Secondary dormancy in<i>Brassica napus</i>is correlated with enhanced<i>BnaDOG1</i>transcript levels

Née, Guillaume; Obeng-Hinneh, Evelyn; Sarvari, Pourya; Soppe, Wim J. J.

doi:10.1017/s0960258514000427

Cited by 15 publications

(16 citation statements)

References 32 publications

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“…Née et al . () also detected BnaDOG1 transcripts during seed development, but concluded that expression of BnaDOG1 was not sufficient for induction of dormancy. However, the highest BnaDOG1 transcript levels were found between about 37 and 44 DAP, and they decreased to very low levels from 51 to 56 DAP.…”

Section: Are Brassica Napus Seeds Nondormant At Maturity?mentioning

confidence: 99%

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A review of the relationship between primary and secondary dormancy, with reference to the volunteer crop weed oilseed rape (Brassica napus)

Soltani

Baskin

2018

Weed Research

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Fresh seeds of oilseed rape (Brassica napus) are reported to be nondormant and nonphotoblastic. However, a portion of the seeds can be induced into a light-requiring state (secondary dormancy) for germination and also exhibit dormancy cycling. Thus, if seeds become buried in the soil they can form a persistent seedbank and become a serious volunteer weed in succeeding crops. The capacity of nondormant seeds of B. napus to be induced into secondary dormancy is contrary to results of studies on fresh nondormant seeds of some other species. A reanalysis of published and unpublished data shows that fresh seeds of this species have some degree of primary dormancy and that there is a significant relationship between primary dormancy and the capacity to enter secondary dormancy. However, most germination tests on B. napus have not been done in enough detail to detect primary dormancy (or not) in fresh seeds of this species. The usefulness of information on the relationship between primary dormancy and the capacity of the seeds to enter secondary dormancy is discussed in relation to management of weedy volunteers of this species.

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Section: Are Brassica Napus Seeds Nondormant At Maturity?mentioning

confidence: 99%

“…Née et al . () reported that the gene BnaDOG1 ( B. napus DELAY OF GERMINATION1 ) plays a role in the induction of secondary dormancy in B. napus . Née et al .…”

Section: Are Brassica Napus Seeds Nondormant At Maturity?mentioning

confidence: 99%

A review of the relationship between primary and secondary dormancy, with reference to the volunteer crop weed oilseed rape (Brassica napus)

Soltani

Baskin

2018

Weed Research

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“…On the other hand, although dog1 loss-of-function mutants have a strong non-dormant phenotype (Bentsink et al 2006), it does not exclude that there may exist some factor(s) different to DOG1 required in triggering and maintaining of dormancy which still is present during AR0 imbibition. This possibility was recently conjectured in B. napus for the induction of primary dormancy (Née et al 2015). Interestingly, the AtDOG1-mediated seed dormancy of Arabidopsis is regulated, at least in part, at the level of the AtDOG1 transcripts (Bentsink et al 2006).…”

Section: Discussionmentioning

confidence: 90%

“…AR7). Previous work has shown that: (1) AtDOG1 ‐mRNA remains present in AR dry seeds although its transcript levels quickly drop during imbibition (Bentsink et al , Nakawayashi et al ); (2) AtDOG1, with an estimated molecular mass of 40 kDa, is localized in the nuclei of the embryo, accumulates during seed maturation and remains stable during AR (Nakawayashi et al ); (3) AtDOG1 protein levels in freshly harvested seeds correlate with the AR time required to relieve dormancy (Nakawayashi et al , Graeber et al ); (4) BnaDOG1 ‐mRNA was also detected during seed maturation of B. napus despite its very low level of primary dormancy suggesting that the DOG1 expression is not sufficient for dormancy induction (Née et al ); (5) LesaDOG1 has functions beyond seed dormancy (i.e. sensu stricto germination; Graeber et al ).…”

Section: Discussionmentioning

confidence: 99%

ABA‐stimulated SoDOG1 expression is after‐ripening inhibited during early imbibition of germinating Sisymbrium officinale seeds

Carrillo-Barral

Matilla

García-Ramas

et al. 2015

Physiologia Plantarum

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DELAY OF GERMINATION 1 (AtDOG1) was the first gene identified as dormancy-associated, but its physiological role in germination is far from being understood. Here, an orthologue of AtDOG1 in Sisymbrium officinale (SoDOG1; KM009050) is being reported. Phylogenetically, the SoDOG1 gene is included into the dicotyledonous group together with DOG1 from Arabidopsis thaliana (EF028470), Brassica rapa (AC189537), Lepidium papillosum (JX512183, JX512185) and Lepidium sativum (GQ411192). The SoDOG1 expression peaked at the onset of the silique maturation stage and there was presence of SoDOG1-mRNA in the freshly collected viable dry seed (i.e. AR0). The SoDOG1 transcripts were also found in other organs, such as open and closed flowers and to a lesser degree in roots and stems. We have previously reported in S. officinale seeds in which sensu stricto germination is positively affected by nitrate and both testa and micropylar endosperm ruptures are temporally separated. In dry viable seeds, the SoDOG1-mRNA level in three different after-ripening (AR) status was AR0 ≈ AR7 (optimal AR) < AR27 (optimal AR was almost lost). The presence of nitrate in the AR0 seed imbibition medium markedly decreased the SoDOG1 expression during sensu stricto germination. However, the nitrate stimulated the SoDOG1 expression during imbibition of AR7 compared to AR0. At the early AR0 seed imbibition (3-6 h), exogenous ABA provoked a very strong stimulation of the SoDOG1 expression. AR inhibits ABA-induced SoDOG1 expression during early germination and gibberellins (GA) can partially mimic this AR effect. A view on the integration of all found results in the sensu stricto germination of S. officinale was conducted.

