Secretion of prostaglandins and endothelin-1 by decidual endothelial cells from normal and preeclamptic pregnancies: Comparison with human umbilical vein endothelial cells

Gallery, Eileen D.M.; Rowe, Janet; Campbell, Suzanne; Hawkins, Therese

doi:10.1016/0002-9378(95)90649-5

Cited by 9 publications

(3 citation statements)

References 14 publications

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“…Finally, we would advise caution in the use of umbilical vein endothelial cells as controls. We found a much higher mediator release in these cells than in cells from healthy saphenous veins, as also shown previously in a comparison between human late pregnancy decidual and umbilical vein endothelial cells (Gallery et al , 1995). This casts doubt on the validity of their widespread use as a general surrogate for endothlial cells.…”

Section: Discussionsupporting

confidence: 91%

Imbalance between the endothelial cell‐derived contracting factors prostacyclin and angiotensin II and nitric oxide/cyclic GMP in human primary varicosis

Schuller‐Petrovič

Siedler

Kern

et al. 1997

British J Pharmacology

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1 The role of the endothelium in the vasomotor control of human veins in the lower extremity is little understood. We tested the hypothesis that the production of relaxing and contracting factors is altered in endothelial cells from varicose saphenous veins which may predispose to the decreased vessel tone observed in primary varicosis. 2 We determined the intracellular accumulation of guanosine 3':5'-cyclic monophosphate cyclic GMP; a measure of nitric oxide production) and the release of endothelin and prostacyclin (measured as its stable metabolite 6-keto-prostaglandin F 1a ) from cultured cells derived from the long saphenous veins of patients with primary varicosis (Varicose saphena group, n=27) or from patients undergoing coronary artery bypass surgery (Healthy saphena group, n=22). In addition, levels of endothelin, angiotensin II, bradykinin, cyclic GMP and cyclic AMP in plasma from patients with primary varicosis and healthy volunteers (n=8 ± 11 in each group) were determined. 3 Although basal cyclic GMP levels were similar, more cyclic GMP accumulated in response to histamine (1 ± 100 mmol l 71 ) in cells from varicose saphenous veins (0.75+0.1 pmol per well) than in cells from veins without varicosis (0.27+0.05 pmol per well). Furthermore, the relaxant potency of nitroprusside (1 nmol l 71 ± 300 mmol l 71 ) in vitro was higher for varicose veins (mean EC 50 =5.9 m mol l 71 ; n=8) than healthy veins (mean EC 50 =20.0 mmol l 71 ; n=7). 4 The production of prostacyclin was signi®cantly less in cells from varicose than healthy saphenous veins (66+8.7 and 121+20.1 nmol g 71 protein), but the production of endothelin was similar in both groups. Prostacyclin (3 nmol l 71 ± 30 mmol l 71 ) consistently contracted rings of varicose saphenous vein in vitro with a mean EC 50 value of 10 ± 20 mmol l 71 (n=7); the maximum tension generated was *50% of that of a completely depolarizing solution of K + (120 mmol l 71 ). 5 In plasma from patients with varicose veins, levels of cyclic GMP were higher than in healthy controls (9.2+0.03 and 7.2+0.02 nmol l 71 ), levels of angiotensin II were lower (81+11.5 and 147+21.7 pmol l 71 ), and levels of endothelin, cyclic AMP, and bradykinin were not di erent. 6 It is concluded that endothelial cells from diseased saphenous veins secrete less constrictor mediators than cells from healthy veins and that in diseased veins the nitric oxide/cyclic GMP system is upregulated which may shift the balance of vasoactive factors towards vasodilatation and contribute to the development of primary varicosis.

