Secretory Activity of Mesenchymal Stromal Cells with Different Degree of Commitment under Conditions of Simulated Microgravity

Zhivodernikov, I. V.; Ratushnyy, A. Yu.; Buravkova, Ludmila

doi:10.1007/s10517-021-05106-6

Cited by 4 publications

(3 citation statements)

References 13 publications

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“…IL-8 is one of the most important components of the MSC secretome (IL-8). It is a pro-inflammatory cytokine with its main function being to attract neutrophils and macrophages to inflammation foci [ 52 ]. In addition, IL-8 is involved in the cellular aging processes [ 53 ], and it is able to stimulate VEGF production, therefore exhibiting proangiogenic properties [ 52 , 54 , 55 ].…”

Section: Resultsmentioning

confidence: 99%

Specifics of Cryopreservation of Hydrogel Biopolymer Scaffolds with Encapsulated Mesenchymal Stem Cells

Egorikhina,

Rubtsova,

Linkova

et al. 2024

Polymers

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The demand for regenerative medicine products is growing rapidly in clinical practice. Unfortunately, their use has certain limitations. One of these, which significantly constrains the widespread distribution and commercialization of such materials, is their short life span. For products containing suspensions of cells, this issue can be solved by using cryopreservation. However, this approach is rarely used for multicomponent tissue-engineered products due to the complexity of selecting appropriate cryopreservation protocols and the lack of established criteria for assessing the quality of such products once defrosted. Our research is aimed at developing a cryopreservation protocol for an original hydrogel scaffold with encapsulated MSCs and developing a set of criteria for assessing the quality of their functional activity in vitro. The scaffolds were frozen using two alternative types of cryocontainers and stored at either −40 °C or −80 °C. After cryopreservation, the external state of the scaffolds was evaluated in addition to recording the cell viability, visible changes during subsequent cultivation, and any alterations in proliferative and secretory activity. These observations were compared to those of scaffolds cultivated without cryopreservation. It was shown that cryopreservation at −80 °C in an appropriate type of cryocontainer was optimal for the hydrogels/adipose-derived stem cells (ASCs) tested if it provided a smooth temperature decrease during freezing over a period of at least three hours until the target values of the cryopreservation temperature regimen were reached. It was shown that evaluating a set of indicators, including the viability, the morphology, and the proliferative and secretory activity of the cells, enables the characterization of the quality of a tissue-engineered construct after its withdrawal from cryopreservation, as well as indicating the effectiveness of the cryopreservation protocol.

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Section: Resultsmentioning

confidence: 99%

Specifics of Cryopreservation of Hydrogel Biopolymer Scaffolds with Encapsulated Mesenchymal Stem Cells

Egorikhina,

Rubtsova,

Linkova

et al. 2024

Polymers

View full text Add to dashboard Cite

show abstract

“…The decreasing Wnt also reduced OPG concentration. The study also revealed that IL-8 secreted by osteoblast committed MSCs, a chemoattractant of neutrophils and macrophages promoting their migration to the inflammation foci, upregulated in microgravity environment [18]. The increasing IL-8 modulated by NF-κB pathway was shown to accelerate bone resorption [20].…”

Section: Nf-κb Pathwaymentioning

confidence: 93%

“…Osteoprotegrin (OPG) is another significant element secreted by osteoblasts. OPG tends to bind to RANKL as well and it competes with RANK [18]. Wnt expressed by osteoroprogenitor cells which also promote osteoblast maturation upregulates OPG and develop osteoblast differentiation.…”

Section: Nf-κb Pathwaymentioning

confidence: 99%

Mimic Spaceflight: Microgravity Induces Bone Injury

Zhang¹

2023

HSET

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Astronauts take the risk of health problems during spaceflight. One of the most severe problems is bone injury. Though astronauts experience exercises before spaceflight, bone injury is still induced by anti-gravity. To try best to mimic the condition free from the force of gravity, most studies have explored the mechanism of bone injury under microgravity condition. In microgravity environment, osteocytes, osteoclasts, osteoblasts, and mesenchymal stem cells are all identified to alter, contributing to bone loss and function defects of mineralization. Ulteriorly, three typical molecular regulatory pathways including NF-κB pathway, RhoA/integrin pathway, and calcium pathway have been discovered till now, which reveals the possible molecular mechanism. In this article, the specific alterations of cells associated with bone and the three pathways mentioned above will be presented. Different systems are found to related to bone alterations modulated by microgravity as well. So far, the mechanism has not been completely comprehended because of its complexity. However, the current discoveries about mechanism are still valuable. According to regulatory mechanism, new therapies are designed to improve bone injury aiming to help astronauts.

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Microgravity Effects and Aging Physiology: Similar Changes or Common Mechanisms?

Ratushnyy,

Buravkova

2023

Biochemistry Moscow

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Secretory Activity of Mesenchymal Stromal Cells with Different Degree of Commitment under Conditions of Simulated Microgravity

Cited by 4 publications

References 13 publications

Specifics of Cryopreservation of Hydrogel Biopolymer Scaffolds with Encapsulated Mesenchymal Stem Cells

Specifics of Cryopreservation of Hydrogel Biopolymer Scaffolds with Encapsulated Mesenchymal Stem Cells

Mimic Spaceflight: Microgravity Induces Bone Injury

Microgravity Effects and Aging Physiology: Similar Changes or Common Mechanisms?

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