Secondary sulfur metabolism in cellular signalling and oxidative stress responses

Chan, Kai Xun; Phua, Su Yin; Breusegem, Frank Van

doi:10.1093/jxb/erz119

Cited by 49 publications

(33 citation statements)

References 125 publications

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“…Line C had highest expression of this gene, followed by A; with B having significantly lower expression overall (Figure 6). Previous studies have shown that SLIM1 down regulates APK gene expression and GSL biosynthesis as a way of conserving sulfur for primary metabolism (Chan et al, 2019). Our data suggest that this is only the case between SLIM1a and APK3 (r = −0.597, q < 0.001; Supplementary Data File 1).…”

Section: Sulfur Metabolism Transcription Regulation and Transport Gmentioning

confidence: 47%

“…Also of note is that expression of MYB28 genes were positively associated with expression of SDI1 gene copies. Previous research has shown that the SDI1 protein binds to MYB28, inactivating expression and reducing GSL biosynthesis (Chan et al, 2019). In E. sativa the opposite appears to be true, with significant positive correlations between respective expression of two MYB28 copies and MYB29 with SDI1 copies (SDI1a and MYB29, r = 0.72; SDI1b and MYB28a, r = 0.507; SDI1c and MYB28c, r = 0.459; Supplementary Data File 1).…”

Section: Glucosinolate-related Transcription Factorsmentioning

confidence: 92%

“…As both glutathione and secondary S-containing metabolites, such as GSLs, have been associated with antioxidant responses (Chan et al, 2019) the differences observed between each of the lines in terms of both GSL concentrations and glutathionerelated gene expression, may be indicative of different adaptive metabolic strategies for dealing with oxidative stress postharvest. Lines A and C favor secondary sulfur metabolism and the FIGURE 6 | RNAseq expression data (FPKM) for genes involved with sulfate transport and redox response in three elite inbred lines of Eruca sativa (A, dark blue; B, light blue; C, green).…”

Section: Glutathione Synthesismentioning

confidence: 99%

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The Eruca sativa Genome and Transcriptome: A Targeted Analysis of Sulfur Metabolism and Glucosinolate Biosynthesis Pre and Postharvest

et al. 2020

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Rocket (Eruca sativa) is a source of health-related metabolites called glucosinolates (GSLs) and isothiocyanates (ITCs) but little is known of the genetic and transcriptomic mechanisms responsible for regulating pre and postharvest accumulations. We present the first de novo reference genome assembly and annotation, with ontogenic and postharvest transcriptome data relating to sulfur assimilation, transport, and utilization. Diverse gene expression patterns related to sulfur metabolism, GSL biosynthesis, and glutathione biosynthesis are present between inbred lines of rocket. A clear pattern of differential expression determines GSL abundance and the formation of hydrolysis products. One breeding line sustained GSL accumulation and hydrolysis product formation throughout storage. Multiple copies of MYB28, SLIM1, SDI1, and ESM1 have increased and differential expression postharvest, and are associated with GSLs and hydrolysis product formation. Two glucosinolate transporter gene (GTR2) copies were found to be associated with increased GSL accumulations in leaves. Monosaccharides (which are essential for primary metabolism and GSL biosynthesis, and contribute to the taste of rocket) were also quantified in leaves, with glucose concentrations significantly correlated with the expression of numerous GSL-related genes. Significant negative correlations were observed between the expression of glutathione synthetase (GSH) genes and those involved in GSL metabolism. Breeding line "B" showed increased GSH gene expression and low GSL content compared to two other lines where the opposite was observed. Co-expression analysis revealed senescence (SEN1) and oxidative stress-related (OXS3) genes have higher expression in line B, suggesting that postharvest deterioration is associated with low GSL concentrations.

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Section: Sulfur Metabolism Transcription Regulation and Transport Gmentioning

confidence: 47%

Section: Glucosinolate-related Transcription Factorsmentioning

confidence: 92%

Section: Glutathione Synthesismentioning

confidence: 99%

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The Eruca sativa Genome and Transcriptome: A Targeted Analysis of Sulfur Metabolism and Glucosinolate Biosynthesis Pre and Postharvest

et al. 2020

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“…SAM is also an important precursor for the synthesis of a group of essential metabolites including ethylene, vitamin B1, and polyamines (see Figure 1 in main text) [74,75]. PAPS has critical roles in cellular signaling and oxidative stress responses in providing activated sulfate for the sulfation of various metabolites in the secondary S metabolism including GSLs, hormones, growth-regulating peptides, and xenobiotic metabolism by sulfotransferases (SOTs) [9,76,77]. relative requirement being mirrored in the S concentration of their seeds [6].…”

