Second and third trimester amniotic fluid mesenchymal stem cells can repopulate a de-cellularized lung scaffold and express lung markers

Vadasz, Stephanie; Jensen, Todd; Moncada, Camilo; Girard, Eric; Zhang, Fan; Blanchette, Alex; Finck, Christine

doi:10.1016/j.jpedsurg.2014.04.006

Cited by 26 publications

(21 citation statements)

References 77 publications

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“…We demonstrated that hAFSCs are positive for CD44 and CD90 whereas negative for CD34 and CD45, which is in accordance with the recommendations of the International Society for Cytotherapy, both on the transcript and protein level for MSCs cells identification. Results obtained in the present study are comparable with the results of other research groups [Kim et al, ; DeKoninck et al, ; Sousa et al, ; Vadasz et al, ]. Additionally, we showed the presence of pluripotency markers (hNanog, OCT3/4, and SOX2) in hAFSCs, however, only on the low level.…”

Section: Discussionsupporting

confidence: 93%

High Quality Independent From a Donor: Human Amniotic Fluid Derived Stem Cells—A Practical Analysis Based on 165 Clinical Cases

Bajek

Olkowska

Walentowicz-Sadlecka

et al. 2016

J of Cellular Biochemistry

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The aim of the study was to extend the potential use of human stem cells isolated from amniotic fluid in medical applications by confirming their high homogeneity and quality. Amniotic fluid samples were collected during amniocentesis from 165 women during pregnancy. The proliferation rate, clonogenicity, karyotype, aging process, pluripotent cell markers, expression of surface markers, and the potential to differentiate into adipose, bone and cartilage cells of hAFSCs were analyzed. Obtained results revealed that mesenchymal stem cells could be derived successfully from amniotic fluid, which exhibit properties comparable with MSCs of other origins. It is the first study, in which such a large group of patients was involved. Comprehensive statistical and biological analysis were conducted some of which clearly being innovative in relation to human amniotic fluid-derived stem cells. J. Cell. Biochem. 118: 116-126, 2017. © 2016 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 93%

High Quality Independent From a Donor: Human Amniotic Fluid Derived Stem Cells—A Practical Analysis Based on 165 Clinical Cases

Bajek

Olkowska

Walentowicz-Sadlecka

et al. 2016

J of Cellular Biochemistry

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“…Characterization of marker expression of stem cells was realized using the Fluorescence-activated cell sorting (FACS) method [23,24]). hAMSCs were positive for many surface markers including CD44, CD90 and also positive for transcription factors such Oct-4, Rex1, whereas they were negative for CD34, CD45 and CD117 in agreement with previous reports [25][26][27]. This confirmed that Dual enzyme (collagenase II and DNAase I) also ensured high activity of hAMSCs.…”

Section: Isolation and Primary Culture Of Hamscssupporting

confidence: 90%

Enhanced efficiency in isolation and expansion of hAMSCs via dual enzyme digestion and micro-carrier

Gohi

Liu

et al. 2020

Cell Biosci

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A two-stage method of obtaining viable human amniotic stem cells (hAMSCs) in large-scale is described. First, human amniotic stem cells are isolated via dual enzyme (collagenase II and DNAase I) digestion. Next, relying on a culture of the cells from porous chitosan-based microspheres in vitro, high purity hAMSCs are obtained in large-scale. Dual enzymatic (collagenase II and DNase I) digestion provides a primary cell culture and first subculture with a lower contamination rate, higher purity and a larger number of isolated cells. The obtained hAMSCs were seeded onto chitosan microspheres (CM), gelatin-chitosan microspheres (GCM) and collagen-chitosan microspheres (CCM) to produce large numbers of hAMSCs for clinical trials. Growth activity measurement and differentiation essays of hAMSCs were realized. Within 2 weeks of culturing, GCMs achieved over 1.28 ± 0.06 × 10 7 hAMSCs whereas CCMs and CMs achieved 7.86 ± 0.11 × 10 6 and 1.98 ± 0.86 × 10 6 respectively within this time. In conclusion, hAMSCs showed excellent attachment and viability on GCM-chitosan microspheres, matching the hAMSCs' normal culture medium. Therefore, dual enzyme (collagenase II and DNAase I) digestion may be a more useful isolation process and culture of hAMSCs on porous GCM in vitro as an ideal environment for the large-scale expansion of highly functional hAMSCs for eventual use in stem cell-based therapy.

