Secretion of type E prostaglandins (PGE) by Syrian hamster tumor cells during contact in vitro with natural killer cells

Klyuchareva, T. E.; Матвеева, В. А.; Bassalyk, A. S.; Kushlinskii, N. E.

doi:10.1007/bf00835705

Cited by 4 publications

(8 citation statements)

References 4 publications

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“…The expression of the last two properties was particularly interesting, since these two discrete and biochemically unrelated cell properties might indeed be expected to be selected in vivo independently of each other. The relation of PGE-releasing activity to spontaneous MA was also interesting to evaluate in a large number of selected STHE cell lines, since this was first observed in cells of one highly metastatic variant of the STHE strain (Kluchareva et al, 1988).…”

Section: Resultsmentioning

confidence: 99%

“…0 ml) and grown on the bottom of 3 (for each sample) scintillation flasks for 20 hr at 37°C. Incorporation of 3H-TdR was determined in nuclei of control cells (considered as 100%) and of cells treated with different doses of PGE-releasing activity of each subline was determined by a biological method based on the original data of Kluchareva et al (1988). These show that close contact between NK cells and highly metastatic variants of STHE cells (but not parental STHE cells) for 20 min rapidly leads to release of PGE into the mixed-cell culture medium.…”

Section: Methodsmentioning

confidence: 99%

“…Calculations have shown that H,Of cells occur as a very rare event, probably in much less than 0.1% of the parental cell population (Deichman and Vendrov, 1986). At least one in vivo selected H,OF, highly-metastatic STHE cell variant (designated STHE-LM-8), in contrast to the parental cells, was able to release very rapidly (within a few minutes) large amounts of prostaglandin E (PGE) (Kluchareva et al, 1988). PGEs effectively inhibit NK cell cytotoxicity (Droller et al, 1979;Brunda et a!., 1980).…”

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confidence: 99%

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Clustering of discrete cell properties essential for tumorigenicity and metastasis. I. Studies of syrian hamster embryo fibroblasts spontaneously transformed in vitro

Deichman

Kluchareva

Матвеева

et al. 1989

Intl Journal of Cancer

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The expression of two discrete cell properties related to the host natural effector mechanisms, i.e., resistance to damage by H2O2, a cytotoxic product of activated macrophages, and the ability to secrete PGE, which inhibits NK-cell cytotoxicity, has been examined in parental Syrian hamster embryo cells spontaneously transformed in vitro (STHE strain) and in 18 in vivo selected sublines. In all cell variants, resistance to H2O2 and PGE-releasing activity were either both expressed, or not expressed at all. Parental STHE cells and 5 variants selected in vivo, which were equally highly susceptible to H2O2-induced damage, did not release any detectable amount of PGE upon contact with NK cells. In contrast, 13 other STHE variants selected in vivo and characterized by their resistance to H2O2, all released PGE upon contact with NK cells. Thus, these two biochemically unrelated cell phenotypic characteristics are likely to be either simultaneously selected in vivo, or united in cluster which pre-exist or appear in rare cell variants of the parental cell population in the conditions of in vivo natural selection pressure.

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

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Clustering of discrete cell properties essential for tumorigenicity and metastasis. I. Studies of syrian hamster embryo fibroblasts spontaneously transformed in vitro

Deichman

Kluchareva

Матвеева

et al. 1989

Intl Journal of Cancer

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“…On the one hand, experiments with cultured animal tissues showed that the contact of effector cells with tumor ceils stimulates the release of PGE from the surface of tumor cells and lowers the cytotoxic activity of immunocompetent cells [3,4]. This indicates that PGE plays an important role in the mechanisms whereby tumor is protected against the cytotoxic effect of immunocompetent cells [9].…”

Section: Resultsmentioning

confidence: 98%

“…Immunocompetent cells are involved in the processes of tumor metastasizing [3,9]. PGE is known to inhibit the cytostatic activity of effector cells (natural killer cells, macrophages, and neutrophils), thus promoting metastasizing [4].…”

Section: Resultsmentioning

confidence: 99%

Neoadjuvant chemotherapy and the cyclooxygenase pathway of arachidonic acid metabolism in osteogenic sarcoma

Kushlinskiĭ

IuN

et al. 1996

Bull Exp Biol Med

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Arachidonic acid metabolites are measured in primary osteogenic sarcomas, benign neoplasms, and tumor-like bone lesions. In typical osteogenic sarcomas, the contents of E and F2~ prostaglandins are significantly higher than in benign bone tumors or tumor-like bone lesions. Neoadjuvant chemotherapy has different effects on the levels of prostaglandins E and F2,, thromboxane B v and prostaglandin F~ in osteogenic sarcomas, its effectiveness depending on the mechanism of drug action and individual sensitivity of tumor cells to this drug. A negative correlation is established between the content of prostaglandins E in osteogenic sarcoma and the degree of therapeutic pathomorphosis of the tumor in patients treated with adriamycin during the preoperative period. A single transfusion of a suspension of nonactivated allogenic bone marrow ceils markedly reduces the content of prostaglandins E in osteogenic sarcoma.

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Clustering of discrete cell properties essential for tumorigenicity and metastasis. II. Studies of syrian hamster embryo fibroblasts transformed by rous sarcoma virus

Deichman

Kashleva

Kluchareva

et al. 1989

Intl Journal of Cancer

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Tumorigenic and metastasizing activities (TGA; MA) and susceptibility, or resistance to H2O2 and PGE-releasing activity (H2O2R + PGEs+ phenotype) have been examined in 6 Syrian hamster embryo cell strains transformed in vitro with Rous sarcoma viruses (Schmidt-Ruppin and Prague strains). Early observations of extremely high level of TGA and even MA of RSV-SR-transformants never selected in vivo have been confirmed. The correspondence of these properties with a high level of expression of H2O2R + PGEs+ phenotype and its clustering character were demonstrated in 4 RSV-SR transformants, while significantly lower expression of all these characteristics, including TGA, was observed in 2 RSV-Prague transformants. High level of spontaneous MA was noticed in some RSV-SR transformants. A tumor cell line induced in vivo by RSV-SR did not differ from the cell strain transformed in vitro by RSV-SR. Inhibition of H2O2R + PGEs+ phenotype in one of RSV-SR transformants was obtained with non-toxic doses of BCNU and indomethacin, leading to a marked decrease of TGA.

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Secretion of type E prostaglandins (PGE) by Syrian hamster tumor cells during contact in vitro with natural killer cells

Cited by 4 publications

References 4 publications

Clustering of discrete cell properties essential for tumorigenicity and metastasis. I. Studies of syrian hamster embryo fibroblasts spontaneously transformed in vitro

Clustering of discrete cell properties essential for tumorigenicity and metastasis. I. Studies of syrian hamster embryo fibroblasts spontaneously transformed in vitro

Neoadjuvant chemotherapy and the cyclooxygenase pathway of arachidonic acid metabolism in osteogenic sarcoma

Clustering of discrete cell properties essential for tumorigenicity and metastasis. II. Studies of syrian hamster embryo fibroblasts transformed by rous sarcoma virus

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