Secreted protein acidic and rich in cysteine (SPARC) regulates thermogenesis in white and brown adipocytes

Mukherjee, Sulagna; Choi, Min Ji; Kim, Sang Woo; Yun, Jong Won

doi:10.1016/j.mce.2020.110757

Cited by 20 publications

(8 citation statements)

References 60 publications

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“…Overexpression of SPARC modulates the expression levels of various pro-inflammatory cytokines, critically involved in insulin resistance, glucose and lipid metabolism during adipogenesis (Shen et al, 2014). Moreover, SPARC regulated thermogenesis in white and brown adipocytes, directly interacting with VEGF in adipocytes (Mukherjee et al, 2020). Recently, deletion of SPARC expression has been reported to cause diabetes mellitus in mice, demonstrating that SPARC deficient mice represent a reliable model for obesity and its metabolic complications, including diabetes mellitus (Atorrasagasti et al, 2019).…”

Section: Sparc Obesity and Metabolic Dysfunctionmentioning

confidence: 99%

Adipokines: New Potential Therapeutic Target for Obesity and Metabolic, Rheumatic, and Cardiovascular Diseases

et al. 2020

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Besides its role as an energy storage organ, adipose tissue can be viewed as a dynamic and complex endocrine organ, which produces and secretes several adipokines, including hormones, cytokines, extracellular matrix (ECM) proteins, and growth and vasoactive factors. A wide body of evidence showed that adipokines play a critical role in various biological and physiological functions, among which feeding modulation, inflammatory and immune function, glucose and lipid metabolism, and blood pressure control. The aim of this review is to summarize the effects of several adipokines, including leptin, diponectin, resistin, chemerin, lipocalin-2 (LCN2), vaspin, omentin, follistatin-like 1 (FSTL1), secreted protein acidic and rich in cysteine (SPARC), secreted frizzled-related protein 5 (SFRP5), C1q/TNF-related proteins (CTRPs), family with sequence similarity to 19 member A5 (FAM19A5), wingless-type inducible signaling pathway protein-1 (WISP1), progranulin (PGRN), nesfatin-1 (nesfatin), visfatin/PBEF/NAMPT, apelin, retinol binding protein 4 (RPB4), and plasminogen activator inhibitor-1 (PAI-1) in the regulation of insulin resistance and vascular function, as well as many aspects of inflammation and immunity and their potential role in managing obesity-associated diseases, including metabolic, osteoarticular, and cardiovascular diseases.

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Section: Sparc Obesity and Metabolic Dysfunctionmentioning

confidence: 99%

Adipokines: New Potential Therapeutic Target for Obesity and Metabolic, Rheumatic, and Cardiovascular Diseases

et al. 2020

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“…Moreover, our previous in vitro study showed that adding or inducing SPARC in C2C12 muscle cells increased their differentiation, myogenin expression, collagen expression, and the expression of two mitochondrial proteins (UQCRC2 and SDHB) [13] and a mitochondrial biogenesis master regulator (PGC-1α) [8]. The reduced adiposity in Tg mice correlated with the property SPARC had on white AT and also on BAT towards improving their metabolic performance (lipolysis, fat oxidation, and browning in white adipocytes and BAT activation [58]). Similarly, our previous [11] and current in vivo results indicated that whereas exercise improved muscle (TA) mass, grip power (trend), glucose tolerance, collagen expression (trend), glucose uptake (GLUT4 expression), and mitochondrial oxidative phosphorylation (MT-CO1 expression), it reduced adiposity and body weight (trend).…”

Section: Sparc Overexpression Mimics Exercise Benefitsmentioning

confidence: 85%

“…The property SPARC had on white AT, and also on BAT, towards improving their metabolic performance would also have consequences. Indeed, whereas SPARC induced lipolysis, fat oxidation, and browning in the white adipocytes and BAT activation, its knockdown reduced the markers of these metabolic pathways [58]. Thus, Sparc KO would be towards adiposity development resulting from a poor local metabolic rate of the adipose tissues.…”

