Secondary Metabolites in in vitro Cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams

Enriz, Ricardo Daniel; Freile, M.L.; Correche, María Susana; Gómez-Lechón, Marı́a José

doi:10.1201/b10756-17

Cited by 17 publications

(28 citation statements)

References 75 publications

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“…It was also shown in our studies on lignans accumulation in S. chinensis cultures at different stages of differentiation Ekiert 2011, 2013) and in Ruta graveolens and Ruta graveolens ssp. divaricata cultures, which accumulated different amounts of linear furanocoumarins depending on the degree of organogenesis (Ekiert and Czygan 2007). Likewise, shoots from R. graveolens in vitro cultures (Ekiert et al 2009) and shootdifferentiating callus cultures from Ruta graveolens ssp.…”

Section: Discussionmentioning

confidence: 99%

“…chlorogenic and rosmarinic acids, are a therapeutically important group of plant metabolites. These compounds possess long-known anti-inflammatory, cholagogic, spasmolytic, hypolipemic and antiaggregatory properties (De Graff et al 2003;Ekiert and Czygan 2007;Nakamura et al 2001;Sroka and Cisowski 2003). More recent studies have also revealed significant antioxidant, anti-free radical, immunostimulating and anticancer activities of some representatives of this group, e.g.…”

Section: Introductionmentioning

confidence: 99%

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Production of biologically active phenolic acids in Aronia melanocarpa (Michx.) Elliott in vitro cultures cultivated on different variants of the Murashige and Skoog medium

Szopa

2013

Plant Growth Regul

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Phenolic acids, both benzoic and cinnamic acid derivatives, are plant metabolites with high therapeutic and cosmetic values. Methanolic extracts from the biomass of shoot and callus cultures of Aronia melanocarpa growing on seven variants of the Murashige and Skoog (MS) medium with different concentrations of plant growth regulators, BA and NAA, ranging from 0.1 to 3.0 mg l -1 , were examined for the production of free phenolic acids and cinnamic acid using the high-performance liquid chromatography (HPLC) method. The extracts from the shoot and callus cultures were confirmed to contain five of the twelve compounds tested for: caffeic, p-coumaric, p-hydroxybenzoic, syringic and vanillic acids. The shoot extracts contained additionally salicylic acid. Both the total amounts and the amounts of individual compounds in either the shoot or callus extracts were dependent on the concentration of cytokinin and auxin in the MS medium variants. The total amounts in the shoot and callus cultures were in the range from 93.52 to 217.00 mg 100 g -1 DW and from 47.11 to 83.83 mg 100 g -1 DW, respectively. The amounts of individual compounds showed wide variation, from 1.31 to 91.86 mg 100 g -1 DW in the shoot extracts, and from 2.58 to 40.16 mg 100 g -1 DW in the callus extracts. Salicylic acid (max. 91.86 mg 100 g -1 DW), p-coumaric acid (max. 62.39 mg 100 g -1 DW) and p-hydroxybenzoic acid (max. 50.66 mg 100 g -1 DW) dominated in the shoot extracts, while syringic acid (max. 40.16 mg 100 g -1 DW) and p-hydroxybenzoic acid (max. 23.59 mg 100 g -1 DW) were the main metabolites in the callus extracts. This is the first report on the quantitative analysis of benzoic and cinnamic acid derivatives in shoot and callus cultures of A. melanocarpa growing on MS-based media with different concentrations of selected plant growth regulators-BA and NAA. The obtained maximum amounts of some metabolites are of interest from a practical perspective.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Production of biologically active phenolic acids in Aronia melanocarpa (Michx.) Elliott in vitro cultures cultivated on different variants of the Murashige and Skoog medium

Szopa

2013

Plant Growth Regul

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“…Many recent studies have also confirmed strong antioxidant, antiradical and anticancer activities of some compounds of this group, e.g. caffeic acid, p-coumaric and protocatechuic acids (Laranjinha et al 1994;Nardini et al 1995;Rice-Evans et al 1996;Sroka and Cisowski 2003;Wen et al 2003;Ekiert and Czygan 2007;Sánchez-Maldonado et al 2011). Hydroxybenzoic acids also possess many others very important biological properties.…”

