Second-generation ethanol from non-detoxified sugarcane hydrolysate by a rotting wood isolated yeast strain

Bazoti, Suzana F.; Golunski, Simone Maria; Siqueira, Diego Pereira; Scapini, Thamarys; Barrilli, Évelyn Taize; Mayer, Diego A.; Barros, Katharina O; Rosa, Carlos A.; Stambuk, Boris U.; Alves, Sérgio L.; Valério, Alexsandra; Oliveira, Débora de; Treichel, Helen

doi:10.1016/j.biortech.2017.08.007

Cited by 50 publications

(27 citation statements)

References 24 publications

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“…In addition, C. pseudointermedia and Wickerhamomyces sp. were able to ferment sugarcane bagasse hydrolysates without detoxification, obtaining ethanol concentrations of 11 g/L at a 72 h fermentation, with a 0.15 g/L·h productivity (Bazoti et al, 2017). This is a lower value than that reported for corn stover (0.22 g/L·h), and similar to that obtained in mango peel-seed residues in this study.…”

Section: Mango (Seed-peel) Wheat Strawsupporting

confidence: 83%

“…The study of yeasts that are capable of using pentoses, mainly xylose, becomes relevant (Bellasio et al, 2015). Yeast members of the Candida genus are widely reported as a xylose fermenter (Schirmer-Michel et al, 2008;Martins et al, 2018;Pérez-Cadena et al, 2018;Shariq and Sohail, 2018), as well as yeasts of the Pichia, Kluyveromyces (Mussatto et al, 2012) and Wickerhamomyces (Bazoti et al, 2017) genera.…”

Section: Molecular Identification Of Selected Yeastsmentioning

confidence: 99%

“…Martins et al (2018) conducted a search for yeasts capable of fermenting synthetic xylose into ethanol, finding the C. tropicalis S4 strain which metabolizes 56 g/L of synthetic xylose to produce 6 g/L of ethanol. In addition, yeasts of the Candida and Wickerhamomyces genera are able to ferment sugars obtained from hydrolysis of pretreated sugarcane bagasse by steam explosion into ethanol; however, the strains show a preference for glucose, by consuming it almost entirely, but not for other sugars present in the hydrolysate (Bazoti et al, 2017). Similarly, Pichia kudriavzevii 2-KLP1 yeast is able to metabolize sugars obtained from the acid hydrolysate of agroindustrial residues such as wheat bran, sugarcane bagasse, rice husk, and sawdust (Elahi and Rehman, 2018).…”

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confidence: 99%

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Valorisation of agroindustrial residues acid hydrolyzates as carbon sources for ethanol production by native yeast strains with different fermentative capabilities//Valorización de hidrolizados ácidos de residuos agroindustriales como fuente de carbono para la producción de etanol por levaduras nativas con capacidades fermentativas diferentes

et al. 2020

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A profitable, second-generation (2G) bioethanol production process requires the use of the maximum amountof sugars present in the lignocellulosic biomass; among them are those obtained from hemicellulose hydrolysis. An alternative is the search and kinetic characterization of yeasts capable of fermenting xylose to ethanol. In this study, 161 yeasts were isolated from agroindustrial residues, and selected according to best growth in glucose and xylose. Five strains belonging to the genera Candida (C. intermedia and C. parapsilosis), and Wickerhamomyces (W. anomalus) were molecularly identified. The kinetic parameters indicate that C. intermedia CBE002 had the best biomass yield in glucose and xylose (0.21 and 0.35 g/g of substrate), maximum specific growth rate (0.15 and 0.12 h-1) and metabolized both sugars simultaneously, desirable characteristics and rarely found together in other yeasts. Bioethanol production was made possible by C. intermedia (CBE002) from acid hydrolysates of corn stover and mango residues, with yields of 0.31 and 0.26 g/g of substrate, respectively. From the results obtained, this yeast is an attractive candidate to be used in bioethanol 2G production, and to take advantage of the large amount of agroindustrial residues available.RESUMENUn proceso de producción de bioetanol de segunda generación (2G) rentable, requiere el uso del máximo número de azúcares presentes en la biomasa lignocelulósica, como son los obtenidos por hidrólisis de hemicelulosa; para obtenerlo, una alternativa es encontrar levaduras capaces de fermentar eficientemente xilosa a etanol. En el presente trabajo se realizó el aislamiento de 161 levaduras a partir de residuos agroindustriales, se evaluó su capacidad de crecimiento en glucosa y xilosa. Se seleccionaron e identificaron molecularmente cinco de estas cepas pertenecientes a los géneros Candida (C. intermedia, C. parapsilosis) y Wickerhamomyces (W. anomalus). Los parámetros cinéticos demostraron que C. intermedia CBE002 obtuvo el mejor rendimiento de biomasa en glucosa y xilosa (0.21 y 0.35 g/g), la máxima velocidad específica de crecimiento (0.15 y 0.12 h-1) y fue capaz de metabolizar ambos azúcares simultáneamente, característica deseable y poco encontrada en otras levaduras. Fue posible la producción de bioetanol por C. intermedia CBE002 a partir de hidrolizados ácidos de rastrojo de maíz y residuos de mango, con rendimientos de 0.31 y 0.26 g/g de sustrato, respectivamente. Por lo anterior, esta levadura es atractiva para ser empleada en la producción de bioetanol 2G y aprovechar la gran cantidad de residuos agroindustriales disponibles.

