Secondary ion mass spectrometry with cesium ion primary beam and liquid target matrix for analysis of bioorganic compounds

Aberth, W.; Straub, Kenneth; Burlingame, Alma L.

doi:10.1021/ac00249a028

Cited by 290 publications

(90 citation statements)

References 27 publications

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“…Pigments and sterols were eluted with 4 mL chloroform. Steryl glycosides and glycosylceramides were eluted with 4 mL chloroform/methanol (1:1) followed by 4 (1). For LSIMS, samples were dissolved in a glycerol matrix.…”

Section: Identification Of Glycosylceramidesmentioning

confidence: 99%

Lipid Composition of Plasma Membranes and Endomembranes Prepared from Roots of Barley (Hordeum vulgare L.)

Brown¹,

DuPont²

1989

Plant Physiol.

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ABSTRACTporters allows cells to accumulate essential ions while excluding ions which are toxic. The exclusion of Na+, for example, is believed to be an important trait of salt-tolerant barley cultivars (21). Selective transport also occurs at the tonoplast and other endomembranes. The selectivity ofeach membrane varies with the types of ion channels and pumps that are present, while the efficacy of each membrane as a barrier may vary with the type and proportion of its lipid components (4, 5, 7). The major lipid components of plant membranes are phospholipids. glycolipids, and sterols. The specific proportions of these lipids in the PM (24,29,30) and the tonoplast (25, 32) are known for a variety of plant tissues. Only one study (34) has examined the lipid composition of both the PM and tonoplast prepared from a single source.There is some evidence that salt stress can induce changes in plant membrane lipids. A survey of plant species of varying salt-tolerance reported an increase in the ratio of glycolipid to phospholipid in the roots of both a halophyte, Atriplex gmelinia, and the salt-sensitive cucumber, Cucumis sativa L., when plants were grown in increasing concentrations of NaCl (18). In Citrus roots, the sterol composition was altered in salt-stressed plants and some of these changes occurred at the PM (8, 9). It was not known ifsalt affects the lipid composition of the PM or endomembranes of barley.Membrane fractions that are enriched in PM, tonoplast, and ER are prepared by centrifuging a microsomal pellet prepared from barley roots through a sucrose step gradient (11,12). When barley plants are grown in 100 mM NaCl the distribution of marker enzymes on sucrose gradients does not change, but the protein compositions of the PM, endomembrane, and cytoplasmic fractions are altered (19,20), and a Na+/H+ exchange is activated in the tonoplast membranes. Shoot growth is reduced but the plants show no signs ofinjury (19). In this study we identify and quantify the phospholipids, glycolipids, and sterols contained in the three membrane fractions. The effect of a high but noninjurious concentration phatidylethanolamine; PI, phosphatidylinositol; PS, phosphatidylserine; TG, tonoplast and Golgi membranes; 24v-methylcholesterol, campesterol or dihydrobrassicasterol, orientation of methyl group at carbon 24 not determined.www.plantphysiol.org on May 9, 2018 -Published by Downloaded from

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Section: Identification Of Glycosylceramidesmentioning

confidence: 99%

Lipid Composition of Plasma Membranes and Endomembranes Prepared from Roots of Barley (Hordeum vulgare L.)

Brown¹,

DuPont²

1989

Plant Physiol.

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“…Finally, these early efforts required a substantial amount of mass for adequate signal-to-noise ratios and suffered from an inability to accurately quantify minor mass constituents, which are of substantial importance in lipid-mediated signaling processes. These facts notwithstanding, substantial insights were made through HPLC, fast atom bombardment/MS, and gas chromatography/MS approaches in the 1980s (7,(8)(9)(10)(11), and the stage was set for the growth of lipidomics in the 1990s.…”

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confidence: 99%

Global analyses of cellular lipidomes directly from crude extracts of biological samples by ESI mass spectrometry: a bridge to lipidomics

Han

Gross

2003

Journal of Lipid Research

816

601

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Lipidomics is a rapidly expanding research field in which multiple techniques are utilized to quantitate the hundreds of chemically distinct lipids in cells and determine the molecular mechanisms through which they facilitate cellular function. Recent developments in electrospray ionization mass spectrometry (ESI/MS) have made possible, for the first time, the precise identification and quantification of alterations in a cell's lipidome after cellular perturbations. This review provides an overview of the essential role of ESI/MS in lipidomics, presents a broad strategy applicable for the generation of lipidomes directly from cellular extracts of biological samples by ESI/MS, and summarizes salient examples of strategies utilized to conquer the lipidome in physiologic signaling as well as pathophysiologically relevant disease states. Because of its unparalleled sensitivity, specificity, and efficiency, ESI/MS has provided a critical bridge to generate highly accurate data that fingerprint cellular lipidomes to facilitate insight into the functional role of subcellular membrane compartments and microdomains in mammalian cells. We believe that ESI/MS-facilitated lipidomics has now opened a critical door that will greatly increase our understanding of human disease. -Han, X., and R. W. Gross. Lipidomics is a rapidly expanding research field fueled by recent advances in, and novel applications of, electrospray ionization mass spectrometry (ESI/MS). Lipidomics is focused on identifying alterations in lipid metabolism and lipid-mediated signaling processes that regulate cellular homeostasis during health and disease. Research in lipidomics incorporates multiple techniques to quantify the precise chemical constituents in a cell's lipidome, identify their cellular organization (subcellular membrane compartments and domains), delineate the biochemical mechanisms through which lipids interact with each other and with crucial membrane-associated proteins, determine lipid-lipid and lipid-protein conformational space and dynamics, and quantify alterations in lipid constituents after cellular perturbations. Through the detailed quantification of a cell's lipidome (e.g., lipid classes, subclasses, and individual molecular species), the kinetics of lipid metabolism, and the interactions of lipids with cellular proteins, lipidomics has already provided new insights into health and disease. The true power and promise of lipidomics, however, is only beginning to be realized.Decades of painstaking research in the 1970s and 1980s developed a straight and reversed-phase HPLC system that could "rapidly" (30 min) separate phospholipid classes, molecular species, and regioisomers into individual chemical constituents (1-6). These techniques, however, were labor intensive and required sensitive separation methods for which quantitation was difficult, because the → * transition during UV detection was not strictly proportional to mass content (7). Moreover, these procedures could not meet the needs for the application of lipidomi...