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“…Murphey et al [19] proposed that DOG1 is a relevant gene in the induction of SD in response to temperatures. In some seeds, DOG1 induces SD because of the warm and prolonged cold stratification Plants 2020, 9, 480 5 of 20 in the course of seed imbibition [20,39]. Finch-Savage and Footitt indicated in their recent update on PD [34] that the thermo-inhibition of germination was DOG1 dependent and not reliant on an increased amount of ABA in lab conditions; while DOG1 is of decisive importance to dormancy cycling in field conditions in addition to its importance in determining the extent of PD.…”

Section: Dog1 In Seed Maturation and Dormancymentioning

confidence: 99%

Delay of Germination-1 (DOG1): A Key to Understanding Seed Dormancy

Carrillo-Barral

Rodríguez-Gacio

Matilla

2020

Plants

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DELAY OF GERMINATION-1 (DOG1), is a master regulator of primary dormancy (PD) that acts in concert with ABA to delay germination. The ABA and DOG1 signaling pathways converge since DOG1 requires protein phosphatase 2C (PP2C) to control PD. DOG1 enhances ABA signaling through its binding to PP2C ABA HYPERSENSITIVE GERMINATION (AHG1/AHG3). DOG1 suppresses the AHG1 action to enhance ABA sensitivity and impose PD. To carry out this suppression, the formation of DOG1-heme complex is essential. The binding of DOG1-AHG1 to DOG1-Heme is an independent processes but essential for DOG1 function. The quantity of active DOG1 in mature and viable seeds is correlated with the extent of PD. Thus, dog1 mutant seeds, which have scarce endogenous ABA and high gibberellin (GAs) content, exhibit a non-dormancy phenotype. Despite being studied extensively in recent years, little is known about the molecular mechanism underlying the transcriptional regulation of DOG1. However, it is well-known that the physiological function of DOG1 is tightly regulated by a complex array of transformations that include alternative splicing, alternative polyadenylation, histone modifications, and a cis-acting antisense non-coding transcript (asDOG1). The DOG1 becomes modified (i.e., inactivated) during seed after-ripening (AR), and its levels in viable seeds do not correlate with germination potential. Interestingly, it was recently found that the transcription factor (TF) bZIP67 binds to the DOG1 promoter. This is required to activate DOG1 expression leading to enhanced seed dormancy. On the other hand, seed development under low-temperature conditions triggers DOG1 expression by increasing the expression and abundance of bZIP67. Together, current data indicate that DOG1 function is not strictly limited to PD process, but that it is also required for other facets of seed maturation, in part by also interfering with the ethylene signaling components. Otherwise, since DOG1 also affects other processes such us flowering and drought tolerance, the approaches to understanding its mechanism of action and control are, at this time, still inconclusive.Plants 2020, 9, 480 2 of 20 (e.g., temperature, O 2 and light) [1][2][3][4]. PD dormancy is a notable agronomic feature. Thus, low levels may produce pre-harvest sprouting, while high levels inhibit the rapid and uniform germination rate. In both cases, there is a reduction of crop production [1,4,5]. PD is established and maintained in the viable dry seed, and throughout several molecular paths (e.g., after-ripening (AR) and exposure to gentle cooling) and can be broken gradually [6,7]. On the other hand, non-dormant seeds of various species have been reported to achieve secondary dormancy (SD) upon exposure to unfavorable conditions for germination [1]. SD occurs essentially after seed dispersal and may be induced by environmental interactions or other special conditions. DELAY OF GERMINATION1 (DOG1), a heme-binding protein, was identified in seeds of A. thaliana by using a biparental recombinant inbred popul...

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Secondary dormancy inBrassica napusis correlated with enhancedBnaDOG1transcript levels

Cited by 15 publications

References 32 publications

A review of the relationship between primary and secondary dormancy, with reference to the volunteer crop weed oilseed rape (Brassica napus)

A review of the relationship between primary and secondary dormancy, with reference to the volunteer crop weed oilseed rape (Brassica napus)

ABA‐stimulated SoDOG1 expression is after‐ripening inhibited during early imbibition of germinating Sisymbrium officinale seeds

Delay of Germination-1 (DOG1): A Key to Understanding Seed Dormancy

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