Secretion of Type IV Collagen by Keratinocytes of Human Adult

Oguchi, Motoi; Kobayasi, T; Asboe-Hansen, G

doi:10.1111/1523-1747.ep12275360

Cited by 34 publications

(18 citation statements)

References 12 publications

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“…6E & 6F), suggesting the cultured keratinocytes maintain their appropriate phenotype for synthesis and deposition of both molecules [37, 38]. It was noted that these molecules distributed across the entire epidermal domain instead of limited to the epidermal-dermal junction.…”

Section: Discussionmentioning

confidence: 99%

Rapid creation of skin substitutes from human skin cells and biomimetic nanofibers for acute full-thickness wound repair

Mahjour

Yang

et al. 2015

Burns

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Creation of functional skin substitutes within a clinically acceptable time window is essential for timely repair and management of large wounds such as extensive burns. The aim of this study was to investigate the possibility of fabricating skin substitutes via a bottom-up nanofiber-enabled cell assembly approach and using such substitutes for full-thickness wound repair in nude mice. Following a layer-by-layer (L-b-L) manner, human primary skin cells (fibroblasts and keratinocytes) were rapidly assembled together with electrospun polycaprolactone (PCL)/collagen (3:1 w/w, 8% w/v) nanofibers into 3D constructs, in which fibroblasts and keratinocytes were located in the bottom and upper portion respectively. Following culture, the constructs developed into a skin-like structure with expression of basal keratinocyte markers and deposition of new matrix while exhibiting good mechanical strength (as high as 4.0 MPa by 14 days). Treatment of the full-thickness wounds created on the back of nude mice with various grafts (acellular nanofiber meshes, dermal substitutes, skin substitutes and autografts) revealed that 14-day-cultured skin substitutes facilitated a rapid wound closure with complete epithelialization comparable to autografts. Taken together, skin-like substitutes can be formed by L-b-L assembling human skin cells and biomimetic nanofibers and they are effective to heal acute full-thickness wounds in nude mice.

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Section: Discussionmentioning

confidence: 99%

Rapid creation of skin substitutes from human skin cells and biomimetic nanofibers for acute full-thickness wound repair

Mahjour

Yang

et al. 2015

Burns

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“…However, a nonscarring (inflammatory) phase is seen in EBA, and only 1 case of nonscarring EBA has been reported [12]. Then, a few cases of EBA with esophageal involvement were described [13, 14, 15, 16].…”

Section: Discussionmentioning

confidence: 99%

A Case of an Inflammatory Variant of Epidermolysis bullosa acquisita: Chronic Bullous Dermatosis Associated with Nonscarring Mucosal Blisters and Circulating IgG Anti-Type-VII-Collagen Antibody

et al. 1998

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A 42-year-old man showed prominent blistering lesions of the mouth and esophagus in addition to a few bullous lesions of the skin. Direct immunofluorescence microscopy revealed distinct linear deposition of IgG and C3 at the epidermal basement membrane zone where slight deposition of IgA and IgM was also observed. In direct immunoelectron-microscopic examination, antibody was detected in the sublamina densa of the basement membrane zone. Immunoblot analysis with dermal extracts demonstrated that the patient’s serum contained circulating IgG antibodies against the 290-kD protein, which comigrated with type VII collagen. The lesions healed without any scars. The results of these studies corresponded to the laboratory findings in epidermolysis bullosa acquisita (EBA), although the clinical features were distinct from classic EBA.

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“…In our experience, we also obtained very effective healing of chronic skin ulcers with cultured epidermal allografts [4], The wounds healed by cell migration from the wound edge. It is also known that keratinocytes secrete a variety of cytokines including trans forming growth factors [12J, fibronectin [13], interleukin [14], keratinocyte-derived growth factor [15] and type IV collagen [16]. So the healing of our patient's wounds might be ascribed to the action of unknown cytokines or basement components elaborated by the cultured epidermal allo grafts.…”

Section: Discussionmentioning

confidence: 99%

Treatment of Epidermolysis bullosa with Human Cultured Epidermal Allografts

Roseeuw

Raeve²,

Dangoisse³

et al. 1994

Dermatology

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Junctional epidermolysis bullosa letalis type Herlitz Pearson is a genetically determined, life-threatening disease. Effective therapy has been lacking to date. Therefore any therapy that improves wound healing would be beneficial for these patients. Cultured epidermal grafts are known to enhance wound epithelialization and have been used with success in some epidermolysis bullosa disorders. Encouraged by these reports, we grafted cultured allogeneic keratinocytes to an infant with a junctional epidermolysis bullosa letalis type.

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Secretion of Type IV Collagen by Keratinocytes of Human Adult

Cited by 34 publications

References 12 publications

Rapid creation of skin substitutes from human skin cells and biomimetic nanofibers for acute full-thickness wound repair

Rapid creation of skin substitutes from human skin cells and biomimetic nanofibers for acute full-thickness wound repair

A Case of an Inflammatory Variant of Epidermolysis bullosa acquisita: Chronic Bullous Dermatosis Associated with Nonscarring Mucosal Blisters and Circulating IgG Anti-Type-VII-Collagen Antibody

Treatment of Epidermolysis bullosa with Human Cultured Epidermal Allografts

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