Section: How Plants Cope With S Deficiencymentioning

confidence: 99%

Coordinating Sulfur Pools under Sulfate Deprivation

Aarabi

Naake

Fernie

et al. 2020

Trends in Plant Science

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Plants display manifold metabolic changes on sulfate deficiency (S deficiency) with all sulfur-containing pools of primary and secondary metabolism affected. O-Acetylserine (OAS), whose levels are rapidly altered on S deficiency, is correlated tightly with novel regulators of plant sulfur metabolism that have key roles in balancing plant sulfur pools, including the Sulfur Deficiency Induced genes (SDI1 and SDI2), More Sulfur Accumulation1 (MSA1), and GGCT2;1. Despite the importance of OAS in the coordination of S pools under stress, mechanisms of OAS perception and signaling have remained elusive. Here, we put particular focus on the general OAS-responsive genes but also elaborate on the specific roles of SDI1 and SDI2 genes, which downregulate the glucosinolate (GSL) pool size. We also highlight the key open questions in sulfur partitioning. Sulfur is a Central Component of Plant MetabolismPlants need a balanced proportion of essential macro-and micronutrients for optimal metabolic and life cycle performance. Sulfur, like the other 12 essential elements, is extracted from the soil. Photosynthetic organisms use sulfate (SO 4 2− Highlights Sulfur depletion has detrimental effects on plant metabolism and growth especially in high-S-demanding crops such as rapeseed.Plants possess multiple mechanisms of adaptation that aid the homeostasis of sulfur metabolism to allow their survival. Sulfur-deficiency-marker genes have been long identified using systems biology approaches; some of them display critical roles in balancing the sulfur pools on sulfur depletion.

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“…Sulfur plays an important role in metabolism, for example the sulfur-containing amino acids methionine is required for protein biosynthesis and cysteine is an indispensable precursor of glutathione, which is crucial to cellular redox tuning [35]. Sulfur shortage caused apparent raise in ROS level (Fig.…”

Section: The Physiological Acclimation Of C Zofingiensis To Sulfur-smentioning

confidence: 99%

Time‑resolved transcriptome analysis during transitions of sulfur nutritional status provides insight into triacylglycerol (TAG) and astaxanthin accumulation in the green alga Chromochloris zofingiensis

Mao

Lao

Sun

et al. 2020

Biotechnol Biofuels

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Background: Chromochloris zofingiensis, an oleaginous microalga, is a promising feedstock for the co-production of triacylglycerol (TAG)-based biodiesel and the high-value product astaxanthin. To reveal the molecular mechanism of TAG and astaxanthin biosynthesis during transitions of sulfur nutritional status, namely sulfur-starvation (SS) and sulfur-replenishment (SR), the physiological responses and the transcriptomic dynamics of C. zofingiensis were examined. Results: The results revealed a reversible TAG and astaxanthin accumulation under SS, which is correlated with the reduction of cell growth and protein content, indicating the reallocation of carbon. By correlating the data on the physiological and transcriptional responses to different sulfur nutritional status, a model for the underlying mechanism of TAG and astaxanthin accumulation in C. zofingiensis was postulated, which involved up-regulation of key genes including diacylglycerol acyltransferase (DGTT5) and beta-carotene ketolase (BKT1), increased energy and NADPH supply by elevating the tricarboxylic acid (TCA) cycle and the oxidative pentose phosphate (OPP) pathway, and the increased carbon precursors (pyruvate and acetyl-CoA) through central carbon metabolism. In addition, the net enhancement of the de novo biosynthesis of fatty acids and the redirection of the terpenoid precursors toward the branch catalyzed by lycopene beta cyclase (LCYb) and BKT1 escalated the substrate availability for the biosynthesis of TAG and astaxanthin, respectively. Conclusions: In this study, the time-resolved transcriptional analysis of C. zofingiensis under SS and SR conditions was reported for the first time to elucidate the regulatory roles of key enzymes, including DGTT5, BKT1 and LCYb, in the underlying mechanisms of TAG and astaxanthin accumulation.

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Secondary sulfur metabolism in cellular signalling and oxidative stress responses

Abstract: Secondary sulfur metabolism produces several metabolites which regulate various aspects of cellular signalling and homeostasis in response to environmental perturbations.

Cited by 49 publications

References 125 publications

The Eruca sativa Genome and Transcriptome: A Targeted Analysis of Sulfur Metabolism and Glucosinolate Biosynthesis Pre and Postharvest

The Eruca sativa Genome and Transcriptome: A Targeted Analysis of Sulfur Metabolism and Glucosinolate Biosynthesis Pre and Postharvest

Coordinating Sulfur Pools under Sulfate Deprivation

Time‑resolved transcriptome analysis during transitions of sulfur nutritional status provides insight into triacylglycerol (TAG) and astaxanthin accumulation in the green alga Chromochloris zofingiensis

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