Secretome of Adipose-Derived Stem Cells Cultured in Platelet Lysate Improves Migration and Viability of Keratinocytes

Abstract

Chronic wounds depict a silent epidemic challenging medical professionals worldwide. Regenerative medicine uses adipose-derived stem cells (ADSC) in promising new therapies. In this study, platelet lysate (PL) as a xenogen-free substitute for foetal bovine serum (FBS) in ADSC culture was used to create an ADSC secretome containing cytokines for optimal wound healing conditions. The ADSC secretome was tested on keratinocytes for migrational behaviour and viability. Therefore, human ADSC were characterized under FBS (10%) and PL (5% and 10%) substitution, regarding morphology, differentiation, viability, gene and protein expression. ADSC were then cultured in 5% PL and their secretome was used for stimulation of keratinocyte migration and viability. To enhance the effect, ADSC were treated with Epithelial Growth Factor (EGF, 100 ng/mL) and hypoxia (1% O₂). In both PL and FBS groups, ADSC expressed typical stem cell markers. PL induced a significantly higher increase in cell viability compared to FBS substitution. ADSC secretome contained various beneficial proteins which enhance the wound healing capacity of keratinocytes. This could be optimized treating ADSC with hypoxia and EGF. In conclusion, the study shows that ADSC cultivated in 5 % PL can effectively support wound healing conditions and can be considered as a promising new therapy for individual treatment of chronic wound disorders.

Adipose-derived stem cells in diabetic foot care: Bridging clinical trials and practical application

“…Studies have explored various methods to augment the healing capabilities of ADSCs. For instance, Hermann et al [ 56 ] found that incorporating platelet lysate into ADSC culture improved keratinocyte migration and viability. Carolina Oses et al [ 57 ] demonstrated that preconditioning ADSCs with Deferoxamine, a hypoxic mimetic, increased the secretion of neuroprotective and angiogenic factors.…”

Recombinant human fibroblast growth factor 7 obtained from stable Chinese hamster ovary cells enhances wound healing

“…[6,7] During the granulation process of tissue development, various immune cells, including macrophages, mast cells, and T lymphocytes, secrete FGFs, leading to an increased FGF concentration at the wound site. [8][9][10] In turn, this promotes the growth of fibroblasts, epithelial cells, and keratinocytes, facilitating angiogenesis, epithelialization, and collagen formation, all of which contributes to promoting the wound healing process. [11][12][13][14] FGF7 is widely regarded as a significant growth factor for wound healing.…”

Application of hypoxia-mesenchymal stem cells in treatment of anaerobic bacterial wound infection: wound healing and infection recovery

“…MSCs have the potential to transform into skin substitutes, thereby acting as a viable alternative to dermal fibroblasts in the process of epidermis generation and skin wound healing. They are capable of differentiating into several cell types in injured areas, including dermal fibroblasts, keratinocytes, and endothelial cells ( Hermann et al, 2023 ; Mahmoudvand et al, 2023 ; Nilforoushzadeh et al, 2023 ). Apart from their differential ability, MSCs have additional characteristics, such as being easily harvested and showing minimal immunogenicity.…”