(−)-Secoisolariciresinol attenuates high-fat diet-induced obesity in C57BL/6 mice

Abstract

Flaxseed lignan, secoisolariciresinol has been reported to possess health benefits. We previously synthesized each stereoisomer of secoisolariciresinol and found that (-)-secoisolariciresinol reduces lipid accumulation and induces adiponectin production in 3T3-L1 adipocytes. Here we show the effects of (-)-secoisolariciresinol on high-fat diet-induced obesity in C57BL/6 male mice. Oral administration of (-)-secoisolariciresinol for 28 consecutive days significantly suppressed the gain of body weight. Increased serum adiponectin level and decreased gene expression of fatty acid synthase and sterol regulatory element-binding protein-1c in liver, which are related to fatty acid synthesis, were observed in the mice orally administered with (-)-secoisolariciresinol. In addition, subcutaneous injection of (-)-secoisolariciresinol also significantly suppressed the gain of body weight. Serum leptin levels were significantly increased by treating with (-)-secoisolariciresinol or (-)-enterolactone. Subcutaneous injection of (-)-secoisolariciresinol, (-)-enterolactone, or (-)-enterodiol promoted gene expression of acyl-CoA oxidase, carnitine palmitoyl transferase-1, and peroxisome proliferator-activated receptor α, which are related to β-oxidation. Overall results suggest that (-)-secoisolariciresinol exerts a suppressive effect on the gain of body weight of mice fed a high-fat diet by inducing gene expression of adiponectin, resulting in the altered expression of various genes related to the synthesis and β-oxidation of fatty acids.

Flaxseed Consumption Inhibits Chemically Induced Lung Tumorigenesis and Modulates Expression of Phase II Enzymes and Inflammatory Cytokines in A/J Mice

“…Flaxseeds contain ~ 30% dietary fibers, consumption of which has been shown to reduce fat digestibility and body weight gain in rats [38]. In addition, flaxseed lignan secoisolariciresinol diglucoside exerts a suppressive effect on body weight gain in mice by altering lipid metabolism [39]. Our result is in line with the effect of flaxseed on body weight gain reported earlier in rodents.…”

“…This suggest that even though initially the flaxseed group mice were reluctant to eat the fortified diet, they became accustomed to it and consumed it as well as the control diet during the majority of the study. The flaxseed-fed mice had lower average body weight at the end of the study which could be attributed to, (i) reduced food consumption in the first two months of the study, (ii) reduced fat digestibility [38], and/or (iii) altered adipogenesis-gene regulation [39]. The decrease in average weight of lungs in the flaxseed fed mice as compared to control group mice suggest tumor burden may be a contributing factor.…”

Effects of an equol‐producing bacterium isolated from human faeces on isoflavone and lignan metabolism in mice

“…Additionally, high‐fat diet‐induced visceral and liver fat accumulation was significantly reduced by administration of SDG to mice . SECO might reduce lipid accumulation by changing the expression of various genes related to the synthesis and β ‐oxidation of fatty acids . In our study, a negative correlation was observed between the visceral fat per final body weight and the urinary amounts of SECO of the two groups ( r = −0.54).…”

Distinct signatures of host–microbial meta-metabolome and gut microbiome in two C57BL/6 strains under high-fat diet

“…Obese C57N mice showed also lower levels of enterolactone in cecum. Subcutaneous injection of enterolactone and enteridiol tend to decrease body weight in mice (Tominaga et al, 2012). The patterns of urobilinoids are likely due to different communities that were observed in C57J and C57N mice by our 16S-rRNA gene analyses.…”