Secretion of beta-propeller phytase from tobacco and Arabidopsis roots enhances phosphorus utilization

Lung, Shiu‐Cheung; Chan, Wing-Lee; Yip, Wing Ho; Wang, Ling-Jian; Yeung, Edward C.; Lim, Boon Leong

doi:10.1016/j.plantsci.2005.03.006

Cited by 81 publications

(57 citation statements)

References 40 publications

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“…From Bacillus subtilis, a beta-propeller phytase was constitutively expressed in tobacco and Arabidopsis. Phytase activities in leaf and root extracts in transgenic tobacco, were 7 to 9-fold higher than those in wild-type and 4 to 6-fold higher extracellular phytase activity had been recorded in transgenic plants (Lung et al 2005;Singh et al 2011). …”

Section: Application Of Phytasementioning

confidence: 93%

Reduction of phytic acid and enhancement of bioavailable micronutrients in food grains

2013

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More than half of the world populations are affected by micronutrient malnutrition and one third of world's population suffers from anemia and zinc deficiency, particularly in developing countries. Iron and zinc deficiencies are the major health problems worldwide. Phytic acid is the major storage form of phosphorous in cereals, legumes, oil seeds and nuts. Phytic acid is known as a food inhibitor which chelates micronutrient and prevents it to be bioavailabe for monogastric animals, including humans, because they lack enzyme phytase in their digestive tract. Several methods have been developed to reduce the phytic acid content in food and improve the nutritional value of cereal which becomes poor due to such antinutrient. These include genetic improvement as well as several pre-treatment methods such as fermentation, soaking, germination and enzymatic treatment of grains with phytase enzyme. Biofortification of staple crops using modern biotechnological techniques can potentially help in alleviating malnutrition in developing countries.

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Section: Application Of Phytasementioning

confidence: 93%

Reduction of phytic acid and enhancement of bioavailable micronutrients in food grains

2013

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“…168phyA from B. subtilis showed increased biomass (up to 2-fold) and higher number of flowers and fruits compared to the wild type when grown on phytate as the only source of phosphorus [72]. Similarly, expression of B. subtilis 168PhyA phytase in Arabidopsis thaliana led to a higher shoot dry weight and an increase in phosphorus content by 100% compared to the wild type [75].…”

Section: Transgenic Plants As a Promising Alternative To Phosphate Fementioning

confidence: 97%

Microbial Phytases and Phytate: Exploring Opportunities for Sustainable Phosphorus Management in Agriculture

Balaban¹,

Suleimanova²,

Valeeva³

et al. 2017

AJMB

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Myo-inositol phosphates (phytates) are important biological molecules produced largely by plants to store phosphorus. Phytate is very abundant in many different soils making up a large portion of all soil phosphorus. This review assesses current phytase science from the perspective of its substrate, phytate, by examining the intricate relationship between the phytate-hydrolyzing enzymes and phytate as their substrate. Specifically, we examine available data on phytate's structural features, distribution in nature and functional roles. The role of phytases and their localization in soil and plant tissues are evaluated. We provide a summary of the current biotechnological advances in using industrial or recombinant phytases to improve plant growth and animal nutrition. The prospects of future discovery of novel phytases with improved biochemical properties and bioengineering of existing enzymes are also discussed. Two alternative but complementary directions to increase phosphorus bioavailability through the more efficient utilization of soil phytate are currently being developed. These approaches take advantage of microbial phytases secreted into rhizosphere either by phytase-producing microbes (biofertilizers) or by genetically engineered plants. More research on phytate metabolism in soils and plants is needed to promote environmentally friendly, more productive and sustainable agriculture.

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“…But the studies to date have been tested only in model plant species under sterilized conditions. For example, overexpression of phytase genes from a soil fungus (Aspergillus niger) or a soil bacteria (Bacillus subtilis) in transgenic model plants (such as Arabidopsis and tobacco) significantly increased exudation of phytase from plant roots (Richardson et al, 2001;Mudge et al, 2003;George et al, 2005;Lung et al, 2005), and several studies where the plant purple APase genes such as MtPHY1 and MtPAP1 were expressed in transgenic Arabidopsis and increased phytase exudation was seen (Xiao et al, 2005(Xiao et al, , 2006. In this study, we transformed a plant APase gene with phytase activity (AtPAP15) into an agriculturally important crop, soybean, and showed significantly increased phytase secretion from soybean roots.…”

Section: Discussionmentioning

confidence: 99%

“…For soybean (Glycine max) hairy root transformation, the 35S cauliflower mosaic virus (CaMV) AtPAP15 cassette including a carrot (Daucus carota) extensin leader signal peptide (Lung et al, 2005) was subcloned into pCAMBIA3301 (www.cambia.org) with the bar gene as the selective marker and GUS as the reporter gene, to create pCAMBIA3301-sp-AtPAP15-GUS vector.…”

Section: Vector Constructionmentioning

confidence: 99%

Overexpressing AtPAP15 Enhances Phosphorus Efficiency in Soybean

et al. 2009

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Low phosphorus (P) availability is a major constraint to crop growth and production, including soybean (Glycine max), on a global scale. However, 50% to 80% of the total P in agricultural soils exists as organic phosphate, which is unavailable to plants unless hydrolyzed to release inorganic phosphate. One strategy for improving crop P nutrition is the enhanced activity of acid phosphatases (APases) to obtain or remobilize inorganic phosphate from organic P sources. In this study, we overexpressed an Arabidopsis (Arabidopsis thaliana) purple APase gene (AtPAP15) containing a carrot (Daucus carota) extracellular targeting peptide in soybean hairy roots and found that the APase activity was increased by 1.5-fold in transgenic hairy roots. We subsequently transformed soybean plants with AtPAP15 and studied three homozygous overexpression lines of AtPAP15. The three transgenic lines exhibited significantly improved P efficiency with 117.8%, 56.5%, and 57.8% increases in plant dry weight, and 90.1%, 18.2%, and 62.6% increases in plant P content, respectively, as compared with wild-type plants grown on sand culture containing phytate as the sole P source. The transgenic soybean lines also exhibited a significant level of APase and phytase activity in leaves and root exudates, respectively. Furthermore, the transgenic lines exhibited improved yields when grown on acid soils, with 35.9%, 41.0%, and 59.0% increases in pod number per plant, and 46.0%, 48.3%, and 66.7% increases in seed number per plant. Taken together, to our knowledge, our study is the first report on the improvement of P efficiency in soybean through constitutive expression of a plant APase gene. These findings could have significant implications for improving crop yield on soils low in available P, which is a serious agricultural limitation worldwide.

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Secretion of beta-propeller phytase from tobacco and Arabidopsis roots enhances phosphorus utilization

Cited by 81 publications

References 40 publications

Reduction of phytic acid and enhancement of bioavailable micronutrients in food grains

Reduction of phytic acid and enhancement of bioavailable micronutrients in food grains

Microbial Phytases and Phytate: Exploring Opportunities for Sustainable Phosphorus Management in Agriculture

Overexpressing AtPAP15 Enhances Phosphorus Efficiency in Soybean

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