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Section: Mango (Seed-peel) Wheat Strawsupporting

confidence: 83%

Section: Molecular Identification Of Selected Yeastsmentioning

confidence: 99%

mentioning

confidence: 99%

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Valorisation of agroindustrial residues acid hydrolyzates as carbon sources for ethanol production by native yeast strains with different fermentative capabilities//Valorización de hidrolizados ácidos de residuos agroindustriales como fuente de carbono para la producción de etanol por levaduras nativas con capacidades fermentativas diferentes

et al. 2020

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“…Liquid chromatography-Mass spectrometer (SHIMADZU, Japan) was used in determining sugars and acetic acid in a refractive index detector (DIR-10A) with a specialized column (AMINEX ® BIORAD HPX87H) with 0.005 mol L À1 sulfuric acid as mobile phase and other conditions being: isocratic mode, temperature of 45 C, 20 mL injected into the detector at a flow of 0.6 mL min À1 . Each compound was then determined using calibration curves benchmarked against LC-MS standards (Sigma-Aldrich) [45]. Same procedure was used to determine the concentrations of furfural and hydroxymethylfurfural (HMF) with a slight modification by attaching a diode array detector (DAD) to the LC-MS while a C18 column was used with 1:8 acetonitrile/water to which 1% acetic acid has been added as the mobile phase.…”

Section: Analyses Of Structural Componentsmentioning

confidence: 99%

Liquefaction of pineapple peel: Pretreatment and process optimization

Dahunsi

2019

Energy

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a b s t r a c tThis study explored the optimization of pretreatment of pineapple peel for biogas generation. Pretreatments were carried out sulfuric acid and alkaline hydrogen peroxide prior to anaerobic digestion while the response surface methodology (RSM) was used for optimization of the pretreatment procedures. The physical, chemical, proximate and structural compositions of the peels were determined prior to and at the end of the pretreatment procedures. The dynamics of microorganisms in the reactors were also evaluated by rapid molecular methods while the Fourier Transform Infra-red (FTIR) spectroscopy was employed in the identification of the chemical changes as a result of pretreatments. The use of H 2 O 2 pretreatment caused enormous lignin solubilization in the pineapple peel. In comparison, biogas production was 67% more in the alkaline pretreated pineapple peel than the biomass treated with acid and also 51% over the untreated samples. The total biogas volume produced from the acidic pretreated, alkaline pretreated, not sifted untreated and sifted untreated samples are 194.2 ± 3.0, 587.5 ± 5.2, 287.8 ± 2.1 and 245.4 ± 3.1 respectively. Thus, the alkaline pretreated experiment used lower retention time to achieve maximum gas production in this study. The use of alkaline H 2 O 2 on lignocelluloses has remained unpopular prior to this study. However, its usage in this study yielded better result than all the conventional treatments in terms of lignin solubilization and improvement in methane yield. Economically, the use of H 2 O 2 for pretreatment is adjudged feasible because the 1504 kWh t À1 TS thermal energy gain obtained from the biogas produced by the alkaline treated peel exceeded the 921 kWh t À1 TS used in the pretreatment. This gives a net thermal energy of 583 kWh t À1 TS. Whereas, the investment into acidic pretreatment of pineapple peel may not be economically justified because the total thermal energy gain of À200 kWh t À1 TS was far lower than the 1236 kWh t À1 TS thermal energy that was consumed during the pretreatment giving a net thermal energy of À1436 kWh t À1 TS. Therefore, the use of mild alkaline pretreatment is advocated in biogas generation from pineapple peel and also for biofertilizer production mostly in localities of mass production.

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“…In addition, it is capable of inhibiting yeast xylose metabolism (decreasing xylose reductase activity), and even at relatively low concentrations (5.4 g/L) it strongly inhibits xylitol production by Candida tropicalis (Rafiqul, Sakinah, & Zularisam, ). Thus, identification of microorganisms with higher tolerance to inhibitors, especially acetic acid, is essential for the development of an efficient xylitol production process (Bazoti et al, ; Xu & Hanna, ).…”

Section: Introductionmentioning

confidence: 99%

Xylitol production on sugarcane biomass hydrolysate by newly identified Candida tropicalis JA2 strain

Morais

Pacheco

Trichez

et al. 2019

Yeast

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Xylitol is a building block for a variety of chemical commodities, besides being widely used as a sugar substitute in the food and pharmaceutical industries. The aim of this work was to develop a microbial process for xylitol production using sugarcane bagasse hydrolysate as substrate. In this context, 218 non-Saccharomyces yeast strains were screened by growth on steam-exploded sugarcane bagasse hydrolysate containing a high concentration of acetic acid (8.0 g/L). Seven new Candida tropicalis strains were selected and identified, and their ability to produce xylitol on hydrolysate at low pH (4.6) under aerobic conditions was evaluated.The most efficient strain, designated C. tropicalis JA2, was capable of producing xylitol with a yield of 0.47 g/g of consumed xylose. To improve xylitol production by C. tropicalis JA2, a series of experimental procedures were employed to optimize pH and temperature conditions, as well as nutrient source, and initial xylose and inoculum concentrations. C. tropicalis JA2 was able to produce 109.5 g/L of xylitol with a yield of 0.86 g/g of consumed xylose, and with a productivity of 2.81 g·L·h, on sugarcane bagasse hydrolysate containing 8.0 g/L acetic acid and177 g/L xylose, supplemented with 2.0 g/L yeast nitrogen base and 4.0 g/L urea. Thus, it was possible to identify a new C. tropicalis strain and to optimize the xylitol production process using sugarcane bagasse hydrolysate as a substrate. The xylitol yield on biomass hydrolysate containing a high concentration of acetic acidobtained in here is among the best reported in the literature.

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Second-generation ethanol from non-detoxified sugarcane hydrolysate by a rotting wood isolated yeast strain

Cited by 50 publications

References 24 publications

Liquefaction of pineapple peel: Pretreatment and process optimization

Xylitol production on sugarcane biomass hydrolysate by newly identified Candida tropicalis JA2 strain

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