Second- and third-generation biodiesel production with immobilised recombinant Rhizopus oryzae lipase: Influence of the support, substrate acidity and bioprocess scale-up

López-Fernández, Josu; Benaiges, M. Dolors; Valero, Francisco

doi:10.1016/j.biortech.2021.125233

Cited by 23 publications

(7 citation statements)

References 47 publications

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“…Consistent with our findings in a continuous bioreactor, high operational stability has been reported for lipases used in batch mode, even in the presence of olive pomace oil with high acidity [ 38 ]. López-Fernández et al [ 50 ] observed that long-chain free fatty acids had a positive effect on the stability of a recombinant Rhizopus oryzae lipase immobilized on a resin, when used in batch transesterification of crude acidic olive pomace oil (19% acidity) or other crude oils with ethanol or methanol, for biofuel production. They concluded that the operational stability of the biocatalyst depended more on the oil type utilized than on its acidity.…”

Section: Resultsmentioning

confidence: 99%

Continuous Production of Dietetic Structured Lipids Using Crude Acidic Olive Pomace Oils

et al. 2023

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Crude olive pomace oil (OPO) is a by-product of olive oil extraction. In this study, low-calorie structured triacylglycerols (TAGs) were produced by acidolysis of crude OPO with medium-chain fatty acids (caprylic, C8:0; capric, C10:0) or interesterification with their ethyl ester forms (C8EE, C10EE). These new TAGs present long-chain fatty acids (L) at position sn-2 and medium-chain fatty acids (M) at positions sn-1,3 (MLM). Crude OPO exhibited a high acidity (12.05–28.75% free fatty acids), and high contents of chlorophylls and oxidation products. Reactions were carried out continuously in a packed-bed bioreactor for 70 h, using sn-1,3 regioselective commercial immobilized lipases (Thermomyces lanuginosus lipase, Lipozyme TL IM; and Rhizomucor miehei lipase, Lipozyme RM IM), in solvent-free media at 40 °C. Lipozyme RM IM presented a higher affinity for C10:0 and C10EE. Lipozyme TL IM preferred C10:0 over C8:0 but C8EE over C10EE. Both biocatalysts showed a high activity and operational stability and were not affected by OPO acidity. The New TAG yields ranged 30–60 and the specific productivity ranged 0.96–1.87 g NewTAG/h.g biocatalyst. Lipozyme RM IM cost is more than seven-fold the Lipozyme TL IM cost. Therefore, using Lipozyme TL IM and crude acidic OPO in a continuous bioreactor will contribute to process sustainability for structured lipid production by lowering the cost of the biocatalyst and avoiding oil refining.

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Section: Resultsmentioning

confidence: 99%

Continuous Production of Dietetic Structured Lipids Using Crude Acidic Olive Pomace Oils

et al. 2023

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“… Network visualization map for the co-occurrence of works that bring information about the scale-up process using lipases applied to bioprocesses [ 41 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 ]. Publications in the period from 2018 to 2022 were considered.…”

Section: Figurementioning

confidence: 99%

Trends in the Use of Lipases: A Systematic Review and Bibliometric Analysis

Santos

Vieira

Almeida

et al. 2023

Foods

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Scientific mapping using bibliometric data network analysis was applied to analyze research works related to lipases and their industrial applications, evaluating the current state of research, challenges, and opportunities in the use of these biocatalysts, based on the evaluation of a large number of publications on the topic, allowing a comprehensive systematic data analysis, which had not yet been conducted in relation to studies specifically covering lipases and their industrial applications. Thus, studies involving lipase enzymes published from 2018 to 2022 were accessed from the Web of Science database. The extracted records result in the analysis of terms of bibliographic compatibility among the articles, co-occurrence of keywords, and co-citation of journals using the VOSviewer algorithm in the construction of bibliometric maps. This systematic review analysis of 357 documents, including original and review articles, revealed studies inspired by lipase enzymes in the research period, showing that the development of research, together with different areas of knowledge, presents good results related to the applications of lipases, due to information synchronization. Furthermore, this review showed the main challenges in lipase applications regarding increased production and operational stability; establishing well-defined evaluation criteria, such as cultivation conditions, activity, biocatalyst stability, type of support and reactor; thermodynamic studies; reuse cycles; and it can assist in defining goals for the development of successful large-scale applications, showing several points for improvement of future studies on lipase enzymes.

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“…The enzymatic production of biodiesel has several advantages in terms of process sustainability over its chemical production. However, only exceptionally stable biocatalysts allow cost-effective production [45,46]. This led us to assess the potential of the truncated prosequence of ROL for increasing the operational stability of the resulting biocatalyst under enzymatic biodiesel production conditions and its influence on the initial transesterification rate.…”

Section: Biodiesel Productionmentioning

confidence: 99%

Constitutive Expression in Komagataella phaffii of Mature Rhizopus oryzae Lipase Jointly with Its Truncated Prosequence Improves Production and the Biocatalyst Operational Stability

2021

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Rhizopus oryzae lipase (ROL) containing 28 C-terminal amino acids of the prosequence fused to the N-terminal mature sequence in ROL (proROL) was successfully expressed in the methylotrophic yeast Komagataella phaffii (Pichia pastoris) under the constitutive glyceraldehyde-3-phosphate dehydrogenase promoter (PGAP). Although the sequence encoding the mature lipase (rROL) was also transformed, no clones were obtained after three transformation cycles, which highlights the importance of the truncated prosequence to obtain viable transformed clones. Batch cultures of the K. phaffii strain constitutively expressing proROL scarcely influenced growth rate and exhibited a final activity and volumetric productivity more than six times higher than those obtained with proROL from K. phaffii under the methanol-inducible alcohol oxidase 1 promoter (PAOX1). The previous differences were less marked in fed-batch cultures. N-terminal analysis confirmed the presence of the 28 amino acids in proROL. In addition, immobilized proROL exhibited increased tolerance of organic solvents and an operational stability 0.25 and 3 times higher than that of immobilized rROL in biodiesel and ethyl butyrate production, respectively. Therefore, the truncated prosequence enables constitutive proROL production, boosts bioprocess performance and provides a more stable biocatalyst in two reactions in which lipases are mostly used at industrial level, esterification (ethyl butyrate) and transesterification (biodiesel).

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Second- and third-generation biodiesel production with immobilised recombinant Rhizopus oryzae lipase: Influence of the support, substrate acidity and bioprocess scale-up

Cited by 23 publications

References 47 publications

Continuous Production of Dietetic Structured Lipids Using Crude Acidic Olive Pomace Oils

Continuous Production of Dietetic Structured Lipids Using Crude Acidic Olive Pomace Oils

Trends in the Use of Lipases: A Systematic Review and Bibliometric Analysis

Constitutive Expression in Komagataella phaffii of Mature Rhizopus oryzae Lipase Jointly with Its Truncated Prosequence Improves Production and the Biocatalyst Operational Stability

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