Secondary metabolites from Triclisia gilletii (De Wild) Staner (Menispermaceae) with antimycobacterial activity against Mycobacterium tuberculosis

Abstract

Triclisinone (2), a new ochnaflavone derivative, was isolated from the aerial parts of Triclisia gilletii, along with known drypemolundein B (1) and eight other known compounds. The chemical shifts of drypemolundein B (1) have been partially revised based on reinterpretation of NMR spectroscopic data. The eight other secondary metabolites are composed of: (+)-nonacosan-10-ol (3); stigmasterol (4), 3-O-β-D-glucopyranosylsitosterol (5), 3-O-β-D-glucopyranosylstigmasterol (6); oleanic acid (7); myricetin (8), quercetin (9) and 3-methoxyquercetin (10). Their structures were elucidated using IR, MS, NMR 1D and 2D, H andC and comparison with literature data. Furthermore, compounds 1, 2, 5, 6, 8, 9 and the crude extract were tested against Mycobacterium tuberculosis. Compounds 1, 2, 8 and 9 displayed moderate to very good activity against resistant strain (codified AC 45) of M. tuberculosis with minimum inhibitory concentrations MICs ranging from 3.90 to 62.5 μg/mL.

Effect of moonseed vine (Triclisia gilletii Staner) on ethane-1,2-diol-induced urolithiasis and its renotoxicity in Wistar albino rats

“…In Ondo State, located in the South Western part of Nigeria, the plant which is usually called 'Peshe' in Ondo City or 'Ogbogan' in Okeluse, Ondo State, Nigeria, and Akan-Akyem Sanhoma in Ghana is used locally for the management of swelling of extremities and other kidney-related diseases or infections either singly or in combination with other condiments by traditional practitioners. Tiam et al [12] reported its usage for the management of edema and anemia which by our bet is closest to kidney function.…”

“…Increased proteinuria, albuminuria, and glucosuria in this study indicate possible glomerular and tubular damage as a result of ethane-1,2-diol administration. The protectivity of TGME on the renal epithelial could be ascribed to the antioxidant power of quercetin and oleanolic acid present in the plant [12].…”

“…The genus Triclisia comprises 20 species throughout the world with 12 in tropical Africa Region. Plants belonging to this genus possess potential therapeutic values known since ancient times to cure various ailments and infectious diseases such as malaria, venereal diseases, epileptic attacks, diarrhea, stomach problems, leprosy, mental health problems, dysentery, respiratory diseases, convulsive coughing, and renalrelated ailments including edema, anemia, gout, urinogenital infections, and swelling of extremities [9][10][11][12].…”

“…Various classes of bioactive compounds have been isolated and identified [12]. These include flavonoids (2′-hydroxy-4′-methoxyochnaflavone, myricetin, quercetin, and 3-methoxyquercetin), steroids (nonacosan-10-ol, stigmasterol, 3-O-β-d-glucopyranosylsitosterol, and 3-O-β-d-glucopyranosylstigmasterol), and triterpene (oleanolic acid).…”

Density Functional Theory of a New Derivative of Allantoin with Antiplasmodial Properties, Antimycobacterial Activities of Essential Oil from Cordia Batesii WERNHAM (Boraginaceae)

“…Melting points were uncorrected and were measured on a Mettler Toledo instrument. IR spectra were recorded on an Alpha FT-IR spectrometer from Bruker, while 1D and 2D NMR spectra were obtained on a Bruker DRX 500 (500 MHz for 1 H and 125 MHz for 13 Silica gel 60 (230-400 mesh E. Merck, Darmstadt, Germany) and Sephadex® LH-20 were employed for CC, the solvent mixing systems for elution were mainly CH2Cl2/MeOH for the phytochemical study with increasing polarity and pure MeOH, while precoated aluminum sheets silica gel 60 F254 were used for TLC [47].…”