Secondary Structure of Polypeptide Multilayer Films:  An Example of Locally Ordered Polyelectrolyte Multilayers

Boulmedais, Fouzia; Schwinté, Pascale; Gergely, Csilla; Voegel, J.‐C.; Schaaf, Pierre

doi:10.1021/la0117505

Cited by 80 publications

(138 citation statements)

References 21 publications

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“…14 Given the extensive interpenetration of the polymers, the final structure is not a true 2-D structure but is rather more similar to the "scrambled egg" structure of bulk polyelectrolyte complexes. 14,22,23 The bulk complex is motionally restricted due to the strong complexation between these polyions. This is expected since both PDADMAC and PSS are strong polyelectrolytes and are therefore charged completely along the length of the polymer chain.…”

Section: Resultsmentioning

confidence: 99%

¹³C Solid-State NMR Study of Polyelectrolyte Multilayers

2003

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Polyelectrolyte multilayers have been prepared by consecutively adsorbing poly(diallyldimethylammonium chloride) (PDADMAC) and poly(sodium 4-styrenesulfonate) (PSS) from aqueous solution onto colloidal silica (70-100 nm in diameter), with a total of five bilayers being prepared. The multilayer growth was followed by electrophoretic mobility as well as solid-state NMR spectroscopy. The electophoretic mobility measurements show the expected reversal in the -potential with the alternate adsorption of the polycation and polyanion. The alternation in the relative intensities observed in the 13 C solid-state NMR spectra was used to qualitatively follow the layer-by-layer growth. Relaxation measurements show that the mobility of the polyanion is found to remain constant throughout the layer-by-layer assembly, while the mobility of polycation decreases with increasing numbers of layers.

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Section: Resultsmentioning

confidence: 99%

¹³C Solid-State NMR Study of Polyelectrolyte Multilayers

2003

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“…[29] The amide I bands arising from both PGA and PLL (in D 2 O, see Experimental) appear in the 1600±1700 cm ±1 region [29,30] for the PLL/ PGA complexes in solution and for the (PLL/PGA) 6 multilayer films deposited on a solid substrate. [31] The FTIR-ATR spectrum begins to evolve as soon as the film is brought into contact with the EDC/NHS solution. The crosslinking kinetics become clear by following the difference between the spectra recorded before and after contact with EDC/NHS.…”

Section: Crosslinking Followed By Fourier-transform Ir (Ftir) Spectromentioning

confidence: 99%

Primary Cell Adhesion on RGD‐Functionalized and Covalently Crosslinked Thin Polyelectrolyte Multilayer Films

Picart¹,

Elkaïm²,

Richert

et al. 2005

Adv Funct Materials

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169

172

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Polyelectrolyte multilayers (PEMs) are now widely used for biomedical applications. In this work, we investigated the primary osteoblast adhesion properties of PEMs of poly(L‐lysine) (PLL), poly(L‐glutamic acid) (PGA), poly(alginic acid) (Palg), and poly(galacturonic acid) (Pgal). In order to compensate for the poor adhesion of the as‐synthesized films, two kinds of film modifications were achieved: a purely physical modification by film crosslinking, and a chemical modification by grafting a arginine–glycine–aspartic acid (RGD) peptide to PGA. Crosslinking was performed using a water‐soluble carbodiimide in combination with N‐hydroxysulfosuccinimide (sulfo‐NHS) to induce amide formation. This reaction was followed by Fourier‐transform IR spectroscopy. For film functionalization, a 15‐amino‐acid peptide was grafted to PGA and deposited as the top layer of the film. PLL/PGA, PLL/Palg, and PLL/Pgal films were crosslinked or functionalized. The films were tested for both short‐term adhesion properties and long‐term proliferation of primary osteoblasts. Whereas the effect of film crosslinking on short‐term adhesion was moderate, it was much more important for the RGD‐functionalized films. On the other hand, the long‐term proliferation was the same or even higher for the crosslinked films as compared with the functionalized films. This effect was particularly enhanced for the PLL/Palg and PLL/Pgal films. Finally, we functionalized PLL/PGA that had been crosslinked prior to PGA‐RGD deposition. These architectures exhibited even higher short‐term adhesion and proliferation. These results clearly show the important role of the physical properties of the films, besides their chemical properties, for the modulation of primary cell‐adhesion behavior.

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“…Polyelectrolyte multilayers can be considered as "layered complexes" held together by the same interactions as soluble complexes, [5,[62][63][64][65] but exhibiting a higher segment density. [66] Owing to their high hydration and their swelling properties, they can be viewed as dense hydrogels, [67] in which the crosslinking density is controlled by the charge distribution along the chain.…”

Section: Mechanism Of the Dissolution Processmentioning

confidence: 99%

Controlled Electrodissolution of Polyelectrolyte Multilayers: A Platform Technology Towards the Surface‐Initiated Delivery of Drugs

Boulmedais

Tang

Keller

et al. 2005

Adv Funct Materials

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113

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A novel method for the electrochemical dissolution of polyelectrolyte multilayers from the surface of an electrode for applications in controlled drug delivery is reported. Biodegradable and biocompatible multilayer films based on poly(L‐lysine) and heparin have been selected as a model system, and have been built on an indium tin oxide semiconductor substrate. The build‐up and dissolution processes of the multilayers is followed by electrochemical optical waveguide light mode spectroscopy. The formation and stability of the polyelectrolyte multilayers have been found to depend on the applied potential and the ionic strength of the buffer. The application of potentials above a threshold of 1.8 V induces dissolution, which follows single‐exponential kinetics, of the polyelectrolyte multilayer film. The rate of this process can be varied by an on–off profile of the potential, leading to the controlled release of heparin into the bulk. Atomic force microscopy investigations show that the electrodissolution of the polyelectrolyte multilayers is a local phenomenon that leads to the formation of nanoporous films.

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Secondary Structure of Polypeptide Multilayer Films: An Example of Locally Ordered Polyelectrolyte Multilayers

Cited by 80 publications

References 21 publications

¹³C Solid-State NMR Study of Polyelectrolyte Multilayers

¹³C Solid-State NMR Study of Polyelectrolyte Multilayers

Primary Cell Adhesion on RGD‐Functionalized and Covalently Crosslinked Thin Polyelectrolyte Multilayer Films

Controlled Electrodissolution of Polyelectrolyte Multilayers: A Platform Technology Towards the Surface‐Initiated Delivery of Drugs

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Secondary Structure of Polypeptide Multilayer Films: An Example of Locally Ordered Polyelectrolyte Multilayers

Cited by 80 publications

References 21 publications

13C Solid-State NMR Study of Polyelectrolyte Multilayers

13C Solid-State NMR Study of Polyelectrolyte Multilayers

Primary Cell Adhesion on RGD‐Functionalized and Covalently Crosslinked Thin Polyelectrolyte Multilayer Films

Controlled Electrodissolution of Polyelectrolyte Multilayers: A Platform Technology Towards the Surface‐Initiated Delivery of Drugs

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¹³C Solid-State NMR Study of Polyelectrolyte Multilayers

¹³C Solid-State NMR Study of Polyelectrolyte Multilayers