Secretory and cytosolic phospholipase A2 activities and expression are regulated by oxytocin and estradiol during labor

Farina, Mariana; Billi, Silvia; Leguizamón, Gustavo; Weissmann, Carina; Guadagnoli, Tamara; Ribeiro, Maria L.C.; Franchi, A.M.

doi:10.1530/rep-07-0078

Cited by 23 publications

(19 citation statements)

References 46 publications

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“…An explanation may be linked to a possible influence of hormonal status on sPLA2-IIa activity. In fact, several studies evidenced that PLA2 activity is modulated by both agonists and antagonists of steroid hormones (e.g., 17b-estradiol, progesterone, and tamoxifen), in breast as well as in other tissues [45][46][47][48]. Another possible mechanism may be related to the significant expression of Annexin II (an endogeneous inhibitor of PLA2 activity) in breast cancer tissues respect to the undetectable levels in normal and hyperplastic breast ductal epithelial cells [49]; the dysregulated balance between sPLA2-IIa activity and its inhibition by annexins has been proposed in the pathogenesis of prostate cancer [50].…”

Section: Discussionmentioning

confidence: 99%

Nipple aspirate fluids from women with breast cancer contain increased levels of group IIa secretory phospholipase A2

Mannello

Qin

Zhu

et al. 2007

Breast Cancer Res Treat

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Arachidonic acid, a bioactive molecule metabolized into prostaglandins and leukotrienes, contributes to cellular proliferation and tumor progression. Group IIa secretory phospholipase A2 (sPLA2-IIa) can facilitate arachidonate release from cellular phospholipids, suggesting its involvement in tumor evolution. Analysis of breast nipple aspirate fluid (NAF), a noninvasive research tool, allows the identification of biomarkers of breast cancer. We sought to determine whether sPLA2-IIa expression might be related to breast cancer development or progression. sPLA2-IIa expression was evaluated in NAF samples from 110 women (57 women with and 53 without breast cancer) using ELISA and western blotting; ultrastructural immunolocalization was performed in epithelial cells floating in NAF. Immunocytochemistry revealed that sPLA2-IIa is a constitutive intracellular protein suggesting that breast ductal cells synthesize and secrete the 14 kDa protein in NAF. Among all 110 subjects, sPLA2-IIa expression was significantly increased both in NAF and within ductal epithelial cells from cancer containing breasts. While in healthy women menopausal status did not influence sPLA2-IIa expression (P = 0.457), among patients with breast cancer there was a significant down-regulation in postmenopausal subjects (P < 0.0001). Moreover, sPLA2-IIa concentration in NAF from breast cancer patients was positively correlated with tumor stage (r (2) = 0.979, P = 0.0012), suggesting an active secretion/accumulation of the enzyme in NAF based on tumor burden. sPLA2-IIa activity may serve a dual role in breast carcinogenesis, beneficial in its release of arachidonate and detrimental in the metabolic conversion of arachadonic acid into prostaglandins and leukotrienes.

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Section: Discussionmentioning

confidence: 99%

Nipple aspirate fluids from women with breast cancer contain increased levels of group IIa secretory phospholipase A2

Mannello

Qin

Zhu

et al. 2007

Breast Cancer Res Treat

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“…Indeed, in pregnant rats uterine Ca 2? -PLA 2 activity is elevated near gestation in response to increased estradiol concentrations [156]. Estradiol-administration also enhanced the activity of Ca 2?…”

Section: Increased Concentration Of Intracellular Ppara Ligandsmentioning

confidence: 93%

Elevated Production of Docosahexaenoic Acid in Females: Potential Molecular Mechanisms

Kitson

Stroud

Stark

2010

Lipids

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Observational evidence suggests that in populations consuming low levels of n-3 highly unsaturated fatty acids, women have higher blood levels of docosahexaenoic acid (DHA; 22:3n-6) as compared with men. Increased conversion of alpha-linolenic acid (ALA; 18:3n-3) to DHA by females has been confirmed in fatty acid stable isotope studies. This difference in conversion appears to be associated with estrogen and some evidence indicates that the expression of enzymes involved in synthesis of DHA from ALA, including desaturases and elongases, is elevated in females. An estrogen-associated effect may be mediated by peroxisome proliferator activated receptor-alpha (PPARalpha), as activation of this nuclear receptor increases the expression of these enzymes. However, because estrogens are weak ligands for PPARalpha, estrogen-mediated increases in PPARalpha activity likely occur through an indirect mechanism involving membrane-bound estrogen receptors and estrogen-sensitive G-proteins. The protein kinases activated by these receptors phosphorylate and increase the activity of PPARalpha, as well as phospholipase A(2) and cyclooxygenase 2 that increase the intracellular concentration of PPARalpha ligands. This review will outline current knowledge regarding elevated DHA production in females, as well as highlight interactions between estrogen signaling and PPARalpha activity that may mediate this effect.

