Secretoneurin is a secretogranin-2 derived hormonal peptide in vertebrate neuroendocrine systems

Mitchell, Kimberly; Mikwar, Myy; Fonte, Dillon Da; Lu, Chunyu; Tao, Binbin; Peng, Di; Erandani, W.K.C. Udeesha; Hu, Wei; Trudeau, Vance L.

doi:10.1016/j.ygcen.2020.113588

Cited by 16 publications

(9 citation statements)

References 101 publications

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“…Meanwhile, in electric fish, SN and AVP were also confirmed to modulate social behavior [50]. There is also evidence that these hypothalamic neuropeptides (SN, OXT, and AVP) regulate social behaviors by regulating the HPG axis [53,54]. Consequently, TEB might influence the social behavior of zebrafish by interfering with the expression of genes involved in social behavior and the HPG axis, eventually affecting the reproduction of zebrafish.…”

Section: Discussionmentioning

confidence: 97%

“…Additionally, isotocin (OXT) and vasotocin (AVP) which are co-expressed with SCG2 are well-known regulators of social behavior [50,52]. A growing body of evidence supports that SCG2, OXT, and AVP are associated with social behavior [53,54]. Hence, for the sake of exploring the mechanisms of TEB affecting social behavior, we further examined the transcriptions of behavior-related genes (scg2a, scg2b, oxt, and avp).…”

Section: Discussionmentioning

confidence: 99%

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Molecular Mechanisms of Tebuconazole Affecting the Social Behavior and Reproduction of Zebrafish

Yan

Lü

et al. 2023

IJERPH

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The aim of this study was to explore the underlying mechanism of adverse effects caused by tebuconazole (TEB) on the reproduction of aquatic organisms In the present study, in order to explore the effects of TEB on reproduction, four-month-old zebrafish were exposed to TEB (0, DMSO, 0.4 mg/L, 0.8 mg/L, and 1.6 mg/L) for 21 days. After exposure, the accumulations of TEB in gonads were observed and the cumulative egg production was evidently decreased. The decline of fertilization rate in F1 embryos was also observed. Then the changes in sperm motility and histomorphology of gonads were discovered, evaluating that TEB had adverse effects on gonadal development. Additionally, we also found the alternations of social behavior, 17β-estradiol (E2) level, and testosterone (T) level. Furthermore, the expression levels of genes involved in the hypothalamic-pituitary-gonadal (HPG) axis and social behavior were remarkably altered. Taken together, it could be concluded that TEB affected the egg production and fertilization rate by interfering with gonadal development, sex hormone secretion, and social behavior, which were eventually attributed to the disruption of the expressions of genes associated with the HPG axis and social behavior. This study provides a new perspective to understanding the mechanism of TEB-induced reproductive toxicity.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Molecular Mechanisms of Tebuconazole Affecting the Social Behavior and Reproduction of Zebrafish

Yan

Lü

et al. 2023

IJERPH

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“…To conclude, SgII is conserved in different species (Figure S10, Supporting Information), and LDCVs are critical for neuropeptide and hormone release in many endocrine and neuroendocrine cells as well as neurons to regulate many important physiological processes. [ 87–90 ] In the present work, we provide sufficient experiments to prove the causal relationship between protein liquid–liquid phase separation and the regulation of LDCV size and neurotransmitters quantal release. Protein phase separation is considered to be a new feature for some proteins and it plays a vital role in many physiological processes.…”

Section: Discussionmentioning

confidence: 87%

Tuning the Size of Large Dense‐Core Vesicles and Quantal Neurotransmitter Release via Secretogranin II Liquid–Liquid Phase Separation

Lin

Hang

et al. 2022

Advanced Science

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Large dense‐core vesicles (LDCVs) are larger in volume than synaptic vesicles, and are filled with multiple neuropeptides, hormones, and neurotransmitters that participate in various physiological processes. However, little is known about the mechanism determining the size of LDCVs. Here, it is reported that secretogranin II (SgII), a vesicle matrix protein, contributes to LDCV size regulation through its liquid–liquid phase separation in neuroendocrine cells. First, SgII undergoes pH‐dependent polymerization and the polymerized SgII forms phase droplets with Ca2+ in vitro and in vivo. Further, the Ca2+‐induced SgII droplets recruit reconstituted bio‐lipids, mimicking the LDCVs biogenesis. In addition, SgII knockdown leads to significant decrease of the quantal neurotransmitter release by affecting LDCV size, which is differently rescued by SgII truncations with different degrees of phase separation. In conclusion, it is shown that SgII is a unique intravesicular matrix protein undergoing liquid–liquid phase separation, and present novel insights into how SgII determines LDCV size and the quantal neurotransmitter release.

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“…Both knockouts exhibit lower spawning rates and reduced courtship behavior. Although secretogranin is generally considered to have a positive effect on the reproductive axis, mainly by increasing the secretion of LH when administered synthetically [67, 68], it has been seen to have low expression in the pituitary of the sexually mature orange-spotted grouper [69] and goldfish [70]. Low levels of scg2b in the pituitary at the mature phase of the gonad have been attributed to the negative regulation of T on scg2b expression [70].…”

Section: Discussionmentioning

confidence: 99%

Identifying the Interaction of the Brain and the Pituitary in Social – and Reproductive – State of Tilapia by Transcriptome Analyses

Hollander-Cohen¹,

Meir²,

Shulman³

et al. 2022

Neuroendocrinology

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Introduction: As in all vertebrates, reproduction in fish is regulated by GnRH control on gonadotrophic hormones (GtH) activity. However, the neuroendocrine factors that promote GnRH and GtH activity are unknown. In Nile tilapia (Oreochromis niloticus), sexual activity and reproduction ability depend on social rank; only dominant males and females reproduce. Here, this characteristic of dominant fish allows us to compare brain and pituitary gene expression in animals that do and do not reproduce, aiming to reveal mechanisms that regulate reproduction. Methods: an extensive transcriptome analysis was performed, combining two sets of transcriptomes: a novel whole-brain and pituitary transcriptome of established dominant and subordinate males, together with a cell-specific transcriptome of LH and FSH cells. Pituitary incubation assay validated the direct effect of steroid application on chosen genes and GtH secretion. Results: in most dominant fish, as determined behaviorally, the gonadosomatic index was higher than in subordinate fish, and the leading upregulated pituitary genes were those coding for GtHs. In the brain, various neuropeptide genes, including isotocin, cholecystokinin, and MCH, were upregulated; these may be related to reproductive status through effects on behavior and feeding. In a STRING network analysis combining the two transcriptome sets, brain aromatase, highly expressed in LH cells, is the most central gene with the highest number of connections. In the pituitary incubation assay, testosterone and estradiol increased the secretion of LH and specific gene transcription. Conclusions: the close correlation between behavioral dominance and reproductive capacity in tilapia allows unraveling novel genes that may regulate the HPG axis, highlighting aromatase as the main factor affecting the brain and pituitary in maintaining a sexually active organism.

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Secretoneurin is a secretogranin-2 derived hormonal peptide in vertebrate neuroendocrine systems

Cited by 16 publications

References 101 publications

Molecular Mechanisms of Tebuconazole Affecting the Social Behavior and Reproduction of Zebrafish

Molecular Mechanisms of Tebuconazole Affecting the Social Behavior and Reproduction of Zebrafish

Tuning the Size of Large Dense‐Core Vesicles and Quantal Neurotransmitter Release via Secretogranin II Liquid–Liquid Phase Separation

Identifying the Interaction of the Brain and the Pituitary in Social – and Reproductive – State of Tilapia by Transcriptome Analyses

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