Second Report of Hands-on Seminar on Basic Research for Clinicians at the 54th Annual Meeting of the Japanese Rhinologic Society

Abstract

We previously reported on a hands-on seminar on basic research organized at the rd Annual Meeting of the Japanese Rhinologic Society Osaka, Japan in order to not only improve individual skills and knowledge but also enhance interaction and collaboration between researchers. A questionnaire survey was also conducted following this seminar. Moreover, we investigated the shift in the number of basic oral and poster presentations at the annual meetings of the Japanese Rhinologic Society from to. In this previous report, % of participants agreed that the seminar was useful and most hoped to continue such seminars in the future. The percentage of basic oral and poster presentations at the meeting decreased to % compared with % at the meeting. Thus, to further develop this seminar on basic research, we again organized at the th Annual Meeting of the Japanese Rhinologic Society Hiroshima, Japan a second hands-on seminar on basic research for clinicians, focusing on how to prepare good frozen sections, obtain good DNA and RNA, and perform reverse transcription polymerase chain reaction RT-PCR. Here, we present an outline of this second seminar and discuss detailed procedures for performing Kawamoto's film method, which is a new technique with adhesive film for the preparation of multipurpose fresh-frozen sections from hard tissues, and for collecting DNA and RNA from nasal tissue. Additionally, we present the results of a questionnaire survey and the percentage of basic oral and poster presentations in this seminar as last year, as well as discuss perspective of this trial.