Secretins revealed: structural insights into the giant gated outer membrane portals of bacteria

Majewski, D.D.; Worrall, L.J.; Strynadka, N.C.J.

doi:10.1016/j.sbi.2018.02.008

Cited by 38 publications

(45 citation statements)

References 66 publications

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“…The secretin amphipathic helix lip (AHL) is thought to be a key determinant for 248 secretin outer membrane insertion and among Type IV pilus secretins, the AHL is 249 conserved 18 . The AHL is thought to mark the lower boundary of the outer 250 membrane region of secretins 18,29,30 . The upper boundary of the outer membrane is less clear since the micelle density in cryoEM reconstructions does not always 252 match the positions of aromatic residues 25 .…”

Section: The Putative Outer Membrane Region Of Vcpilq Is Thicker Thanmentioning

confidence: 99%

CryoEM structure of the Vibrio cholerae Type IV competence pilus secretin PilQ

Weaver

Sazinsky

Dalia

et al. 2020

Preprint

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Section: The Putative Outer Membrane Region Of Vcpilq Is Thicker Thanmentioning

confidence: 99%

CryoEM structure of the Vibrio cholerae Type IV competence pilus secretin PilQ

Weaver

Sazinsky

Dalia

et al. 2020

Preprint

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“…Understanding the instrinsic oligomeric states of protein subassemblies of the bacterial T2SS is necessary for deciphering the architectures and assembly principles of the functional T2SS apparatus. With the exception of the outer membrane secretin [34][35][36], experimental data on the oligomeric state of the membrane-embedded components of the T2SS is scarce. Therefore, we addressed the question of the oligomeric state of the purified GspLM complex via SEC-MALLS.…”

Section: Gsplm Is a Dimer Of Dimers In Solutionmentioning

confidence: 99%

The GspLM inner membrane complex from the bacterial type II secretion system is a dimer of dimers and interacts with the system ATPase with high affinity

Fulara

Ramou

Savvides

2020

Preprint

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ABSTACTThe type II secretion system (T2SS) is a multiprotein machinery spanning the diderm of gram-negative bacteria. T2SS contributes to the virulence of numerous gram-negative pathogens, including the multidrug resistant species Pseudomonas aeruginosa, Acinetobacter baumanii, Klebsiella pneumonia and Vibrio cholerae. Even though the T2SS has been studied extensively over the past three decades, our understanding of the molecular basis of its biogenesis and of its overall structure still remains unclear. Here we show that the core component of the inner membrane platform, the GspLM membrane protein complex, can be isolated as a dimer of dimers. Importantly, the complex is able to bind the T2SS ATPase, GspE, with high affinity. Finally, we have developed single domain VHH camelid antibodies (nanobodies) against the GspLM complex and have identified a nanobody that effectively prevents the cytoplasmic domain of GspL, GspLcyto, from binding to GspE. Our findings suggest that the T2SS ATPase is permanently associated with the inner membrane platform and that the GspELM complex should be considered as a key subassembly for the biogenesis of the T2SS apparatus.

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“…The cell wall of Gram-negative bacteria on the other hand is thinner but more complex and contains various polysaccharides, proteins and lipids beside peptidoglycan. The cell wall of Gram-negative bacteria also has an outer membrane, which constitutes the outer surface of the wall [22]. The antimicrobial effect of shellac occurred without migration of active agents.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Enhancement of water resistance and antimicrobial properties of paper sheets by coating with shellac

2019

Lett Appl NanoBioSci

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The present study was focused on the effect of shellac as a natural polymer on the mechanical, physical, and antimicrobial properties of the paper sheets. The surface modification of paper sheets was carried out using different shellac solutions. The capability of the treated sheet to absorb water and to permit air follow was studied by Cobb test and air permeability, respectively. It was found that the treated sheet had little affinity for water and low air permeability. The change of sheet surface properties after modification was ascertained by scanning electron microscopy and Fourier Transform Infrared Spectrophotometer (FT-IR). The shellac treated paper sheet displayed improved mechanical properties when compared with untreated paper sheet. The shellac treated sheets exhibit efficient antimicrobial activity against Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans. Consequently, the prepared paper sheet can be used as novel materials for packaging applications.

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Secretins revealed: structural insights into the giant gated outer membrane portals of bacteria

Cited by 38 publications

References 66 publications

CryoEM structure of the Vibrio cholerae Type IV competence pilus secretin PilQ

CryoEM structure of the Vibrio cholerae Type IV competence pilus secretin PilQ

The GspLM inner membrane complex from the bacterial type II secretion system is a dimer of dimers and interacts with the system ATPase with high affinity

Enhancement of water resistance and antimicrobial properties of paper sheets by coating with shellac

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