Secretome Analysis: Reading Cellular Sign Language to Understand Intercellular Communication

Wu, Wei; Krijgsveld, Jeroen

doi:10.1016/j.mcpro.2023.100692

Cited by 2 publications

(2 citation statements)

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“…Once activated, TLR transduction signals lead to an increase of signaling cascades, causing expression of antimicrobial agents and the pro-inflammatory cytokines and chemokines for neutrophil recruitment. The importance of the expression of these extracellular bioactive molecules in pathogenicity of a disease has been well discussed in a recent review on secretome analysis . Interestingly, 25 of the 64 verified biomarker proteins in this study overlapped with known secretome proteins listed in The Human Protein Atlas .…”

Section: Resultsmentioning

confidence: 59%

“…The importance of the expression of these extracellular bioactive molecules in pathogenicity of a disease has been well discussed in a recent review on secretome analysis. 42 Interestingly, 25 of the 64 verified biomarker proteins in this study overlapped with known secretome proteins listed in The Human Protein Atlas. 31 Also, many of the other biomarkers found have similar names or functions as others in the Protein Atlas secretome list.…”

Section: Perturbations At the Ocular Surface In Ss-dedmentioning

confidence: 72%

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Discovery and Verification of Sjögren’s Syndrome Protein Biomarkers in Tears by Targeted LC-MRM

Lépine,

Robert,

Sleno

2024

J. Proteome Res.

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Sjogren's syndrome (SS) is an autoimmune rheumatic disorder characterized by exocrine gland dysfunction, mainly from the lacrimal and salivary glands. The disease causes severe aqueous dry eye syndrome (DED) and is associated with high rates of complications, including corneal ulceration, scaring, and perforation. Systemic complications may occur as well as a higher risk of developing lymphoma. Diagnosis of SS-DED is often delayed and difficult to establish. With the aim of discovering biomarkers to help discriminate SS-DED patients, a combination of untargeted and targeted LC-MS/MS analyses were performed on tear samples collected on Schirmer strips and subjected to tryptic digestion. Following the analysis of three cohorts and the development of two targeted LC-sMRM methods for the verification of putative biomarkers found in the first cohort of samples, 64 proteins could be linked to Sjogren's syndrome, in the hopes of helping to confirm diagnoses as well as potentially stratifying the severity of disease in these patients. Proteins that were increased in SS-DED showed activation of the immune system and alterations in homeostasis. Several proteases and protease inhibitors were found to be significantly changing in SS-DED, as well as a consistent decrease in specific proteins known to be secreted by the lacrimal gland.

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Section: Resultsmentioning

confidence: 59%

Section: Perturbations At the Ocular Surface In Ss-dedmentioning

confidence: 72%