Second Harmonic Generation (SHG) microscopy of articular cartilage to image osteoarthritis

Houle, Marie-Andrée; Brown, Cameron; Popov, Konstantin; Nicklaus, Mischa; Ruediger, Andréas; Chen, Minsi; Price, Andrew; Ramunno, Lora; Gill, Harinderjit; Légaré, François

doi:10.1364/fio.2012.ftu3a.38

Cited by 1 publication

(1 citation statement)

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“…Kiyomatsu et al, presented a quantitative approach to characterize induced OA in a mice model [14]. Additionally, a few other reports use NLOM to study degenerative cartilage, but the analysis is rudimentary [15][16][17]. Due to different load-bearing tendency in a synovial joint and the slow progression of OA, the pathophysiology in humans may differ from animal models and remains to be explored [18,19].…”

Section: Introductionmentioning

confidence: 99%

Nonlinear optical microscopy of early stage (ICRS Grade-I) osteoarthritic human cartilage

Kumar

Grønhaug

Davies

et al. 2015

Biomed. Opt. Express

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Abstract:In a synovial joint, the articular cartilage is directly affected during the progression of Osteoarthritis (OA). The characterization of early stage modification in extra-cellular matrix of cartilage is essential for detection as well as understanding the progression of disease. The objective of this study is to demonstrate the potential and capability of nonlinear optical microscopy for the morphological investigation of early stage osteoarthritic cartilage. ICRS Grade-I cartilage sections were obtained from the femoral condyle of the human knee. The surface of articular cartilage was imaged by second harmonic generation and two-photon excited fluorescence microscopy. Novel morphological features like microsplits and wrinkles were observed, which would otherwise not be visible in other clinical imaging modalities (e.g., CT, MRI, ultrasound and arthroscope. The presence of superficial layer with distinct collagen fibrils parallel to the articular surface in 4 specimens out of 14 specimens, indicates that different phases of OA within ICRS Grade-I can be detected by SHG microscopy. All together, the observed novel morphologies in early stage osteoarthritic cartilage indicates that SHG microscopy might be a significant tool for the assessment of cartilage disorder.

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