Secondary-blast injury in rodents produces cognitive sequelae and distinct motor recovery trajectories

Gamboa, Jasmine; Horváth, J.; Simon, Amanda A.; Islam, Md. Shiful; Gao, Sijia; Perk, Dror; Thoman, Amy; Calderon, Diany P

doi:10.1016/j.brainres.2020.147275

Cited by 2 publications

(10 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We provided analgesic (Flunixin 2.5 mg/Kg) and saline postoperatively. We monitored weight and temperature over the course of 7 days, as previously reported ( 18 ). Animals were placed alone in cages to facilitate recovery and EEG/EMG monitoring.…”

Section: Methodsmentioning

confidence: 99%

“…We previously described an SBI mouse model ( 18 ) in which we modeled injuries caused by flying debris generated by an explosion ( 20 ). Briefly, an air blast was generated using a 6-gallon portable electric air compressor attached to a nail gun (no nails were present).…”

Section: Methodsmentioning

confidence: 99%

“…The air blast triggered by the device propelled a piston (shrapnel) toward an ~2 × 2 mm area of the brain that included the frontal/prefrontal cortical-pallidal thalamocortical loops. The blast also resulted in degeneration in distant areas, including the midbrain and brainstem regions ( 18 ). Animals were induced with isoflurane (4% vol) and maintained with a (1.50% vol).…”

Section: Methodsmentioning

confidence: 99%

“…We imported data to Matlab (Mathworks). Data was filtered from noise artifacts and the delta (2-4 Hz), theta (5-8 Hz), alpha (8-13 Hz), beta (16)(17)(18)(19)(20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30)(31), and gamma (32-100 Hz) power were calculated using the Thomson multitaper method implemented in the Chronux toolbox in Matlab (21,22). To compute cortical spectrograms, we used the function mtspecgramc with the following parameters: frequency band = [0, 150 Hz], tapers = [3,5], movingwin = [5, 0.1] s. For the EMG, we applied the following parameters: frequency band [5,170 Hz] and tapers [3,5].…”

Section: Detection Of Sleep/wake Cycle Stagesmentioning

confidence: 99%

“…We previously generated a mouse model of secondary blast injury (SBI) in the brain ( 18 ) using local high-pressure waves that propel shrapnel, and studied cognitive, motor and mood deficits. SBIs are injuries caused by fragments and debris propelled by high-pressure waves during explosions ( 19 ).…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Dexmedetomidine-mediated sleep phase modulation ameliorates motor and cognitive performance in a chronic blast-injured mouse model

Bibineyshvili

Schiff²,

Calderon³

2022

Front. Neurol.

View full text Add to dashboard Cite

Multiple studies have shown that blast injury is followed by sleep disruption linked to functional sequelae. It is well established that improving sleep ameliorates such functional deficits. However, little is known about longitudinal brain activity changes after blast injury. In addition, the effects of directly modulating the sleep/wake cycle on learning task performance after blast injury remain unclear. We hypothesized that modulation of the sleep phase cycle in our injured mice would improve post-injury task performance. Here, we have demonstrated that excessive sleep electroencephalographic (EEG) patterns are accompanied by prominent motor and cognitive impairment during acute stage after secondary blast injury (SBI) in a mouse model. Over time we observed a transition to more moderate and prolonged sleep/wake cycle disturbances, including changes in theta and alpha power. However, persistent disruptions of the non-rapid eye movement (NREM) spindle amplitude and intra-spindle frequency were associated with lasting motor and cognitive deficits. We, therefore, modulated the sleep phase of injured mice using subcutaneous (SC) dexmedetomidine (Dex), a common, clinically used sedative. Dex acutely improved intra-spindle frequency, theta and alpha power, and motor task execution in chronically injured mice. Moreover, dexmedetomidine ameliorated cognitive deficits a week after injection. Our results suggest that SC Dex might potentially improve impaired motor and cognitive behavior during daily tasks in patients that are chronically impaired by blast-induced injuries.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Detection Of Sleep/wake Cycle Stagesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Dexmedetomidine-mediated sleep phase modulation ameliorates motor and cognitive performance in a chronic blast-injured mouse model

Bibineyshvili

Schiff²,

Calderon³

2022

Front. Neurol.

View full text Add to dashboard Cite

show abstract

Sleep, inflammation, and hemodynamics in rodent models of traumatic brain injury

Green,

Carey,

Mannino

et al. 2024

Front. Neurosci.

View full text Add to dashboard Cite

Traumatic brain injury (TBI) can induce dysregulation of sleep. Sleep disturbances include hypersomnia and hyposomnia, sleep fragmentation, difficulty falling asleep, and altered electroencephalograms. TBI results in inflammation and altered hemodynamics, such as changes in blood brain barrier permeability and cerebral blood flow. Both inflammation and altered hemodynamics, which are known sleep regulators, contribute to sleep impairments post-TBI. TBIs are heterogenous in cause and biomechanics, which leads to different molecular and symptomatic outcomes. Animal models of TBI have been developed to model the heterogeneity of TBIs observed in the clinic. This review discusses the intricate relationship between sleep, inflammation, and hemodynamics in pre-clinical rodent models of TBI.

show abstract

Secondary-blast injury in rodents produces cognitive sequelae and distinct motor recovery trajectories

Cited by 2 publications

References 43 publications

Dexmedetomidine-mediated sleep phase modulation ameliorates motor and cognitive performance in a chronic blast-injured mouse model

Dexmedetomidine-mediated sleep phase modulation ameliorates motor and cognitive performance in a chronic blast-injured mouse model

Sleep, inflammation, and hemodynamics in rodent models of traumatic brain injury

Contact Info

Product

Resources

About