Secreted proteins of <i>Uromyces fabae</i>: similarities and stage specificity

Link, Tobias; Voegele, Ralf T.

doi:10.1111/j.1364-3703.2007.00448.x

Cited by 41 publications

(51 citation statements)

References 42 publications

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“…NCU04493 and its paralog NCU05667 (acw-3) encode predicted secreted proteins with simi- larity to fungal proteins that are anchored in the cell wall (GPI anchors). The functions of these proteins in the Ascomycota are unclear, but in some Basidiomycota, they appear to be involved in both fruiting body formation and infection cell development (Link and Voegele 2008). One member of this family from the Shitake mushroom interacts with a fruiting body formation-induced MAP kinase in the yeast two-hybrid system (Szeto et al 2007).…”

Section: Resultsmentioning

confidence: 99%

Temporal and Spatial Regulation of Gene Expression During Asexual Development ofNeurospora crassa

et al. 2010

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In this study we profiled spatial and temporal transcriptional changes during asexual sporulation in the filamentous fungus Neurospora crassa. Aerial tissue was separated from the mycelium to allow detection of genes specific to each tissue. We identified 2641 genes that were differentially expressed during development, which represents 25% of the predicted genes in the genome of this model fungus. On the basis of the distribution of functional annotations of 1102 of these genes, we identified gene expression patterns that define key physiological events during conidial development. Not surprisingly, genes encoding transcription factors, cell wall remodeling proteins, and proteins involved in signal transduction were differentially regulated during asexual development. Among the genes differentially expressed in aerial tissues the majority were unclassified and tended to be unique to ascomycete genomes. This finding is consistent with the view that these genes evolved for asexual development in the Pezizomycotina. Strains containing deletions of several differentially expressed genes encoding transcription factors exhibited asexual development-associated phenotypes. Gene expression patterns during asexual development suggested that cAMP signaling plays a critical role in the transition from aerial growth to proconidial chain formation. This observation prompted us to characterize a deletion of the gene encoding a highaffinity cAMP phosphodiesterase (NCU00478). NCU00478 was determined to be allelic to aconidiate-2, a previously identified genetic locus controlling conidiation.

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Section: Resultsmentioning

confidence: 99%

Temporal and Spatial Regulation of Gene Expression During Asexual Development ofNeurospora crassa

et al. 2010

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Section: Introductionmentioning

confidence: 99%

“…In contrast to this in silico approach, the 'signal sequence trap' is a yeast-based genetic screen for cDNAs that contain functional secretion signals, based on the complementation of a reporter gene lacking a signal peptide (Klein et al, 1996). Signal sequence trapping has been applied successfully to a wide range of eukaryotes, including two plant pathogens, Phytophthora sojae (Lee et al, 2006) and Uromyces fabae (Link & Voegele, 2008).In addition to their critical role in host penetration, we speculate that Colletotrichum appressoria, and the penetration pegs that emerge from them, secrete soluble effector proteins that permit the fungus to overcome host defence responses and reprogram host cells for biotrophy. As a first step towards the discovery of secreted effectors in C. higginsianum, we have generated expressed sequence tags (ESTs) from a stage-specific cDNA library prepared from mature appressoria grown in vitro.…”

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confidence: 99%

Identification of soluble secreted proteins from appressoria of Colletotrichum higginsianum by analysis of expressed sequence tags

et al. 2008

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The hemibiotrophic ascomycete Colletotrichum higginsianum causes anthracnose disease on brassica crops and the model plant Arabidopsis. Melanized appressoria pierce the host cuticle and cell wall to form specialized biotrophic hyphae inside living epidermal cells. To identify proteins secreted by appressoria that may function as virulence effectors, a cDNA library was prepared from mature appressoria formed in vitro. Bidirectional sequencing of 980 clones generated 1442 high-quality expressed sequence tags (ESTs), comprising 518 unique sequences. BLASTX analysis showed that 353 (68 %) of these had significant similarity to entries in the NCBI non-redundant protein database, of which 49 were also homologous to experimentally verified fungal pathogenicity genes. ORFs were predicted ab initio from the unique sequences and screened for potential signal peptides using SignalP. Fifty-three unique sequences (10 %) were predicted to encode proteins entering the secretory pathway, of which 26 were likely to be soluble secreted proteins. For a selected subset of these, RT-PCR showed that seven genes that encode secreted proteins of unknown function, including two Colletotrichum-specific genes, are upregulated in appressoria and expressed early during plant infection, and therefore represent candidate effectors. INTRODUCTIONThe ascomycete genus Colletotrichum comprises one of the most economically damaging groups of plant-pathogenic fungi, causing anthracnose diseases and blights on an enormous range of dicot and monocot crop plants in temperate, tropical and subtropical regions (Bailey & Jeger, 1992). The crucifer anthracnose pathogen Colletotrichum higginsianum has a wide host range, attacking many cultivated forms of Brassica and Raphanus as well as wild crucifers, including the model plant Arabidopsis thaliana (Narusaka et al., 2004;O'Connell et al., 2004). C. higginsianum employs a two-stage, hemibiotrophic infection strategy: after melanized appressoria breach the host cuticle and cell wall, the fungus initially grows biotrophically inside living epidermal cells, before entering a destructive necrotrophic phase in which host tissues are killed and extensively macerated by cell-wall-degrading enzymes. During the biotrophic phase, C. higginsianum differentiates specialized intracellular primary hyphae similar to haustoria, which expand and locally modify the host plasma membrane (Shimada et al., 2006). However, in contrast to obligately biotrophic pathogens of Arabidopsis, Colletotrichum can be cultured axenically and stably transformed using a variety of methods; it is also a haploid organism with uninucleate conidia, which facilitates mutational analyses. This pathosystem therefore provides an attractive model for studying biotrophy, in which both partners in the interaction are genetically tractable.Whole-genome sequencing projects have revealed that fungal and oomycete plant pathogens possess large repertoires of secreted proteins, which can play diverse roles in pathogenicity and interactions with host cells (Dean...

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“…It is based on the complementation of a reporter gene lacking a signal peptide (Klein et al 1996). This approach has been applied successfully to identify secreted proteins from P. sojae (Lee et al 2006), U. fabae (Link and Voegele 2008), and Colletotrichum graminicola (Krijger et al 2008).…”

Section: Secretomementioning

confidence: 99%

Proteomic studies of phytopathogenic fungi, oomycetes and their interactions with hosts

et al. 2009

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Proteomics, the systematic analysis of the proteome, is a powerful tool in the post-genomic era. Proteomics studies have examined global changes in proteomes of phytopathogenic fungi, oomycetes and their hosts during compatible or incompatible interactions. This article compiles proteomics reports in order to decipher the molecular mechanisms underlying fungal development (infection-related morphogenesis), fungal or oomycete-host plant interactions, and phytopathogenesis.

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Secreted proteins of Uromyces fabae: similarities and stage specificity

Cited by 41 publications

References 42 publications

Temporal and Spatial Regulation of Gene Expression During Asexual Development ofNeurospora crassa

Temporal and Spatial Regulation of Gene Expression During Asexual Development ofNeurospora crassa

Identification of soluble secreted proteins from appressoria of Colletotrichum higginsianum by analysis of expressed sequence tags

Proteomic studies of phytopathogenic fungi, oomycetes and their interactions with hosts

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