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“…Parent (1), fue de los primeros investigadores en reportar que la suplementación de OT a CEEP, induce la síntesis y liberación de PGF 2 α en mayor cantidad que PGE 2 hasta las 24 horas de cultivo (19), estudios posteriores de Woclawek (27) lo han corroborado. No obstante, en este trabajo la OT no tuvo efecto sobre la PGF 2 α pero si indujo un aumento en la concentración de PGE 2 , en las primeras 24 horas de cultivo (PGF 2 α< PGE 2 ); este resultado también ha sido reportado por Kotani (28), en cultivo de CEEP humanas y se explica por la presencia en la superficie celular de la enzima oxitocinasa que metaboliza la OT inhibiendo su ruta de acción y favoreciendo la síntesis de PGE 2 (29). Sin embargo, a las 36 horas se presentó un cambió en la concentración y proporción de la PGF 2 α en el sobrenadante (PGF 2 α> PGE 2 ).…”

Section: Discussionunclassified

Efecto del ácido linoléico sobre la producción de las prostaglandinas PGF2α y PGE2 en células endometriales

2013

Rev MVZ Córdoba

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RESUMENObjetivo. Evaluar el efecto in vitro del ácido linoléico sobre la producción de PGF 2 α y PGE 2 en células endometriales epiteliales bovinas (CEEP). Materiales y métodos. Se cultivaron CEEP aisladas de tejido uterino y se suplementaron con AL a diferentes concentraciones (1 μM, 10 μM, 100 μM), oxitocina (OT) (0.1 μM) e interferón trofoectodérmico bovino (bINT-τ) (50 ng/ml). Se cuantificó la PGF 2 α y PGE 2 a distintos tiempos (12, 24 y 36h). En el control, la PGF 2 α en el sobrenadante aumentó en el tiempo de cultivo y fue 1.2 veces mayor que la producción de PGE 2 . Resultados. El ácido linoléico disminuyó la concentración de PGF 2 α (p<0.05) en el sobrenadante, y no afectó (p>0.05) la producción de PGE 2 . El efecto conjunto de AL y OT sobre la producción de PGF 2 α difirió para cada uno de los tiempos; el ácido linoléico inhibió parcialmente el efecto estimulante de la OT sobre la producción de PGE 2 , el efecto conjunto del AL y el bINT-τ aumentó (p<0.05) esta inhibición hasta la hora 24. Conclusiones. El ácido linoléico afecta negativamente la concentración de PGF 2 α en el sobrenadante a través del tiempo. Respecto a la PGE 2 se concluye que el ácido linoléico por sí solo no afecta la concentración en el sobrenadante.Palabras clave: Endometrio, interferones, luteólisis, maternal, oxitocina (Fuente: MeSH) ABSTRACT Objective: Evaluate the in vitro effect of AL on the production of PGF 2 a and PGE 2 in bovine epithelial endometrial cells (CEEP). Materials and methods. Isolated CEEP were cultured and supplemented with AL at different concentrations (1μM, 10μM, 100μM), oxytocin (OT) (0.1 μM) and bovine interferon (BINT-τ) (50 ng / ml). PGF 2 a and PGE 2 were quantified as response variables at different times. In the control, the concentration of PGF 2 a in the supernatant increased over time and was 1.2 times greater than the production of PGE 2 . Results. AL reduced the concentration of PGF 2 a (p <0.05) in the supernatant, and did not affect (p> 0.05) the production of PGE 2 . The combined effect of LA and OT in the production of PGF 2 a is different in time, LA partially inhibited the stimulatory effect of OT in the production of PGE 2 , the joint effect of LA and BINT-τ increased (p<0.05) when this inhibition at 24h. Conclusions. LA alone negatively affects the concentration in the supernatant of PGE 2 in time. Regarding PGE 2 , LA alone does not affect the concentration in the supernatant.

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Secretory and cytosolic phospholipase A2 activities and expression are regulated by oxytocin and estradiol during labor

Cited by 23 publications

References 46 publications

Nipple aspirate fluids from women with breast cancer contain increased levels of group IIa secretory phospholipase A2

Nipple aspirate fluids from women with breast cancer contain increased levels of group IIa secretory phospholipase A2

Elevated Production of Docosahexaenoic Acid in Females: Potential Molecular Mechanisms

Efecto del ácido linoléico sobre la producción de las prostaglandinas PGF2α y PGE2 en células endometriales

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