Secretion of Thioredoxin Peroxidase Protein of Cat Liver Fluke Opisthorchis felineus during Modeling of Experimental Opisthorchiasis

Petrenko, Valery A.; Pakharukova, Maria Y.; Ковнер, А. В.; Lvova, Maria; Lyakhovich, V. V.; Mordvinov, Viatcheslav A.

doi:10.1007/s10517-017-3710-5

Cited by 9 publications

(6 citation statements)

References 11 publications

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“…thioredoxin and TPx, can prevent apoptosis (Sripa et al ., 2012 a ). Opisthorchis viverrini - and O. felineus -derived substances are internalized by the bile duct epithelium (Chaiyadet et al ., 2015; Petrenko et al ., 2017; Pakharukova et al ., 2019 c ). Cholangiocytes in culture can capture exosomes of O. viverrini , which is followed by elevated cell proliferation, IL-6 expression and pronounced alterations of the cell phenotype (Chaiyadet et al ., 2015).…”

Section: Resultsmentioning

confidence: 99%

Similarities and differences among the Opisthorchiidae liver flukes: insights from Opisthorchis felineus

Pakharukova

Mordvinov

2022

Parasitology

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The foodborne liver trematode Opisthorchis felineus (Rivolta, 1884) is a member of the triad of phylogenetically related epidemiologically important Opisthorchiidae trematodes, which also includes O. viverrini (Poirier, 1886) and Clonorchis sinensis (Loos, 1907). Despite similarity in the life cycle, Opisthorchiidae liver flukes also have marked differences. Two species (O. viverrini and C. sinensis) are recognized as Group 1A biological carcinogens, whereas O. felineus belongs to Group 3A. In this review, we focus on these questions: Are there actual differences in carcinogenicity among these 3 liver fluke species? Is there an explanation for these differences? We provide a recent update of our knowledge on the liver fluke O. felineus and highlight its differences from O. viverrini and C. sinensis. In particular, we concentrate on differences in the climate of endemic areas, characteristics of the life cycle, the range of intermediate hosts, genomic and transcriptomic features of the pathogens, and clinical symptoms and morbidity of the infections in humans. The discussion of these questions can stimulate new developments in comparative studies on the pathogenicity of liver flukes and should help to identify species-specific features of opisthorchiasis and clonorchiasis pathogenesis.

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Section: Resultsmentioning

confidence: 99%

Similarities and differences among the Opisthorchiidae liver flukes: insights from Opisthorchis felineus

Pakharukova

Mordvinov

2022

Parasitology

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“…5 ). Proposed mechanisms behind the carcinogenicity of liver fluke infection include biliary-epithelium damage by parasites, long-lasting immune-system–mediated pathogenesis, and effects of parasite-derived substances that are internalized by the bile duct epithelium ( Pakharukova et al, 2019b ; Petrenko et al, 2017 ; Smout et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Global changes in gene expression related to Opisthorchis felineus liver fluke infection reveal temporal heterogeneity of a mammalian host response

Pakharukova

Zaparina

Baginskaya

et al. 2022

Food and Waterborne Parasitology

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“…Some O . felineus products are highly immunogenic [11], they can interact with the epithelium and accumulate inside the cells [10]. It should be also noted that liver fluke-induced CCA is a multifactorial etiology and other factors, such as Helicobacter infection within the biliary tract or presence of carcinogens and their precursors in food might enhance CCA development [26].…”

Section: Discussionmentioning

confidence: 99%

“…Immunoblotting was performed as described elsewhere [11]. Antibodies and dilutions employed in this study include anti–TNF-α (1:2000; cat.…”

Section: Methodsmentioning

confidence: 99%

Opisthorchis felineus infection provokes time-dependent accumulation of oxidative hepatobiliary lesions in the injured hamster liver

et al. 2019

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Opisthorchiasis caused by food-borne trematode Opisthorchis felineus is a substantial public health problem, with 17 million persons infected worldwide. This chronic disease is associated with hepatobiliary inflammation, cholangiocyte dysplasia, cholangiofibrosis, intraepithelial neoplasia, and even cholangiocarcinoma among chronically infected individuals. To provide first insights into the mechanism by which O . felineus infection causes precancerous liver lesions, we investigated the level of oxidative stress (lipid peroxidation byproducts and 8-hydroxy-2′-deoxyguanosine) as well as the time course profiles of chronic inflammation and fibrogenesis markers in the dynamics of opisthorchiasis from 1 month to 1.5 years postinfection in an experimental model based on golden hamsters Mesocricetus auratus . For the first time, we showed that O . felineus infection provokes time-dependent accumulation of oxidative hepatobiliary lesions in the injured liver of hamsters. In particular, over the course of infection, lipid peroxidation byproducts 4-hydroxynonenal and malondialdehyde were upregulated; these changes in general correlate with the dynamics of hepatic histopathological changes. We detected macrophages with various immunophenotypes and elevated levels of CD68, COX2, and CD163 in the O . felineus –infected animals. Meanwhile, there was direct time-dependent elevation of TNF-α (R = 0.79; p < 0.001) and CD163 protein levels (R = 0.58; p = 0.022). We also provide quantitative data about epithelial hyperplasia marker CK7 and a marker of myofibroblast activation (α smooth muscle actin). Our present data provide first insights into the histopathological mechanism by which O . felineus infection causes liver injuries. These findings support the inclusion of O . felineus in Group 1 of biological carcinogens.

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Secretion of Thioredoxin Peroxidase Protein of Cat Liver Fluke Opisthorchis felineus during Modeling of Experimental Opisthorchiasis

Cited by 9 publications

References 11 publications

Similarities and differences among the Opisthorchiidae liver flukes: insights from Opisthorchis felineus

Similarities and differences among the Opisthorchiidae liver flukes: insights from Opisthorchis felineus

Global changes in gene expression related to Opisthorchis felineus liver fluke infection reveal temporal heterogeneity of a mammalian host response

Opisthorchis felineus infection provokes time-dependent accumulation of oxidative hepatobiliary lesions in the injured hamster liver

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