Secreted enzymes of Aeromonas

Pemberton, John

doi:10.1016/s0378-1097(97)00186-9

Cited by 38 publications

(56 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A low yield as reported is probably due to difficulty of elimination of the lipopolysaccharide produced by the strain that is found to be strongly associated with the lipid hydrolysis [11]. The M. luteolum colony on trybutyrin agar was more viscous in nature and it may be due to the high lipopolysaccharide content of the bacterium [33]. In the present study, the yield of cold active lipase from M. luteolum is low after partial purification.…”

Section: Resultsmentioning

confidence: 40%

Extracellular cold-active lipase of Microbacterium luteolum isolated from Gangotri glacier, western Himalaya: Isolation, partial purification and characterization

Joseph

Shrivastava

Ramteke

2012

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 40%

Extracellular cold-active lipase of Microbacterium luteolum isolated from Gangotri glacier, western Himalaya: Isolation, partial purification and characterization

Joseph

Shrivastava

Ramteke

2012

Journal of Genetic Engineering and Biotechnology

View full text Add to dashboard Cite

“…The gene ascF-ascG encodes the needle complex and a chaperone, respectively (25). It is recognized that gcat has lipase or phospholipase activity, which mediate erythrocyte lysis by digesting their plasma membrane (54). …”

Section: Resultsmentioning

confidence: 99%

Virulence potential and antibiotic susceptibility pattern of motile aeromonads associated with freshwater ornamental fish culture systems: a possible threat to public health

Sreedharan¹,

Philip²,

Singh³

2012

Braz. J. Microbiol.

View full text Add to dashboard Cite

Aeromonas spp. are ubiquitous aquatic organisms, associated with multitude of diseases in several species of animals, including fishes and humans. In the present study, water samples from two ornamental fish culture systems were analyzed for the presence of Aeromonas. Nutrient agar was used for Aeromonas isolation, and colonies (60 No) were identified through biochemical characterization. Seven clusters could be generated based on phenotypic characters, analyzed by the programme NTSYSpc, Version 2.02i, and identified as: Aeromonas caviae (33.3%), A. jandaei (38.3%) and A. veronii biovar sobria (28.3%). The strains isolated produced highly active hydrolytic enzymes, haemolytic activity and slime formation in varying proportions. The isolates were also tested for the enterotoxin genes (act, alt and ast), haemolytic toxins (hlyA and aerA), involved in type 3 secretion system (TTSS: ascV, aexT, aopP, aopO, ascF-ascG, and aopH), and glycerophospholipid-cholesterol acyltransferase (gcat). All isolates were found to be associated with at least one virulent gene. Moreover, they were resistant to frequently used antibiotics for human infections. The study demonstrates the pathogenic potential of Aeromonas, associated with ornamental fish culture systems suggesting the emerging threat to public health.

show abstract

“…three different types of proteases have been identified: a temperature-labile serine-protease and two metalloproteases, both temperature-stable but EDTA (ethylenediaminetetraacetic acid)-sensitive and -insensitive, respectively. Additionally, aminopeptidases with a number of specific activities have been described: catabolism of exogenously supplied peptides, extracellular activation of aerolysine, or cleavage of amino-terminal methionine from newly synthesized peptide chains (methionin aminopeptidase) [134]. …”

Section: Exotoxins and Other Extracellular Enzymesmentioning

confidence: 99%

“…They may provide nutrients and constitute virulence factors by interacting with human leukocytes or by affecting several immune systems functions through free fatty acids generated by lipolytic activity. In A. hydrophila different lipases, such as the Ah65 lipase/acyltransferase, H3, Apl1 and Lip, have been described [134, 135], with the Apl1 lipase showing phospholipase C activity. In Aeromonas spp.serogroup O: 34 two lipases have been described: phospholipase A1 and C. The phospholipase C shows lecithinase and cytotoxic activities and its role as a virulence factor has been demonstrated [136].…”

Section: Exotoxins and Other Extracellular Enzymesmentioning

confidence: 99%

“…In Aeromonas spp.serogroup O: 34 two lipases have been described: phospholipase A1 and C. The phospholipase C shows lecithinase and cytotoxic activities and its role as a virulence factor has been demonstrated [136]. Furthermore, glycerophospholipid-cholesterol acyltransferases (GCAT), which digest erythrocytes membranes and leads their lysis, have been isolated from A. hydrophila and A. salmonicida [134]. …”

Section: Exotoxins and Other Extracellular Enzymesmentioning

confidence: 99%

See 1 more Smart Citation

The MainAeromonasPathogenic Factors

2012

View full text Add to dashboard Cite

The members of the Aeromonas genus are ubiquitous, water-borne bacteria. They have been isolated from marine waters, rivers, lakes, swamps, sediments, chlorine water, water distribution systems, drinking water and residual waters; different types of food, such as meat, fish, seafood, vegetables, and processed foods. Aeromonas strains are predominantly pathogenic to poikilothermic animals, and the mesophilic strains are emerging as important pathogens in humans, causing a variety of extraintestinal and systemic infections as well as gastrointestinal infections. The most commonly described disease caused by Aeromonas is the gastroenteritis; however, no adequate animal model is available to reproduce this illness caused by Aeromonas. The main pathogenic factors associated with Aeromonas are: surface polysaccharides (capsule, lipopolysaccharide, and glucan), S-layers, iron-binding systems, exotoxins and extracellular enzymes, secretion systems, fimbriae and other nonfilamentous adhesins, motility and flagella.

show abstract

Secreted enzymes of Aeromonas

Cited by 38 publications

References 0 publications

Extracellular cold-active lipase of Microbacterium luteolum isolated from Gangotri glacier, western Himalaya: Isolation, partial purification and characterization

Extracellular cold-active lipase of Microbacterium luteolum isolated from Gangotri glacier, western Himalaya: Isolation, partial purification and characterization

Virulence potential and antibiotic susceptibility pattern of motile aeromonads associated with freshwater ornamental fish culture systems: a possible threat to public health

The MainAeromonasPathogenic Factors

Contact Info

Product

Resources

About