Section: Introductionmentioning

confidence: 98%

Accumulation of hydroxybenzoic acids and other biologically active phenolic acids in shoot and callus cultures of Aronia melanocarpa (Michx.) Elliott (black chokeberry)

Szopa

Muszyńska

2012

Plant Cell Tiss Organ Cult

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Phenolic acids are plant metabolites important in phytotherapy and also in cosmetology. In this study, proliferating shoot and callus cultures of Aronia melanocarpa were established and maintained on Linsmaier and Skoog (L-S) medium containing different levels of a-naphthaleneacetic acid (NAA) and 6-benzyladenine (BA), ranging from 0.1 to 3.0 mg l -1 . Methanolic extracts from the biomass of these cultures and from the fruits of soil-grown plants were used to determine the amounts of free phenolic acids and cinnamic acid using the high-performance liquid chromatography (HPLC) method. Out of a total of twelve analyzed compounds, all of the extracts contained four of them: caffeic acid, p-hydroxybenzoic acid, syringic acid, and vanillic acid. Moreover, shoot extracts also contained salicylic acid (o-hydroxybenzoic acid), while callus extracts contained p-coumaric acid. On the other hand, fruit extracts also contained both salicylic acid and p-coumaric acid. The total amount of the analyzed compounds in extracts from both shoot and callus cultures depended on the L-S medium used, and varied between 103.05 and 150.95 mg 100 g -1 dry weight (DW), and between 50.23 and 81.56 mg 100 g -1 DW, respectively. Both types of culture contained higher levels of phenolic acids than the fruit extracts (32.43 mg 100 g -1 DW). In shoot cultures, p-hydroxybenzoic acid and salicylic acid were the predominant metabolites (reaching 55.14 and 78.25 mg 100 g -1 DW, respectively), while in callus cultures, p-hydroxybenzoic acid (25.60 mg 100 g -1 DW) and syringic acid (41.20 mg 100 g -1 DW) were the main compounds. In fruit extracts, salicylic acid (15.60 mg 100 g -1 DW) and p-hydroxybenzoic acid (5.29 mg 100 g -1 DW) were predominant.

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“…Our preliminary studies indicated a marked effect of monochromatic light on the accumulation of both phenolic acids and linear furanocoumarins in in vitro cultures of R. graveolens and its subspecies (Ekiert and Czygan 2007;Ekiert and Gomółka 1999).…”

Section: Introductionmentioning

confidence: 99%

“…These compounds exhibit numerous biological activities, the most important of which are their immunostimulating, antioxidant and anticancer properties (Abdel-Wahab et al 2003;De Graff et al 2003;Ekiert and Czygan 2007;Nakamura et al 2001;Sroka and Cisowski 2003). Linear furanocoumarins have an established position in therapy as antiproliferative and photosensitizing agents, and some of them are calcium and potassium channel blockers (Bohuslavizki et al 1994;Ekiert and Czygan 2007).…”

Section: Introductionmentioning

confidence: 99%

Production of bioactive phenolic acids and furanocoumarins in in vitro cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams. under different light conditions

Szopa

Ekiert

Szewczyk

et al. 2012

Plant Cell Tiss Organ Cult

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Secondary Metabolites in in vitro Cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams

Cited by 17 publications

References 75 publications

Production of biologically active phenolic acids in Aronia melanocarpa (Michx.) Elliott in vitro cultures cultivated on different variants of the Murashige and Skoog medium

Production of biologically active phenolic acids in Aronia melanocarpa (Michx.) Elliott in vitro cultures cultivated on different variants of the Murashige and Skoog medium

Accumulation of hydroxybenzoic acids and other biologically active phenolic acids in shoot and callus cultures of Aronia melanocarpa (Michx.) Elliott (black chokeberry)

Production of bioactive phenolic acids and furanocoumarins in in vitro cultures of Ruta graveolens L. and Ruta graveolens ssp. divaricata (Tenore) Gams. under different light conditions

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