Secreted Frizzled-Related Protein 1 Promotes Odontoblastic Differentiation and Reparative Dentin Formation in Dental Pulp Cells

Ipposhi, Keita; Tomokiyo, Atsushi; Ono, Taiga; Yamashita, Kozue; Alhasan, M Anas; Hasegawa, Daigaku; Yoshida, Shinichiro; Sugii, Hideki; Itoyama, Tomohiro; Ogawa, Makoto; Maeda, Hidefumi

doi:10.3390/cells10092491

Cited by 7 publications

(5 citation statements)

References 49 publications

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“…As for the molecular mechanisms underlying FABP4 suppression-induced pseudohypoxia in HOCF cells, in addition to HIF mechanisms, calcium-related signaling may be involved, as suggested by a previous study [ 33 ]. In support of this idea, IPA analysis indicated that (1) cell signaling was included in the top five molecular and cellular functions, (2) the calcium-binding protein S100-related signaling pathway was included in the top five canonical pathways and (3) upstream regulators NKX2-1 [ 34 ], HOXA10 [ 35 ], GATA2 [ 36 ] and CEBPA [ 37 ] and causal network master regulators NKX2-1 [ 34 ], TRPM2 [ 38 ] and SFRP1 [ 39 ] are known to regulate Ca 2+ signaling and/or mitochondrial functions.…”

Section: Discussionmentioning

confidence: 99%

FABP4 Is an Indispensable Factor for Regulating Cellular Metabolic Functions of the Human Retinal Choroid

Ohguro,

Watanabe,

Sato

et al. 2024

Bioengineering

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The purpose of the current study was to elucidate the physiological roles of intraocularly present fatty acid-binding protein 4 (FABP4). Using four representative intraocular tissue-derived cell types, including human non-pigmented ciliary epithelium (HNPCE) cells, retinoblastoma (RB) cells, adult retinal pigment epithelial19 (ARPE19) cells and human ocular choroidal fibroblast (HOCF) cells, the intraocular origins of FABP4 were determined by qPCR analysis, and the intracellular functions of FABP4 were investigated by seahorse cellular metabolic measurements and RNA sequencing analysis using a specific inhibitor for FABP4, BMS309403. Among these four different cell types, FABP4 was exclusively expressed in HOCF cells. In HOCF cells, both mitochondrial and glycolytic functions were significantly decreased to trace levels by BMS309403 in a dose-dependent manner. In the RNA sequencing analysis, 67 substantially up-regulated and 94 significantly down-regulated differentially expressed genes (DEGs) were identified in HOCF cells treated with BMS309403 and those not treated with BMS309403. The results of Gene Ontology enrichment analysis and ingenuity pathway analysis (IPA) revealed that the DEGs were most likely involved in G-alpha (i) signaling, cAMP-response element-binding protein (CREB) signaling in neurons, the S100 family signaling pathway, visual phototransduction and adrenergic receptor signaling. Furthermore, upstream analysis using IPA suggested that NKX2-1 (thyroid transcription factor1), HOXA10 (homeobox A10), GATA2 (gata2 protein), and CCAAT enhancer-binding protein A (CEBPA) were upstream regulators and that NKX homeobox-1 (NKX2-1), SFRP1 (Secreted frizzled-related protein 1) and TREM2 (triggering receptor expressed on myeloid cells 2) were causal network master regulators. The findings in this study suggest that intraocularly present FABP4 originates from the ocular choroid and may be a critical regulator for the cellular homeostasis of non-adipocyte HOCF cells.

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Section: Discussionmentioning

confidence: 99%

FABP4 Is an Indispensable Factor for Regulating Cellular Metabolic Functions of the Human Retinal Choroid

Ohguro,

Watanabe,

Sato

et al. 2024

Bioengineering

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“…Nano β-TCP/collagen scaffolds were used in this study. Our previous studies have reported that the nano β-TCP/collagen scaffolds containing with various proteins in direct pulp capping model promote reparative dentin formation 36,37 . The concentration of proteins added to the scaffolds in vivo was higher than that of proteins stimulating cells in vitro.…”