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Section: Discussionsupporting

confidence: 91%

Imbalance between the endothelial cell‐derived contracting factors prostacyclin and angiotensin II and nitric oxide/cyclic GMP in human primary varicosis

Schuller‐Petrovič

Siedler

Kern

et al. 1997

British J Pharmacology

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“…Using our in vitro assays of HUVE cells exposed to pregnancy plasma and serum, we and others have demonstrated an increase in HUVE cell PGI 2 production following acute exposure to these blood products. 26,126,127 More recent studies using HPLC and thin-layer chromatographic separation of [ 3 H]arachidonic acid metabolites indicate that the predominant eicosanoid synthesized by HUVE cells in the presence of human pregnancy plasma is the vasoconstrictor PGF 2a . 14 Greater production of PGF 2α was observed after exposure to preeclampsia plasma compared with matched, normal preg nancy plasma.…”

Section: Very Low Density Lipoproteins (Vldl) and Prostaglandinsmentioning

confidence: 99%

Circulating Factors as Markers and Mediators of Endothelial Cell Dysfunction in Preeclampsia

Taylor¹,

Groot²,

Cho³

et al. 1998

Semin Reprod Med

130

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During the past decade a new hypothesis has been formulated that explains many of the disparate findings associated with the pregnancy syndrome preeclampsia. With an increased awareness of the physiological significance of vascular endothelial cell function, the seemingly unrelated signs of hypertension, proteinuria, edema, and hypercoagulability have converged to provide clinical evidence of a unifying pathophysiological mechanism: systemic, maternal endothelial cell dysfunction. Investigators have attempted to test this hypothesis through two approaches. The first approach involves the identification of in vivo markers of vascular endothelial cell injury in women with clinically evident preeclampsia. The second approach focuses on the ability of circulating factors derived from the serum or plasma of patients afflicted with preeclampsia to perturb endothelial cell function in vitro. In this review we summarize the increasingly compelling evidence that maternal vascular endothelial cells are a critical target for toxic humoral activities that precipitate the multifaceted preeclampsia syndrome.

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“…This may also be the case in the umbilical vasculature, since both cultured endothelial cells [3] and cultured vascular smooth muscle cells [4] from human umbilical veins re-lins and acts as a highly selective ETB receptor agonist [14] . S6c induced no contractile response in six out of eight endothelium-denuded ring preparations of human umbilical veins, whereas the remaining two preparations contracted even at a low concentration of the agonist.…”

Section: Introductionmentioning

confidence: 99%

Endothelin B Receptors on Vascular Smooth Muscle Cells of the Human Umbilical Vein Mediate Vasoconstriction

Mildenberger¹,

Biesel²,

Siegel

et al. 2008

Fetal Diagn Ther

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Objective: To test the hypothesis that smooth muscle cells of the human umbilical vein have vasoconstricting endothelin B (ETB) receptors. Methods: In strip preparations of human umbilical veins isometric tension after exposure to the selective ETB receptor agonist sarafotoxin S6c (S6c) was compared to the tension before S6c exposure (set as 100%). Results: In intact preparations S6c induced vasoconstriction only at the highest concentration applied (10^–8M; 149.5 ± 12.5 vs. 100%, p < 0.05). In contrast, in endothelium-denuded preparations S6c induced vasoconstriction already at the lowest S6c concentration investigated (10^–11M; 111.7 ± 4.3 vs. 100%, p < 0.05). Conclusions: The vasoconstricting effect of S6c in endothelium-denuded human umbilical vein preparations points to smooth muscle cell ETB receptor activation.

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Secretion of prostaglandins and endothelin-1 by decidual endothelial cells from normal and preeclamptic pregnancies: Comparison with human umbilical vein endothelial cells

Cited by 9 publications

References 14 publications

Imbalance between the endothelial cell‐derived contracting factors prostacyclin and angiotensin II and nitric oxide/cyclic GMP in human primary varicosis

Imbalance between the endothelial cell‐derived contracting factors prostacyclin and angiotensin II and nitric oxide/cyclic GMP in human primary varicosis

Circulating Factors as Markers and Mediators of Endothelial Cell Dysfunction in Preeclampsia

Endothelin B Receptors on Vascular Smooth Muscle Cells of the Human Umbilical Vein Mediate Vasoconstriction

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