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“…In addition, fast atom bombardment (FAB) [8,9] as well as field desorption (FD) [10 -12] Although being the longest established method for ionic analyte analysis, FD has been overlooked in this scenario of mass spectral techniques for IL analysis. Field desorption (FD) is known since the 1970s as a soft desorption/ionization method in mass spectrometry [10, 28 -30].…”

mentioning

confidence: 99%

“…In addition, fast atom bombardment (FAB) [8,9] as well as field desorption (FD) [10 -12] are capable of delivering the required analytical information. Indeed, analyses of ILs with MALDI [13], ESI [14 -17], and FAB [18] have already been conducted, and those methods were found to serve nicely for the analysis of ILs.…”

mentioning

confidence: 99%

Liquid injection field desorption/ionization-mass spectrometry of ionic liquids

Gross

2007

J. Am. Soc. Mass Spectrom.

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Ten ionic liquids based on four types of organic cations, C ϩ (imidazolium, pyrrolidinium, pyridinium, and phosphonium), combined with various types of anions, AϪ, were analyzed by liquid injection field desorption/ionization-(LIFDI) mass spectrometry. For the purpose of LIFDI analysis the ionic liquids were dissolved in methanol, acetonitrile or tetrahydrofuran at concentrations of 0.01-0.1 l mL Ϫ1 . The measurements were performed on a double-focusing magnetic sector instrument. In all ionic liquid LIFDI spectra, the intact cation of the compound yielded the base peak accompanied by cluster ions of the general formula [C 2 A] ϩ and occasionally [C 3 A 2 ] ϩ . Tandem mass spectrometry and reconstructed ion chromatograms were employed to reveal the identity of the observed ions. Although limited to positive-ion mode, LIFDI also provided analytical information on the anions due to cluster ion formation. Depending on actual emitter condition and ionic liquid the limit of detection in survey scans was determined to 5-50 pg of ionic liquid. [1][2][3]. Consequently, there is a need for the development of methods for their analysis, be it for the sake of checking their purity, to verify their absence in products from processes where ILs are involved, or to monitor their distribution in the environment as a result of their long-term usage.Mass spectrometry (MS) offers various methods for the analysis of ionic analytes, with electrospray ionization (ESI) [4,5] and matrix-assisted laser desorption/ ionization (MALDI) [6,7] presently being the widest known techniques for that purpose. In addition, fast atom bombardment (FAB) [8,9] as well as field desorption (FD) [10 -12] Although being the longest established method for ionic analyte analysis, FD has been overlooked in this scenario of mass spectral techniques for IL analysis. Field desorption (FD) is known since the 1970s as a soft desorption/ionization method in mass spectrometry [10, 28 -30]. FD-MS has proven capabilities in analyzing nonionic low-to medium polarity compounds [12].FD employs strong electric fields in the order of 10 10 V m Ϫ1 (1 V Å Ϫ1 ) to effect the ionization of atoms and molecules [11,12,31]. The necessary field strength is normally achieved by setting whisker-bearing wiresso-called activated emitters [32][33][34]-to high electric potential of typically 10 -12 kV opposed to a counter electrode at ϳ2 mm distance. With the emitter at positive polarity, electron tunneling becomes possible from the analyte residing on the activated emitter's large surface into the emitter material. The analyte is thereby ionized very softly and the molecular ions generated are immediately extracted into the mass analyzer by action of the same electric field.Due to the advent of liquid injection field desorption/ionization (LIFDI) [35][36][37] the FD experiment can now be conducted easier, faster, and with more reproducibility than with the previous methodology of sample supply outside the ion source. If required, LIFDI offers fully inert sample transfer from se...

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Secondary ion mass spectrometry with cesium ion primary beam and liquid target matrix for analysis of bioorganic compounds

Cited by 290 publications

References 27 publications

Lipid Composition of Plasma Membranes and Endomembranes Prepared from Roots of Barley (Hordeum vulgare L.)

Lipid Composition of Plasma Membranes and Endomembranes Prepared from Roots of Barley (Hordeum vulgare L.)

Global analyses of cellular lipidomes directly from crude extracts of biological samples by ESI mass spectrometry: a bridge to lipidomics

Liquid injection field desorption/ionization-mass spectrometry of ionic liquids

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