Section: Sparc Ko To Optimize (Accelerated) Ageing Animal Modelsmentioning

confidence: 99%

Secreted Protein Acidic and Rich in Cysteine (Sparc) KO Leads to an Accelerated Ageing Phenotype Which Is Improved by Exercise Whereas SPARC Overexpression Mimics Exercise Effects in Mice

et al. 2022

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Secreted protein acidic and rich in cysteine (SPARC) is a matricellular glycoprotein implicated in various functions, including metabolism, tissue regeneration, and functional homeostasis. SPARC/Sparc declines with ageing but increases with exercise. We aim to verify two hypotheses: (1) SPARC deficiency leads to an ageing-like phenotype (metabolic decline, muscle loss, etc.), and (2) SPARC overexpression would mimic exercise, counteract ageing, and improve age-related changes. Our mice experiments are divided into two parts. First, we explore the consequences of Sparc knockout (KO) and compare them to the ageing effects. We also observe the effects of exercise. In the second part, we study the effects of SPARC overexpression and compare them to the exercise benefits. At the end, we make an analysis of the results to point out the analogies between Sparc KO and the ageing-like phenotype on the one hand and make comparisons between SPARC overexpression and exercise in the context of exercise counteracting ageing. The measurements were mainly related to tissue weights, adiposity, metabolism, and muscle strength. The main findings are that Sparc KO reduced glucose tolerance, muscle glucose transporter expression, and abdominal adipose tissue weight but increased glycogen content in the muscle. SPARC overexpression increased muscle strength, muscle mass, and expressions of the muscle glucose transporter and mitochondrial oxidative phosphorylation but lowered the glycemia and the adiposity, especially in males. Collectively, these findings, and the data we have previously reported, show that Sparc KO mice manifest an ageing-like phenotype, whereas SPARC overexpression and exercise generate similar benefits. The benefits are towards counteracting both the SPARC deficiency-induced ageing-like phenotype as well as reversing the age-related changes. The potential applications of these findings are to build/optimize Sparc KO-based animal models of various health conditions and, on the other hand, to develop therapies based on introducing SPARC or targeting SPARC-related pathways to mimic exercise against age-related and metabolic disorders.

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“…In this study, we observe that SPARC high expression phenotype was associated with TGF beta signaling pathway, pathways in cancer, Wnt signaling pathway, Mitogen-activated protein kinase (MAPK) signaling pathway, focal adhesion, cell adhesion molecules cams, melanogenesis and small cell lung cancer. TGF beta signaling pathway is instrumental in mammalian development which has pivotal role in many mechanisms of breast cancer [17] , lung cancer [18] and other cancer [19][20][21] . Wnt signaling pathway is required for adult tissue maintenance, and perturbations in Wnt signaling promote human cancer [22,23] .MAPK signaling pathway activated during the differentiation of myogenic cell lines [24] .…”

Section: Discussionmentioning

confidence: 99%

Diagnosis and Prognostic Value of SPARC in Gastric Carcinoma: Database Mining for GCTA

Ying

Jin

2020

Preprint

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Abstract Gastric carcinoma (GC) remains high incidence and mortality both in developed and developing countries. SPARC is extracellular non-structural matrix glycoprotein. Previous studies were closely associated with bone disease. However, the role of SPARC in GC remains largely unclear. In our study, we explored the diagnosis, prognosis and pathway enrichments value of SPARC in GC. Here, with the data from The Cancer Genome Atlas (TCGA), we used receiver operating characteristic (ROC) curve analysis to estimate the diagnosis value of the SPARC expression, Univariate and multivariate analysis to the prognosis, Gene set enrichment analysis (GSEA) to the signal pathway enrichments. As a result, SPARC expression was significantly higher in the GC tissue samples. Those with high SPARC expression of GC patients were worse prognosis. GSEA shows the gene sets related signal pathways including transforming growth factor (TGF) beta signaling pathway, pathways in cancer, Wnt signaling pathway, Mitogen-activated protein kinase (MAPK) signaling pathway etc. In brief, those results suggest that SPARC can serve as a potential biomarker for GC in diagnosis and prognosis.

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Secreted protein acidic and rich in cysteine (SPARC) regulates thermogenesis in white and brown adipocytes

Cited by 20 publications

References 60 publications

Adipokines: New Potential Therapeutic Target for Obesity and Metabolic, Rheumatic, and Cardiovascular Diseases

Adipokines: New Potential Therapeutic Target for Obesity and Metabolic, Rheumatic, and Cardiovascular Diseases

Secreted Protein Acidic and Rich in Cysteine (Sparc) KO Leads to an Accelerated Ageing Phenotype Which Is Improved by Exercise Whereas SPARC Overexpression Mimics Exercise Effects in Mice

Diagnosis and Prognostic Value of SPARC in Gastric Carcinoma: Database Mining for GCTA

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