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“…This cell source has an extraordinary expansion potential from as little as 2-5 mL of fluid. Furthermore, it has been verified in vivo that these cells do not produce teratomas in comparison to pluripotent stem cells [47,54]. Studies have described differences in these AF-MSCs based on gestational age; however, a recent study demonstrated that cells isolated from amniotic fluid at full term still contain a large population of MSCs [55].…”

Section: Discussionmentioning

confidence: 99%

“…Approximately 10 mL of amniotic fluid was collected by the resident veterinarian and sent to our laboratory for processing approximately 4 weeks prior to birth. The amniotic fluid was centrifuged for 5 min at 400 g and processed [46,47]. The cell pellet was suspended in 10 mL of amniotic fluid medium.…”

Section: Isolation Of Af-mscs From Amniotic Fluid Obtained Via Amniocmentioning

confidence: 99%

Polyurethane scaffolds seeded with autologous cells can regenerate long esophageal gaps: An esophageal atresia treatment model

Jensen

Wanczyk

Sharma

et al. 2019

Journal of Pediatric Surgery

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Background: Pediatric patients suffering from long gap esophageal defects or injuries are in desperate need of innovative treatment options. Our study demonstrates that two different cell sources can adhere to and proliferate on a retrievable synthetic scaffold. In feasibility testing of translational applicability, these cell seeded scaffolds were implanted into piglets and demonstrated esophageal regeneration. Methods: Either porcine esophageal epithelial cells or porcine amniotic fluid was obtained and cultured in 3 dimensions on a polyurethane scaffold (Biostage). The amniotic fluid was obtained prior to birth of the piglet and was a source of mesenchymal stem cells (AF-MSC). Scaffolds that had been seeded were implanted into their respective Yucatan mini-swine. The cell seeded scaffolds in the bioreactor were evaluated for cell viability, proliferation, genotypic expression, and metabolism. Feasibility studies with implantation evaluated tissue regeneration and functional recovery of the esophagus. Results: Both cell types seeded onto scaffolds in the bioreactor demonstrated viability, adherence and metabolism over time. The seeded scaffolds demonstrated increased expression of VEGF after 6 days in culture. Once implanted, endoscopy 3 weeks after surgery revealed an extruded scaffold with newly regenerated tissue. Both cell seeded scaffolds demonstrated epithelial and muscle regeneration and the piglets were able to eat and grow over time. Conclusions: Autologous esophageal epithelial cells or maternal AF-MSC can be cultured on a 3D scaffold in a bioreactor. These cells maintain viability, proliferation, and adherence over time. Implantation into piglets demonstrated esophageal regeneration with extrusion of the scaffold. This sets the stage for translational application in a neonatal model of esophageal atresia.

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Second and third trimester amniotic fluid mesenchymal stem cells can repopulate a de-cellularized lung scaffold and express lung markers

Cited by 26 publications

References 77 publications

High Quality Independent From a Donor: Human Amniotic Fluid Derived Stem Cells—A Practical Analysis Based on 165 Clinical Cases

High Quality Independent From a Donor: Human Amniotic Fluid Derived Stem Cells—A Practical Analysis Based on 165 Clinical Cases

Enhanced efficiency in isolation and expansion of hAMSCs via dual enzyme digestion and micro-carrier

Polyurethane scaffolds seeded with autologous cells can regenerate long esophageal gaps: An esophageal atresia treatment model

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