Secondary metabolite production in Hypericum perforatum L. cell suspensions upon elicitation with fungal mycelia from Aspergillus flavus

Abstract

We investigated the production of phenylpropanoids (phenolic compounds, flavanols, flavonols and anthocyanins) and naphtodianthrones (hypericins) in elicited Hypericum perforatum L. cell suspensions. To determine whether secondary metabolite production could be enhanced, Hypericum cell suspensions were exposed to mycelia extract from the fungus Aspergillus flavus. Elicited Hypericum cell suspension cultures displayed reduced growth and viability and a modification of secondary metabolites production. Anthocyanins were only stimulated in fungal-elicited cell suspensions. Secondary metabolite production in elicited Hypericum cells revealed an antagonism between the flavonoid/naphtodianthrone and anthocyanin pathways. The data suggest a modification of the channeling of the phenylpropanoid compounds. Together, these results represent useful data for monitoring the channeling in different secondary metabolite pathways during the scaled-up production of naphtodianthrones for medicinal uses

Fungal elicitor-mediated enhancement in phenylpropanoid and naphtodianthrone contents of Hypericum perforatum L. cell cultures

“…Present results showed that exogenously applied fungal mycelia extracts negatively affected cell viability and fresh biomass production of H. perforatum cell cultures. Such a result has already been reported when H. perforatum cell suspensions have been elicited with JA, SA and fungal mycelia from A. flavus (Gadzovska et al 2007(Gadzovska et al , 2013Gadzovska-Simic et al 2012). On the other side, JA significantly stimulated biomass production of H. perforatum cells (Walker et al 2002), while treatments with SA, P. cinnamoni and Agrobacterium failed to show any stimulatory effect on cell growth (Walker et al 2002;Tusevski et al 2014).…”

“…Fusarium was shown as superior elicitor in stimulation of TP and TF production, while all tested fungal elicitors gave a similar response with respect to TFL and TA accumulation. Phenylpropanoid production in H. perforatum cell suspensions has also been stimulated using JA, SA, A. flavus and Agrobacterium as elicitors (Gadzovska et al 2007(Gadzovska et al , 2013; Gadzovska-Simic et al 2012;Tusevski et al 2014). The b Fig.…”

“…Present results suggested that fungal elicitors stimulated HYP and PHYP production during the entire post-elicitation period. The production of HYPs in H. perforatum cultures has also been stimulated upon treatments with fungal elicitors such as P. cinnamoni, C. gloeosporioides and A. flavus (Walker et al 2002;Sirvent and Gibson 2002;Gadzovska-Simic et al 2012). The levels of HYPs presented in H. perforatum plant tissues as a response to C. gloeosporioides invasion have been directly toxic to the fungus and caused inhibition of mycelia growth in vitro (Sirvent and Gibson 2002).…”

“…We have previously studied the overproduction of phenylpropanoids and naphtodianthrones in H. perforatum cultures upon treatments with phytohormones (Gadzovska et al 2005), signal molecules jasmonic acid (JA) and salicylic acid (SA), (Gadzovska et al 2007(Gadzovska et al , 2013, fungal elicitor Aspergillus flavus (Gadzovska-Simic et al 2012) and polysaccharides ). Various H. perforatum in vitro cultures have also been tested for their ability to produce naphtodianthrones, acylphloroglucinols, flavonoids and xanthones upon treatment with mannan, b-1,3-glucan, pectin, JA, methyl jasmonate, SA, fungal elicitors Phytophthora cinnamoni and Colletotrichum gloeosporioides, as well with bacterial elicitors Agrobacterium tumefaciens and A. rhizogenes (Kirakosyan et al 2000;Sirvent and Gibson 2002;Walker et al 2002;Tusevski et al 2014).…”

Agrobacterium enhances xanthone production in Hypericum perforatum cell suspensions

“…Several studies have been carried out to investigate the effects of different elicitors on the accumulation of secondary metabolites in Hypericum in vitro cultures. We have previously studied the overproduction of phenylpropanoids and naphtodianthrones in H. perforatum in vitro cultures upon treatment with phytohormones (Gadzovska et al 2005) and various elicitors, such as jasmonic acid (Gadzovska et al 2007), Aspergillus flavus mycelia extract (Gadzovska-Simic et al 2012) and salicylic acid ). In addition, various in vitro cultures from H. perforatum have been tested for their ability to produce naphtodianthrones and acylphloroglucinols upon treatment with mannan, b-1,3-glucan, pectin, jasmonic acid, methyl jasmonate, salicylic acid, fungal elicitor Phytophthora cinnamoni and bacterial elicitor Agrobacterium tumefaciens (Kirakosyan et al 2000;Sirvent and Gibson 2002;Walker et al 2002;Pavlík et al 2007).…”