Secretion of Prostaglandins E&lt;sub&gt;2&lt;/sub&gt; and F&lt;sub&gt;2&amp;alpha;&lt;/sub&gt; by the Bovine Endometrium in Response to Oxytocin during the Estrous Cycle

Miyamoto, Yuka; Skarżyński, Dariusz; Uenoyama, Yoshihisa; Okuda, Kiyoshi

doi:10.1262/jrd.44.289

Cited by 10 publications

(5 citation statements)

References 36 publications

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“…The intra‐ and interassay coefficients of variation (CVs) were on average 5.3% and 11.7%, respectively. The PGE 2 concentration in the culture medium was determined directly with HRP‐labelled PGE 2 (at a final dilution of 1 : 25 000) and anti‐PGE 2 serum (at a final dilution of 1 : 150 000) kindly donated by Dr. Seiji Ito, Kansai Medical University, Osaka, Japan, as described previously (Miyamoto et al. 1998).…”

Section: Methodsmentioning

confidence: 99%

Prostaglandin Endoperoxide Synthase 2 (PTGS2) and Prostaglandins F2α and E2 Synthases (PGFS and PGES) Expression and Prostaglandin F2α and E2 Secretion Following Oestrogen and/or Progesterone Stimulation of the Feline Endometrium

Siemieniuch

Jursza

Kowalewski

et al. 2012

Reprod Domestic Animals

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Sex steroids in synergy with prostaglandins (PG) are involved in the regulation of cyclic ovarian function. In this study, we investigated the mRNA expression of three genes involved in arachidonic acid (AA) metabolism and hence PG production in domestic cats: PG-endoperoxide synthase (PTGS2), PGF(2α) synthase (PGFS) and PGE(2) synthase (PGES). Feline endometria (n = 16) were collected at oestrus and mid and late phases of pseudopregnancy. In addition, the effects of E(2) and/or P(4) on PG secretion and gene expression on endometrial explants were studied in an in vitro culture system. Expression levels of all examined genes were up-regulated at the mid phase of pseudopregnancy. The effects of E(2) and/or P(4) treatment on both PG secretion and expression of the genes were observed after 12 h of culture. Expression of PGES was significantly up-regulated by E(2) plus P(4) at oestrus and the mid phase of pseudopregnancy and was also up-regulated by a single treatment with P(4) at late pseudopregnancy (p < 0.05). Simultaneous incubation with E(2) and P(4) up-regulated PTGS2 gene expression at oestrus and mid-luteal phase (p < 0.05). Progesterone plus E(2) significantly increased PGE(2) secretion at oestrus and the mid phase of pseudopregnancy. However, treatment with E(2) and/or P(4) affected neither PGF(2α) secretion nor PGFS expression at any phase after 12 h of culture. The overall findings indicate that genes involved in PG synthesis are up-regulated at the mid phase of pseudopregnancy. An increase in PGE(2) secretion and up-regulation of PGES and PTGS2 are the main responses of the endometrium to treatment with E(2) and P(4) at oestrus and the mid phase of pseudopregnancy in the cat. These data support the hypothesis that ovarian sex steroids via endometrial PGE(2) are involved in endocrine homoeostasis, especially at oestrus and the mid, but not the late, phase of pseudopregnancy in cats.

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Section: Methodsmentioning

confidence: 99%

Prostaglandin Endoperoxide Synthase 2 (PTGS2) and Prostaglandins F2α and E2 Synthases (PGFS and PGES) Expression and Prostaglandin F2α and E2 Secretion Following Oestrogen and/or Progesterone Stimulation of the Feline Endometrium

Siemieniuch

Jursza

Kowalewski

et al. 2012

Reprod Domestic Animals

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“…PGE 2 concentrations in the culture medium were determined with an enzyme immunoassay as described previously (Miyamoto et al. 1998).…”

Section: Methodsmentioning

confidence: 99%

Tumour Necrosis factor‐α Receptors are Present in the Corpus Luteum Throughout the Oestrous Cycle and During the Early Gestation Period in Pigs