Section: Discussionmentioning

confidence: 99%

Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells

Fujino

Tomokiyo

Sugiura

et al. 2023

Sci Rep

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Conventional direct pulp-capping materials induce pulp cells to secrete various biomolecules in pulp tissues that promote reparative dentin formation through induction of odontoblastic differentiation of dental pulp stem cells (DPSCs). However, these biomolecules sometimes induce bone-like dentin with poor sealing properties. Therefore, exploration of biomolecules that allow tight sealing by tubular reparative dentin is required. We recently reported that dopamine (DA) is involved in dentinogenesis. Hence, we investigated the effect of DA on odontoblastic differentiation of DPSCs and reparative dentin formation. Both tyrosine hydroxylase (TH), a DA synthetase, and DA were expressed in odontoblast-like cells in vivo. In vitro, their expression was increased during odontoblastic differentiation of DPSCs. Furthermore, TH-overexpressing DPSCs had promoted odontoblastic differentiation and DA production. Moreover, DA stimulation promoted their differentiation and induced tubular reparative dentin. These results suggest that DA produced by TH is involved in odontoblastic differentiation of DPSCs and has an inductive capacity for reparative dentin formation similar to primary dentin. This study may lead to the development of therapy to preserve vital pulp tissues.

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“…The presence of SFRP1 in human milk was previously reported (Molinari et al., 2012), but as SFRP1 was not detected in previous research about bovine milk and colostrum (Le et al., 2011; Nissen et al., 2017; Zhang et al., 2015a, 2015b), it was detected in bovine colostrum (up‐regulation on high‐quality colostrums) for the first time in this study. As SFPR‐1 is known to regulate the growth and differentiation of certain cell types and to have antiproliferative effects on vascular cells (Bodine et al., 2009; Molinari et al., 2012, Ipposhi et al, 2021) it is thought that it may be similar to TETN, which plays a role in supporting high mitotic activity in newborns.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the differences in proteomics of high‐quality bovine colostrum and low‐quality bovine colostrum

Kaçar,

Batmaz

2023

Veterinary Medicine & Sci

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BackgroundAlthough there are studies on colostrum and milk proteomics of different species in the literature, there is no published report about different quality bovine colostrums’ proteomics.ObjectivesThe aim of this study was to compare the proteome content of high‐ and low‐quality bovine colostrums for the first time.MethodsColostrum samples were collected from 32 Holstein cows from the same farm that had just calved. Brix% levels of colostrums were measured, and then, those with a Brix% value of ≥27% were classified as high‐quality and those with a Brix% value of ˂22% as low‐quality. Three samples from high‐quality and low‐quality colostrums were selected and proteomic analyses were performed by pooling separately.ResultsTotally 95 proteins were identified in the colostrums, and 19 of them showed significant changes between high‐ and low‐quality colostrums. Expressions in colostrum of glycosylation‐dependent cell adhesion molecule‐1, cofilin‐1, alpha‐S2‐casein, alpha‐lactalbumin, alpha‐1B‐glycoprotein, actin_cytoplasmic‐1, nucleobindin‐1, cathelicidin‐4, inter‐alpha‐trypsin inhibitor heavy chain H4, chitinase‐3‐like protein 1 and monocyte differentiation antigen CD14 were lower, whereas tetranectin, secreted frizzled‐related protein‐1 (SFRP1), perilipin‐2, coatomer subunit epsilon (COPE), butyrophilin subfamily 1 member A1, polyubiquitin‐B, lactadherin and albumin levels were higher in high‐quality colostrum than low‐quality colostrum. Moreover, SFRP1, COPE and cathelicidin‐4 proteins were identified for the first time in bovine colostrum. In high‐quality colostrum, the most prominently down‐regulated proteins were cathelicidin‐4 (26.01‐fold) and cofilin‐1 (17.42‐fold), and the most prominently up‐regulated proteins were COPE (3.37‐fold) and tetranectin (3.07‐fold).ConclusionsIt was detected that the proteome contents of high‐ and low‐quality bovine colostrums were different from each other. As new functions are added to the protein databases regarding these proteins detected in colostrums, the interactions of proteins with each other and with other molecules will be detailed and the effects of high‐quality colostrums on passive transfer immunity and calf health will be understood in full detail.

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Secreted Frizzled-Related Protein 1 Promotes Odontoblastic Differentiation and Reparative Dentin Formation in Dental Pulp Cells

Cited by 7 publications

References 49 publications

FABP4 Is an Indispensable Factor for Regulating Cellular Metabolic Functions of the Human Retinal Choroid

FABP4 Is an Indispensable Factor for Regulating Cellular Metabolic Functions of the Human Retinal Choroid

Dopamine is involved in reparative dentin formation through odontoblastic differentiation of dental pulp stem cells

Evaluation of the differences in proteomics of high‐quality bovine colostrum and low‐quality bovine colostrum

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