Secretome analysis of atherosclerotic and non-atherosclerotic arteries reveals dynamic extracellular remodeling during pathogenesis

Modification of the Secretion Pattern of Proteases, Inflammatory Mediators, and Extracellular Matrix Proteins by Human Aortic Valve is Key in Severe Aortic Stenosis

“…Importantly, particular proteins identified are involved in the atherosclerotic process, supporting the emerging hypothesis of a common link between both pathologies. On the other hand, gelsolin has here been demonstrated to be a high sensitivity biomarker of AS, with an opposite trend of secretion to plasma than atherosclerotic arterial tissue (13), which makes it a potential ideal discriminant biomarker of both pathologies. Our approach also exemplifies the development of specific and sensitive assays to validate biomarkers in the secretome.…”

“…Among all proteins tested in secretome samples, gelsolin showed the same trend (increased in AS group) independently of the strategy used for validation and in all the approaches tested (WB and SRM). Interestingly, this protein followed the opposite trend in the secretome from atherosclerotic coronary arteries (13). For all these reasons and considering the limited knowledge about common and specific operating mechanisms in AS and atherosclerosis pathologies, we further validate gelsolin response in a new, wider and independent cohort of samples.…”

“…As such, the aortic valve secretome has emerged as an attractive target to further understand the AS pathogenic process. Tissue secretomes provide a more accurate model of the in vivo situation and, by minimizing serum contaminants, they facilitate the detection of low abundance proteins secreted into the blood (13).…”

Hypothesis-free secretome analysis of thoracic aortic aneurysm reinforces the central role of TGF-β cascade in patients with bicuspid aortic valve

“…Aortic tissues were cultured according to de la Cuesta et al with modifications [10]. Specimens (n = 8) from surgery were dissected in order to peel off the tunica adventitia.…”

Contribution of proteomics to the management of vascular disorders

“…Through this approach, proteins released into the blood from the vascular tissue can be discovered, constituting a potentially reliable source of disease biomarkers. In this sense, a characteristic secretome of atherosclerotic arteries [48], aortic valves with stenosis [49] and aortic intraluminal thrombi [50] has been reported, that is potentially relevant in the diagnosis of these pathologies and/or in the definition of novel therapeutic targets.…”