Miyamoto

Sakumoto

Sakabe

et al. 2002

Reprod Domestic Animals

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To determine the physiological significance of tumour necrosis factor-alpha (TNFalpha) in the regulation of luteal functions in pig, this study was conducted to identify the presence of functional TNFalpha receptors in porcine corpora lutea (CL) throughout the oestrous cycle and the early gestation. The CL were isolated from pigs on days 4, 6, 8, 12 or 15 of the oestrous cycle (n=3; day 0 = oestrus) and days 15, 20 or 25 of gestation (n=3; day 0 = mating). A Scatchard analysis revealed the presence of a high-affinity binding site for TNFalpha in all samples (dissociation constant; 2.7 +/- 0.51 to 5.8 +/- 0.50 nM). The concentration of TNFalpha receptors was higher on day 15 of the oestrous cycle than on days 4 and 8 of the oestrous cycle (p < 0.05). Furthermore, TNFalpha receptor concentrations in the CL on days 15, 20 and 25 of gestation were significantly lower than on day 15 of the oestrous cycle (p< 0.05). On day 9 of the oestrous cycle, exposure of cultured luteal cells to 0.06-60 nM TNFalpha stimulated prostaglandin (PG) F2alpha and PGE2 secretion in a dose-dependent manner (p < 0.05). These results indicate that functional TNFalpha receptors are present in the porcine CL throughout the oestrous cycle and early gestation, and suggest that TNFalpha plays one or more physiological roles in regulating CL function throughout the oestrous cycle and the early gestation period. In addition, TNFalpha receptor concentration in the CL of the late luteal stage (day 15) of the oestrous cycle was higher than on the respective day in the early pregnant pig, suggesting that TNFalpha plays a role in accomplishing luteolysis in the porcine CL.

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“…The intra-and interassay coefficients of variation were on average 8.8% and 12.5%, respectively. PGE 2 concentrations were determined with an enzyme immunoassay using peroxidase-labeled PGE 2 and anti-PGE 2 serum as described previously [22]. Cross-reactivities of the anti-PGE 2 serum, validated by comparing the inhibition of binding of peroxidase-labeled PGE 2 to antiserum, were as follows: PGE 2 , 100%; PGE 1 , 18%; PGA 1 , 10%; PGA 2 , 4.6%; PGB 2 , 6.7%; PGD 2 , 0.13%; PGF 2␣ , 2.8%; PGJ 2 , 14%; and 15-keto PGE 2 , 0.05%.…”

Section: Enzyme Immunoassaymentioning

confidence: 99%

Tumor Necrosis Factor-α and Its Receptor in Bovine Corpus Luteum Throughout the Estrous Cycle1

Sakumoto

Berisha

Kawate

et al. 2000

Biology of Reproduction

Self Cite

102

114

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The objective of this study was to investigate tumor necrosis factor alpha (TNF-alpha) expression, the presence of functional TNF-alpha receptors, and expression of TNF receptor type I (TNF-RI) mRNA in the bovine corpus luteum (CL) during different stages of the estrous cycle. Reverse transcription (RT)-polymerase chain reaction (PCR) showed no difference in TNF-alpha mRNA expression during the estrous cycle. Concentrations of TNF-alpha in the CL tissue increased significantly from the mid to the late luteal stage and decreased thereafter (P < 0.05). An RT-PCR analysis showed higher levels of TNF-RI mRNA in CL of Days 3-7 than of other stages (P < 0.05). (125)I-TNF-alpha binding to the membranes of bovine CL was maximal after incubation at 38 degrees C for 48 h. The binding was much greater for TNF-alpha than for related peptides. A Scatchard analysis revealed the presence of a high-affinity binding site in the CL membranes collected at each phase of the estrous cycle (dissociation constant: 3.60 +/- 0.58-5.79 +/- 0.19 nM). In contrast to TNF-RI mRNA expression, the levels of receptor protein were similar at each stage of the estrous cycle. When cultured cells of all luteal stages were exposed to TNF-alpha (1-100 ng/ml), TNF-alpha stimulated prostaglandin F(2alpha) and prostaglandin E(2) secretion by the cells in a dose-dependent fashion (P < 0.01), especially during the early luteal phase, although it did not affect progesterone secretion. These results indicate the local production of TNF-alpha and the presence of functional TNF-RI in bovine CL throughout the estrous cycle, and suggest that TNF-alpha plays some roles in regulating bovine CL function throughout the estrous cycle.

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Secretion of Prostaglandins E<sub>2</sub> and F<sub>2α</sub> by the Bovine Endometrium in Response to Oxytocin during the Estrous Cycle

Cited by 10 publications

References 36 publications

Prostaglandin Endoperoxide Synthase 2 (PTGS2) and Prostaglandins F2α and E2 Synthases (PGFS and PGES) Expression and Prostaglandin F2α and E2 Secretion Following Oestrogen and/or Progesterone Stimulation of the Feline Endometrium

Prostaglandin Endoperoxide Synthase 2 (PTGS2) and Prostaglandins F2α and E2 Synthases (PGFS and PGES) Expression and Prostaglandin F2α and E2 Secretion Following Oestrogen and/or Progesterone Stimulation of the Feline Endometrium

Tumour Necrosis factor‐α Receptors are Present in the Corpus Luteum Throughout the Oestrous Cycle and During the Early Gestation Period in Pigs

Tumor Necrosis Factor-α and Its Receptor in Bovine Corpus Luteum Throughout the Estrous Cycle1

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