Second-harmonic imaging of the absolute polar molecular orientation at interfaces

Flörsheimer, M.; Bootsmann, M.-T.; Fuchs, Harald

doi:10.1103/physrevb.65.125406

Cited by 10 publications

(5 citation statements)

References 41 publications

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“…In general, unfortunately, when laser beams are scanned angu- larly by a galvano mirror system through an objective lens, they essentially travel different path lengths within highly dispersive lens media for different angular positions. 9 This effect is more pronounced especially for very thick lenses such as high-NA objectives made of a stack of many lenses with sophisticate designs. This resembles the situation of changing PSU thickness, and consequently results in a reduced field of view within which the interferometric effort pays.…”

Section: Resultsmentioning

confidence: 99%

“…However, a strong nonresonant background might sometimes be very useful in terms of enhancing the resonant signal through a homodyne interferometric amplification and consequently improving the image quality. [8][9][10] In this paper, we set up a nonlinear optical interferometer to see how CARS imaging can be improved by interferometric implementations. The experimental scheme is different from conventional schemes that have been tried elsewhere for imaging purpose.…”

Section: Introductionmentioning

confidence: 99%

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Nonlinear optical interference of two successive coherent anti-Stokes Raman scattering signals for biological imaging applications

Lee

Yoo

2007

J. Biomed. Opt.

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The nonlinear optical interference of two successively generated coherent anti-Stokes Raman scattering (CARS) signals from two different samples placed in series is demonstrated for the imaging performance, in which a collinear phase matching geometry is used. The relative phase of two CARS signals is controlled by a phase-shifting unit made of dispersive glass materials of which the thickness can be precisely varied. The clear interference fringes are observed as the thickness of the phase-shifting unit changes. The interference effect is then utilized to achieve a better quality CARS image of a biological tissue taken from a mouse skin. Placing the tissue in the second sample position and performing raster scans of the laser beams on it, we can acquire a CARS image of higher contrast compared to the normal image obtained without interferometric implementation.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Nonlinear optical interference of two successive coherent anti-Stokes Raman scattering signals for biological imaging applications

Lee

Yoo

2007

J. Biomed. Opt.

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“…Surface-SHG (S-SHG) uses TIR of a pulsed IR beam so that the SHG efficiency of the surface can be evaluated selectively. S-SHG is a well-established optical technique for studying non-linear materials with high surface/interface specificity [95][96][97][98]. Again, the use of a gold film on the interface can be used to provide a signal enhancement [99].…”

Section: Surface Second-harmonic Generationmentioning

confidence: 99%

Non-linear evanescent-field imaging

Oheim¹,

Schapper²

2005

J. Phys. D: Appl. Phys.

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Total internal reflection fluorescence (TIRF), a general term that embraces any spectroscopic or microscopic technique based on the evanescent field created by TIR of light, is further establishing itself as an important tool for studying near-surface phenomena. Impingement of a femtosecond-pulsed infrared beam on a reflecting interface creates the conditions for ‘macroscopic’ evanescent-field two-photon fluorescence excitation. The two-photon fluorescence excitation volume is confined by both the non-linearity of the multi-photon process and the spatial inhomogeneity of the evanescent field. The absence of scattered excitation resulting in a low background and the possibility of simultaneous multi-colour fluorescence excitation should make non-linear evanescent-field excitation particularly attractive for quantitative single-molecule observation and ultra-sensitive screening assays. In this topical review, we survey the requirements, present the current results and explore the potential of this novel non-linear microscopy.

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“…Over the past twenty years, many spectroscopic techniques have been utilized to investigate the average molecular orientation at the interface, such as second-harmonic generation, [8][9][10][11] fluorescence, 12 attenuated total internal reflection, 12,13 linear dichroism, 12,14,15 and photoacoustic spectroscopy. 11 Recently, cavity ring-down spectroscopy (CRDS) has also been used to carry out molecular orientation studies.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular Orientation Study of Methylene Blue at an Air/Fused-Silica Interface Using Evanescent-Wave Cavity Ring-Down Spectroscopy

Zare

2005

J. Phys. Chem. B

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Using evanescent-wave cavity ring-down spectroscopy (EW-CRDS), we monitored the change in the absorbance of a thin film of methylene blue (MB) at an air/fused-silica interface while varying the polarization of the incident light (600 nm). We derived the average orientation angle of the planar MB molecules with respect to the surface normal and observed that the average orientation angle decreases as the surface concentration increases. At low surface concentrations, the MB molecules lie almost flat on the surface, whereas at higher surface concentrations the molecules become vertically oriented.

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Second-harmonic imaging of the absolute polar molecular orientation at interfaces

Cited by 10 publications

References 41 publications

Nonlinear optical interference of two successive coherent anti-Stokes Raman scattering signals for biological imaging applications

Nonlinear optical interference of two successive coherent anti-Stokes Raman scattering signals for biological imaging applications

Non-linear evanescent-field imaging

Molecular Orientation Study of Methylene Blue at an Air/Fused-Silica Interface Using Evanescent-Wave Cavity Ring-Down Spectroscopy

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