ueno

Mayahara

Tsukahara

et al. 1980

Acta Histochem. Cytochem

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Section: Discussionmentioning

confidence: 99%

ULTRACYTOCHEMICAL LOCALIZATION OF OUABAIN-SENSITIVE, POTASSIUM-DEPENDENT <i>P</i>-NITROPHENYLPHOSPHATASE ACTIVITY IN THE GUINEA PIG RETINA

Ueno

Mayahara

Tsukahara

et al. 1980

Acta Histochem. Cytochem

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“…These vacuoles may result from either an expansion of the existing dense bodies due to an increase in autophagic activity or to a swelling of the existing vacuoles. Autophagia accompanies many physiologic and pathologic phenomena, including remodeling of the cells during differentiation, involution, and metamorphosis (18); reorganization of the cell during hypersecretion (5,19); stimulation of the liver cell by glucagon (13); and many experimental examples of sublethal and lethal cell injury (18,42,43). However, the increase in the autophagic vacuoles and other secondary lysosomes may result in a swelling of these structures.…”

Section: Discussionmentioning

confidence: 99%

The Biologic Activities of Cystic Fibrosis Serum. II. Ultrastructural Aspects of the Effect of Cystic Fibrosis Sera and Calcium Ionophore A23187 on Rabbit Tracheal Explants

et al. 1978

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“…The existence of Na +/K + ATPase was reported in the rat mandibular gland (SCHWARTZ and MOORE, 1968;BOGART, 1975), and there are many reports on the inhibitory effects by ouabain on salivary secretion (PETERSEN and PoULSEN, 1967;MARTINEZ, 1971;MARTINEZ and CASSITY, 1983) and on Na+ dependency of salivary secretion (PETERSEN, 1970;CASE and HUNTER, 1982). BURGEN (1956) andIMAI (1965) reported that ACh increased the Na + entry in the salivary gland.…”

Section: Discussionmentioning

confidence: 99%

31P NMR studies on the isolated perfused mandibular gland of the rat.

et al. 1987

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Phosphorus nuclear magnetic resonance (31P NMR) was used to study energy metabolism in the rat mandibular gland. The gland was isolated, perfused arterially and set in the NMR tube. At rest, 7 resonance peaks were observed and 6 peaks identified from low field as: 1) sugar phosphates (SP) and nucleotide monophosphate (NMP), 2) inorganic phosphate (Pi), 3) creatine phosphate (PCr), 4) y-nucleotide triphosphate (NTP) and f3-nucleotide diphosphate (NDP), 5) a-NTP, a-NDP, NAD+, and NADH, 6) an unknown peak, and 7) 13-NTP. From the results of high performance liquid chromatography (HPLC), NTP consisted mainly of ATP and GTP, and UTP was not detected. The tissue contents of ATP and GTP in the perfused gland were determined by HPLC as 1.86 + 0.03 and 0.37 + 0.01 mmol/kg wet tissue (S.E., n = 5). From 31P NMR and HPLC data, the tissue levels of creatine phosphate, ADP, and sugar phosphates were estimated as 3.3, 0.4, and 4.2 mmol/kg wet tissue, respectively. The cessation of perfusion decreased the tissue levels of PCr and ATP and increased those of Pi and SP. On the other hand, administration of acetylcholine (1 ELM), which is an optimal dose for secretion, decreased PCr and increased Pi but did not change SP. The ATP was unchanged initially and slowly decreased to the lower level during sustained secretion. These findings suggest that a sustained secretion requires more energy from ATP hydrolysis rather than initial secretion.

show abstract

Secretory dynamics of the rat submandibular gland. An ultrastructural and cytochemical study of the isoproterenol-induced secretory cycle

Cited by 68 publications

References 62 publications

ULTRACYTOCHEMICAL LOCALIZATION OF OUABAIN-SENSITIVE, POTASSIUM-DEPENDENT <i>P</i>-NITROPHENYLPHOSPHATASE ACTIVITY IN THE GUINEA PIG RETINA

ULTRACYTOCHEMICAL LOCALIZATION OF OUABAIN-SENSITIVE, POTASSIUM-DEPENDENT <i>P</i>-NITROPHENYLPHOSPHATASE ACTIVITY IN THE GUINEA PIG RETINA

The Biologic Activities of Cystic Fibrosis Serum. II. Ultrastructural Aspects of the Effect of Cystic Fibrosis Sera and Calcium Ionophore A23187 on Rabbit Tracheal Explants

31P NMR studies on the isolated perfused mandibular gland of the rat.

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