Secreted Proteomes of Different Developmental Stages of the Gastrointestinal Nematode Nippostrongylus brasiliensis

Sotillo, Javier; Sánchez-Flores, Alejandro; Cantacessi, Cinzia; Harcus, Yvonne; Pickering, Darren; Bouchery, Tiffany; Camberis, Mali; Tang, Shiau Choot; Giacomin, Paul; Mulvenna, Jason; Mitreva, Makedonka; Berriman, Matthew; Gros, Graham Le; Maizels, Rick M.; Loukas, Alex

doi:10.1074/mcp.m114.038950

Cited by 89 publications

(91 citation statements)

References 84 publications

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“…Twenty μg of protein was applied onto a denaturing polyacrylamide gel and subjected to electrophoresis. Protein reduction, alkylation and in gel digestion were performed as described previously [47]. Briefly, protein samples were reduced by addition of 0.5 M dithiothreitol at 65°C for 1 h, and then cysteine residues were alkylated with 0.5 M iodoacetamide (Sigma-Aldrich, St. Louis, MO) in darkness at 37°C for 45 min.…”

Section: Methodsmentioning

confidence: 99%

Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini

Suwannatrai

Tabsripair

et al. 2016

PLoS Negl Trop Dis

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Bithynia siamensis goniomphalos is a freshwater snail that serves as the first intermediate host of the human liver fluke Opisthorchis viverrini. This parasite is a major public health problem in different countries throughout the Greater Mekong sub-region (Thailand, southern Vietnam, Lao PDR and Cambodia). Chronic O. viverrini infection also results in a gradual increase of fibrotic tissues in the biliary tract that are associated with hepatobiliary diseases and contribute to cholangiocarcinoma (a fatal type of bile duct cancer). Infectivity of the parasite in the snail host is strongly correlated with destruction of helminths by the snail’s innate immune system, composed of cellular (hemocyte) and humoral (plasma) defense factors. To better understand this important host-parasite interface we applied sequential window acquisition of all theoretical spectra mass spectrometry (SWATH-MS) to identify and quantify the proteins from the hemolymph of B. siamensis goniomphalos experimentally infected with O. viverrini and compare them to non-infected snails (control group). A total of 362 and 242 proteins were identified in the hemocytes and plasma, respectively. Of these, 145 and 117 proteins exhibited significant differences in expression upon fluke infection in hemocytes and plasma, respectively. Among the proteins with significantly different expression patterns, we found proteins related to immune response (up-regulated in both hemocyte and plasma of infected snails) and proteins belonging to the structural and motor group (mostly down-regulated in hemocytes but up-regulated in plasma of infected snails). The proteins identified and quantified in this work will provide important information for the understanding of the factors involved in snail defense against O. viverrini and might facilitate the development of new strategies to control O. viverrini infection in endemic areas.

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Section: Methodsmentioning

confidence: 99%

Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini

Suwannatrai

Tabsripair

et al. 2016

PLoS Negl Trop Dis

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“…Astacins are a family of metallopeptidases highly abundant in the secretome of helminths . Indeed, the human hookworm N. americanus has 82 astacin‐encoding genes, and astacins are abundantly represented in ESPs of the rat hookworm Nippostrongylus brasiliensis and other nematodes . The role of astacins is not fully understood, although, in nematodes, they have been shown to participate in host invasion and parasite development .…”

Section: Top 20 Proteins Found By Mascot In the Excretory/secretory Pmentioning

confidence: 99%

Revisiting the Ancylostoma Caninum Secretome Provides New Information on Hookworm–Host Interactions

et al. 2017

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Hookworm infection is a major tropical parasitic disease affecting almost 500 million people worldwide. These soil-transmitted helminths can survive for many years in the intestine of the host, where they feed on blood, causing iron deficiency anemia and other complications. These parasites release a variety of molecules known as excretory/secretory products (ESPs) that are involved in many different biological processes that govern parasite survival. Using a combination of separation techniques such as SDS-PAGE and OFFGEL electrophoresis, in combination with state-of-the-art mass spectrometry we have reanalyzed the dog hookworm, Ancylostoma caninum, ESPs (AcESP). We identified 315 proteins present in the AcESP, compared with just 105 identified in previous studies. The most highly represented family of proteins is the SCP/TAPs (110 of the 315 proteins), and the most abundant constituents of AcESP are homologues of the tissue inhibitors of metalloproteases (TIMP) family. Interestingly, we identified new homologs of well-known vaccine candidates and immunomodulatory proteins. This study provides novel information about the proteins secreted by A. caninum, and constitutes a comprehensive dataset to study the proteins involved in host-hookworm interactions.

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“…These data demonstrate that the ability to increase selenoprotein expression in monocytes/macrophages via dietary supplementation with selenium can be potentially harnessed to impact hostpathogen interaction. Comparative proteomic analysis of infective larval (L3) and adult worm stages of N. brasiliensis indicated the expression of a group of antioxidant enzymes, including the protein disulfide oxidoreductase (most likely a thioredoxin reductase), protein disulfide isomerase, peroxiredoxin, superoxide dismutase (Cu/Zn), and thioredoxin-like proteins (46). Thus, it appears that the larvae and/or adult worms may be well positioned to maintain infectivity and fecundity even under selenium-deficient conditions.…”

Section: Ccr3mentioning

confidence: 99%

Selenoprotein Expression in Macrophages Is Critical for Optimal Clearance of Parasitic Helminth Nippostrongylus brasiliensis

Nelson

Shay

James

et al. 2016

Journal of Biological Chemistry

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The plasticity of macrophages is evident in helminthic parasite infections, providing protection from inflammation. Previously we demonstrated that the micronutrient selenium induces a phenotypic switch in macrophage activation from a classically activated (pro-inflammatory; M1/CAM) toward an alternatively activated (anti-inflammatory; M2/AAM) phenotype, where cyclooxygenase (COX)-dependent cyclopentenone prostaglandin J 2 (15d-PGJ 2 ) plays a key role. Here, we hypothesize that dietary selenium modulates macrophage polarization toward an AAM phenotype to assist in the increasing clearance of adult Nippostrongylus brasiliensis, a gastrointestinal nematode parasite. Mice on a selenium-adequate (0.08 ppm) diet significantly augmented intestinal AAM presence while decreasing adult worms and fecal egg production when compared with infection of mice on selenium-deficient (<0.01 ppm) diet.

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Secreted Proteomes of Different Developmental Stages of the Gastrointestinal Nematode Nippostrongylus brasiliensis

Cited by 89 publications

References 84 publications

Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini

Differential Protein Expression in the Hemolymph of Bithynia siamensis goniomphalos Infected with Opisthorchis viverrini

Revisiting the Ancylostoma Caninum Secretome Provides New Information on Hookworm–Host Interactions

Selenoprotein Expression in Macrophages Is Critical for Optimal Clearance of Parasitic Helminth Nippostrongylus brasiliensis

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