Secretion systems play a critical role in resistance to predation by Bdellovibrio bacteriovorus

Aharon, Einav; Mookherjee, Abhirup; Pérez‐Montaño, Francisco; Sathyamoorthy, Rajesh; Burdman, Saul; Jurkevitch, Édouard

doi:10.1016/j.resmic.2021.103878

Cited by 10 publications

(12 citation statements)

References 51 publications

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“…In addition, we found that type II and type IV secretion systems were highly expressed in several MAGs (i.e. Acidobacteria, Comamonadaceae ) which might have been involved in predation, defense, and conjugation activities between microorganisms ( Aharon et al., 2021 ; Sgro et al., 2019 ). However, some of these MAGs might also degrade APAP transformation products via their highly-expressed dioxygenases (i.e.…”

Section: Resultsmentioning

confidence: 96%

Microbial paracetamol degradation involves a high diversity of novel amidase enzyme candidates

Rios‐Miguel¹,

Smith²,

Cremers³

et al. 2022

Water Research X

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Section: Resultsmentioning

confidence: 96%

Microbial paracetamol degradation involves a high diversity of novel amidase enzyme candidates

Rios‐Miguel¹,

Smith²,

Cremers³

et al. 2022

Water Research X

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“…Yet, prey transport systems seem to be directly implicated in the ability of the predator B . bacteriovorus to exploit a particular prey (Aharon et al ., 2021). Whether changes in the display or expression of these pathways are linked to phenotypic, plastic resistance remains to be tested.…”

Section: Resultsmentioning

confidence: 99%

“…Genes involved in resistance/sensitivity to BALO predation appear to differ between prey species (Duncan et al, 2018), and even between prey strains of the same species (Aharon et al, 2021). Yet, prey transport systems seem to be directly implicated in the ability of the predator B. bacteriovorus to exploit a particular prey (Aharon et al, 2021). Whether changes in the display or expression of these pathways are linked to phenotypic, plastic resistance remains to be tested.…”

Section: Plastic Phenotypic Resistance To Predation and Antibiotic Pe...mentioning

confidence: 99%

“…Furthermore, genetic resistance in BALOs is little understood: BALOs strains belonging to the same species differ in prey range, and different prey strains belonging to the same species may also be differentially preyed upon by a particular BALO (Dashiff et al, 2011). Genes involved in resistance/sensitivity to BALO predation appear to differ between prey species (Duncan et al, 2018), and even between prey strains of the same species (Aharon et al, 2021). Yet, prey transport systems seem to be directly implicated in the ability of the predator B. bacteriovorus to exploit a particular prey (Aharon et al, 2021).…”

Section: Plastic Phenotypic Resistance To Predation and Antibiotic Pe...mentioning

confidence: 99%

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Predation of antibiotic persister bacteria by the predatory bacteriumBdellovibrio bacteriovorus

Matan

Jurkevitch

2022

Environ Microbiol Rep

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Antibiotic resistance (AR) in bacteria is an urgent and global health issue, encompassing clinical, agricultural, terrestrial and aquatic environments. AR is not only expressed through genetic resistance. It is also found in bacteria in a small fraction of populations exhibiting antibiotic 'persister' states, thereby acting as a reservoir for re-growth. The predatory bacteria Bdellovibrio and like organisms (BALOs) can consume AR pathogens, reducing populations by orders of magnitude. However, it is not known if antibiotic persistence shields cells from attack by BALOs. Moreover, BALOs do not eradicate prey populations; rather, a small fraction of the population exhibits plastic phenotypic predation resistance. In this study, we show that Escherichia coli antibiotic persisters obtained by exposure of a sensitive population to ampicillin are preyed upon by Bdellovibrio bacteriovorus as much as control populations. Furthermore, plastic phenotypic predation-resistant E. coli populations do not show increased AR. In conclusion, antibiotic persistence and plastic phenotypic predation resistance do not directly affect one another. This knowledge may be important in the use of BALOs to prevent the spread of AR.

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“…Acidobacteria, Comamonadaceae) which might have been involved in predation, defense, and conjugation activities between microorganisms (Aharon et al, 2021;Sgro et al, 2019). However, some of these MAGs might also degrade APAP transformation products via their highly-expressed dioxygenases (i.e.…”

mentioning

confidence: 99%

Microbial paracetamol degradation involves a high diversity of novel amidase enzyme candidates

Rios‐Miguel

Smith

Cremers

et al. 2022

Preprint

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Pharmaceuticals are relatively new to nature and often not completely removed in wastewater treatment plants (WWTPs). Consequently, these micropollutants end up in water bodies all around the world posing a great environmental risk. One exception to this recalcitrant conversion is paracetamol, whose full degradation has been linked to several microorganisms. However, the genes and corresponding proteins involved in microbial paracetamol degradation are still elusive. In order to improve our knowledge of the microbial paracetamol degradation pathway, we inoculated a bioreactor with sludge of a hospital WWTP (Pharmafilter, Delft, NL) and fed it with paracetamol as the sole carbon source. Paracetamol was fully degraded without any lag phase and the enriched microbial community was investigated by metagenomic and metatranscriptomic analyses, which demonstrated that the microbial community was very diverse. Dilution and plating on paracetamol-amended agar plates yielded two Pseudomonas sp. isolates: a fast-growing Pseudomonas sp. that degraded 200 mg/L of paracetamol in approximately 10 hours while excreting a dark brown component to the medium, and a slow-growing Pseudomonas sp. that degraded paracetamol without obvious intermediates in more than 90 days. Each Pseudomonas sp. contained a different highly-expressed amidase (31% identity to each other). These amidase genes were not detected in the bioreactor metagenome suggesting that other as-yet uncharacterized amidases may be responsible for the first biodegradation step of paracetamol. Uncharacterized deaminase genes and genes encoding dioxygenase enzymes involved in the catabolism of aromatic compounds and amino acids were the most likely candidates responsible for the degradation of paracetamol intermediates based on their high expression levels in the bioreactor metagenome and the Pseudomonas spp. genomes. Furthermore, cross-feeding between different community members might have occurred to efficiently degrade paracetamol and its intermediates in the bioreactor. This study increases our knowledge about the ongoing microbial evolution towards biodegradation of pharmaceuticals and points to a large diversity of (amidase) enzymes that are likely involved in paracetamol metabolism in WWTPs.

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Secretion systems play a critical role in resistance to predation by Bdellovibrio bacteriovorus

Cited by 10 publications

References 51 publications

Microbial paracetamol degradation involves a high diversity of novel amidase enzyme candidates

Microbial paracetamol degradation involves a high diversity of novel amidase enzyme candidates

Predation of antibiotic persister bacteria by the predatory bacteriumBdellovibrio bacteriovorus

Microbial paracetamol degradation involves a high diversity of novel amidase enzyme candidates

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