Secondary Metabolome and Transcriptome of Streptomyces albus J1074 in Liquid Medium SG2

Koshla, Oksana; Rokytskyy, Ihor; Ostash, Iryna; Busche, Tobias; Kalinowski, Jörn; Mösker, Eva; Fedorenko, Victor; Ostash, Bohdan

doi:10.3103/s0095452719010080

Cited by 5 publications

(5 citation statements)

References 18 publications

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“…Although apparently widespread in marine-derived Streptomyces isolates [18,19], the production of surugamides has also been reported in the S. albidoflavus strain J1074 [16,22], a derivative of the soil isolate S. albus G [23,24]. The S. albidoflavus strain J1074 is a well-characterised Streptomyces isolate, which is frequently used as a model for the genus and has commonly been successfully employed in the heterologous expression of biosynthetic gene clusters (BGCs) [25,26,27,28,29].…”

Section: Introductionmentioning

confidence: 99%

“…This strain was originally classified as an S. albus isolate, however, due to more recent taxonomy studies, it has been reclassified as a S. albidoflavus species isolate [30,31]. Interestingly, surugamides and their derivatives have been shown to only be produced by S. albidoflavus J1074 under specific conditions, such as when employing chemical stress elicitors [16], and more recently when cultivating the strain in a soytone-based liquid-based medium SG2 [22].…”

Section: Introductionmentioning

confidence: 99%

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Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge Isolate Streptomyces sp. SM17 When Compared to Its Terrestrial Relative S. albidoflavus J1074

et al. 2019

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Much recent interest has arisen in investigating Streptomyces isolates derived from the marine environment in the search for new bioactive compounds, particularly those found in association with marine invertebrates, such as sponges. Among these new compounds recently identified from marine Streptomyces isolates are the octapeptidic surugamides, which have been shown to possess anticancer and antifungal activities. By employing genome mining followed by an one strain many compounds (OSMAC)-based approach, we have identified the previously unreported capability of a marine sponge-derived isolate, namely Streptomyces sp. SM17, to produce surugamide A. Phylogenomics analyses provided novel insights on the distribution and conservation of the surugamides biosynthetic gene cluster (sur BGC) and suggested a closer relatedness between marine-derived sur BGCs than their terrestrially derived counterparts. Subsequent analysis showed differential production of surugamide A when comparing the closely related marine and terrestrial isolates, namely Streptomyces sp. SM17 and Streptomyces albidoflavus J1074. SM17 produced higher levels of surugamide A than S. albidoflavus J1074 under all conditions tested, and in particular producing >13-fold higher levels when grown in YD and 3-fold higher levels in SYP-NaCl medium. In addition, surugamide A production was repressed in TSB and YD medium, suggesting that carbon catabolite repression (CCR) may influence the production of surugamides in these strains.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge Isolate Streptomyces sp. SM17 When Compared to Its Terrestrial Relative S. albidoflavus J1074

et al. 2019

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“…In this work, the KO-1297L strain was obtained as a spontaneous streptomycin-sensitive (Str S ) KO-1297 derivative. Streptomycetes were cultivated on TSA, GYM, SG2, TSB, and SFM media (Tanaka et al, 2009;Koshla et al, 2017;Koshla et al, 2019) at 30°C. The strains Bacillus cereus ATCC19637, Staphylococcus aureus 209P, and Debaryomyces hansenii VKM9 were used as test culture for determining the antibiotic activity of rpsL mutants.…”

Section: Methodsmentioning

confidence: 99%

Properties of Spontaneous rpsL Mutant of Streptomyces albus KO-1297

et al. 2022

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The Streptomyces albus J1074 strain remains one of the most popular platforms for the discovery of new natural compounds due to the expression of biosynthetic gene clusters (BGCs) from the microorganisms of the Actinobacteria class. Different methods were tested to provide a maximal expression of heterologous BGCs in this strain. However, there is still no description of the properties of spontaneous J1074 mutants in the rpsL gene encoding a ribosomal protein S12. The interest in such mutations in actinobacteria is due to the fact that they provide a considerable increase in the antibiotic activity. In this work, we describe the isolation and characterization of the S. albus KO-1297 strain, which contains a spontaneous missense mutation in the rpsL gene leading to a Lys88Glu substitution in the protein S12. As compared with the initial strain, this mutant exhibits an increased resistance to streptomycin and higher antibiotic productivity. The KO-1297 strain and genetically engineered rpsL K88E mutant K88E are not identical in their ability to produce antibiotics. KO-1297 also exhibits a certain level of instability of rpsL mutation. The genomes of KO-1297 and its rpsL WT revertant contain the mutations that can cause phenotypic differences between these strains (as well as between them and SAM2 and K88E strains).

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“…The known antifungal compounds produced endogenously consist of candicidins, antimycins, alteramides and surugamides [ 17 ]. Recently, it was also revealed that certain media such as SG2 was able to enhance production of these secondary metabolites [ 18 ], which was attributable to the overall increased transcriptional activity evidenced by RNA-seq studies on the organism grown in both SG2 and the more conventional TSB media. Additionally, novel strains of S. albus have been constructed with their native biosynthetic gene clusters removed [ 10 ], consequently leading not only to better compound detection (due to no background from endogenous secondary metabolites) but also to overall higher production yields compared to the ancestor S. albus strain and other S. coelicolor hosts.…”

Section: Introductionmentioning

confidence: 99%

Reconstruction of a Genome-Scale Metabolic Model of Streptomyces albus J1074: Improved Engineering Strategies in Natural Product Synthesis

Kittikunapong

Magadán‐Corpas

et al. 2021

Metabolites

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Streptomyces albus J1074 is recognized as an effective host for heterologous production of natural products. Its fast growth and efficient genetic toolbox due to a naturally minimized genome have contributed towards its advantage in expressing biosynthetic pathways for a diverse repertoire of products such as antibiotics and flavonoids. In order to develop precise model-driven engineering strategies for de novo production of natural products, a genome-scale metabolic model (GEM) was reconstructed for the microorganism based on protein homology to model species Streptomyces coelicolor while drawing annotated data from databases and literature for further curation. To demonstrate its capabilities, the Salb-GEM was used to predict overexpression targets for desirable compounds using flux scanning with enforced objective function (FSEOF). Salb-GEM was also utilized to investigate the effect of a minimized genome on metabolic gene essentialities in comparison to another Streptomyces species, S. coelicolor.

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Secondary Metabolome and Transcriptome of Streptomyces albus J1074 in Liquid Medium SG2

Cited by 5 publications

References 18 publications

Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge Isolate Streptomyces sp. SM17 When Compared to Its Terrestrial Relative S. albidoflavus J1074

Genome Mining Coupled with OSMAC-Based Cultivation Reveal Differential Production of Surugamide A by the Marine Sponge Isolate Streptomyces sp. SM17 When Compared to Its Terrestrial Relative S. albidoflavus J1074

Properties of Spontaneous rpsL Mutant of Streptomyces albus KO-1297

Reconstruction of a Genome-Scale Metabolic Model of Streptomyces albus J1074: Improved Engineering Strategies in Natural Product Synthesis

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