Secondary somatic embryogenesis versus caulogenesis from somatic embryos of Aesculus carnea Hayne.: developmental stage impact

Zdravković-Korać, Snežana; Ćalić﻿﻿﻿, Dušica; Uzelac, Branka; Janošević, Dušica; Budimir, Snežana; Vinterhalter, Branka; Vinterhalter, D.

doi:10.1007/s11240-008-9399-4

Cited by 8 publications

(17 citation statements)

References 19 publications

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“…Our results show that a greater number of secondary somatic embryos are produced in an earlier phase of development with a lower number of embryos. These results are in agreement with the results obtained for red chestnut (Zdravković-Korać et al, 2008).…”

Section: Discussionsupporting

confidence: 93%

Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis

Ćalić¹,

Devrnja²,

Milojević³

et al. 2012

horts

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The effect of abscisic acid on the development of primary androgenic embryo and secondary somatic embryogenesis was investigated with the aim of improving multiplication rates and secondary somatic embryo quality in horse chestnut microspore and anther culture. The early embryo stage (globular) had a better response than late stages (heart, torpedo, and cotyledonary) in both types of cultures. Also, microspore culture had a high potential for mass secondary embryo production. The number of secondary somatic embryos was three times higher on hormone-free medium than on medium enriched with 0.01 mg·L−1 abscisic acid. However, most of the embryos on hormone-free medium had abnormal morphology. For this reason, abscisic acid was added to the media to improve embryo quality. The morphology of abscisic acid treated embryos was better than abscisic acid non-treated embryos. The optimal abscisic acid concentration for secondary somatic embryo induction and production of high-quality embryos was 0.01 mg·L−1. Overall, the effect of abscisic acid on the induction of secondary somatic embryogenesis and plant regeneration of androgenic embryos of this species may be helpful for the further synthesis of secondary metabolites in vitro and their application in the pharmaceutical industry.

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Section: Discussionsupporting

confidence: 93%

Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis

Ćalić¹,

Devrnja²,

Milojević³

et al. 2012

horts

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“…The SEC indexes in G embryos were higher than the indexes reported by Á lvarez et al (2009) in P. pinaster germinated cotyledons; the reason for better values in our experiments could be the abovementioned higher organogenic response in G explants when compared with NG explants. Similar findings have been found in some angiosperms species such as red horse chestnut, Aesculus carnea (Zdravković-Korać et al 2008).…”

Section: Discussionsupporting

confidence: 89%

“…(Khan et al 2006), Aesculus carnea (Zdravković-Korać et al 2008) and Hippophae rhamnoides (Sriskandarajah and Lundquist 2009). The goal of these studies was to study the potential of both techniques, through media and growth regulator adjustments, in the regeneration of these species.…”

Section: Discussionmentioning

confidence: 99%

A combined pathway of somatic embryogenesis and organogenesis to regenerate radiata pine plants

Montalbán

Diego

Igartua

et al. 2011

Plant Biotechnol Rep

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This study describes for the first time in Pinus genus a plant regeneration system via a combined pathway of somatic embryogenesis and organogenesis from immature seeds of radiata pine. Somatic embryos were obtained from embryogenic line 2162 of Pinus radiata D. Don on EDM basal medium containing 60 lM ABA and 6% sucrose. The explants used for organogenesis experiments were either freshly collected somatic embryos or somatic embryos germinated for 1 week. Germination medium was half-strength LP medium, supplemented with 0.2% activated charcoal. Different induction periods and BA concentrations were assayed for shoot induction. After induction treatments, explants were elongated on the same medium used for germination stage. Rooting medium was quarter-strength LP medium supplemented with three different auxin treatments: 1.5 mg L -1 1-naphthalene acetic acid (NAA), 1.5 mg L -1 indole-3-butyric acid (IBA) and 1 mg L -1 IBA with 0.5 mg L -1 NAA (MIX). The effect of the photon flux (120 mmol m -2 s -1 and darkness) in the first week of the explants in the rooting media was also tested. This methodology could offer an alternative to overcome some problems associated with somatic embryogenesis such as the seasonality of embryogenic tissue (ET) initiation or a low embryo production from the ET, a particularly important issue in the case of genetically transformed ETs.

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“…AEs and SEs of Aesculus sp. spontaneously regenerated SSEs on medium devoid of PGRs [86,87,109,110], but the frequency of secondary somatic embryogenesis could be rather low, as was observed in A. carnea (3-39%) [87]. Cytokinins (1-10 µM BA or Kin) significantly amplified this process, increasing PSE response to 81% in A. carnea [87].…”

Section: Secondary Somatic Embryogenesismentioning

confidence: 98%

“…Aesculus species are prone to somatic embryogenesis, thus it is not surprising that somatic embryogenesis was successfully achieved with almost all horse chestnut plant organs used as initial explants: stamen filaments [76][77][78], immature zygotic embryos [77,79], seedling's primary leaves [80] and cotyledons [81], and stem [82] and bark fragments [83] isolated from young terminal branches of adult trees. Somatic embryogenesis was also initiated from stems [84] and shoots [85] of A. glabra, stamen filaments of A. flava [86] and A. carnea [87], shoots and roots of 4-week-old seedlings and shoots of a 30-year-old tree of a hybrid A. x arnoldiana [85]. In all the studies, somatic embryogenesis was induced indirectly through a callus phase, with the exception of stamen filaments of A. hippocastanum, in which SEs regenerated directly from filaments in 10% of the explants, while in the remaining 90% of the explants SEs regenerated indirectly from embryogenic callus [77].…”

Section: Somatic Embryogenesismentioning

confidence: 99%

Tissue Culture Response of Ornamental and Medicinal Aesculus Species—A Review

Zdravković-Korać

Milojević

Belić

et al. 2022

Plants

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Species of the genus Aesculus are very attractive woody ornamentals. Their organs contain numerous health‑promoting phytochemicals. The most valuable of them—aescin—is used in commercial preparations for the treatment of venous insufficiency. The industrial source of aescin is horse chestnut seeds because the zygotic embryos are the main site of its accumulation. Horse chestnut somatic and zygotic embryos contain similar amount of aescin, hence somatic embryos could be exploited as an alternative source of aescin. Somatic embryogenesis, androgenesis and de novo shoot organogenesis were successfully achieved in several Aesculus species, as well as secondary somatic embryogenesis and shoot organogenesis, which enables mass production of embryos and shoots. In addition, an efficient method for cryopreservation of embryogenic tissue was established, assuring constant availability of the plant material. The developed methods are suitable for clonal propagation of elite specimens selected as the best aescin producers, the most attractive ornamentals or plants resistant to pests and diseases. These methods are also useful for molecular breeding purposes. Thus, in this review, the medicinal uses and a comprehensive survey of in vitro propagation methods established for Aesculus species, as well as the feasibility of in vitro production of aescin, are presented and discussed.

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Secondary somatic embryogenesis versus caulogenesis from somatic embryos of Aesculus carnea Hayne.: developmental stage impact

Cited by 8 publications

References 19 publications

Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis

Abscisic Acid Effect on Improving Horse Chestnut Secondary Somatic Embryogenesis

A combined pathway of somatic embryogenesis and organogenesis to regenerate radiata pine plants

Tissue Culture Response of Ornamental and Medicinal Aesculus Species—A Review

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